Acetylation of CspC Controls the Las Quorum-Sensing System through Translational Regulation of
            <i>rsaL</i>
            in Pseudomonas aeruginosa

Li, Shouyi; Gong, Xuetao; Yin, Liwen; Pan, Xiaolei; Jin, Yongxin; Bai, Fang; Cheng, Zhihui; Ha, Un-Hwan; Wu, Weihui

doi:10.1128/mbio.00547-22

Cited by 6 publications

(4 citation statements)

References 76 publications

(98 reference statements)

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“…Typhimurium can sense succinate to promote antimicrobial resistance and T3SS secretion ( 40 ). The accumulation of itaconate leads to decreased lipopolysaccharide production and decreased acetylation levels of CspC in P. aeruginosa ( 42 , 43 ). During infection, E. piscicida manipulates host arginine metabolism to interfere with inflammation occurrence ( 44 ).…”

Section: Discussionmentioning

confidence: 99%

Lysine acetylation regulates the AT-rich DNA possession ability of H-NS

Liu,

Zhou,

et al. 2023

Nucleic Acids Research

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H-NS, the histone-like nucleoid-structuring protein in bacteria, regulates the stability of the bacterial genome by inhibiting the transcription of horizontally transferred genes, such as the type III and type VI secretion systems (T3/T6SS). While eukaryotic histone posttranslational modifications (PTMs) have been extensively studied, little is known about prokaryotic H-NS PTMs. Here, we report that the acetylation of H-NS attenuates its ability to silence horizontally transferred genes in response to amino acid nutrition and immune metabolites. Moreover, LC−MS/MS profiling showed that the acetyllysine sites of H-NS and K120 are indispensable for its DNA-binding ability. Acetylation of K120 leads to a low binding affinity for DNA and enhances T3/T6SS expression. Furthermore, acetylation of K120 impairs the AT-rich DNA recognition ability of H-NS. In addition, lysine acetylation in H-NS modulates in vivo bacterial virulence. These findings reveal the mechanism underlying H-NS PTMs and propose a novel mechanism by which bacteria counteract the xenogeneic silencing of H-NS.

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Section: Discussionmentioning

confidence: 99%

Lysine acetylation regulates the AT-rich DNA possession ability of H-NS

Liu,

Zhou,

et al. 2023

Nucleic Acids Research

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“…Besides its role in QS, RsaL is also a global regulator controlling the expression of numerous genes involved in bacterial functions such as virulence, including the phz1 cluster, phzM, and cdpR, and in antibiotic resistance, including the narK1K2GHJI operon 8, 14,[17][18][19] . Consequently, the rsaL mutation in the P. aeruginosa strain PAO1 enhances the production of virulence factors, such as elastase, hemolysins, hydrogen cyanide, and phenazine metabolites, increases ciprofloxacin and carbenicillin resistance and decreases the ability of the bacteria to cause chronic lung infections in mice 15,[19][20][21][22] . Therefore, understanding the RsaL-dependent regulation of gene expression may uncover new ways to control the harmful effects of P. aeruginosa in clinics and agriculture.…”

Section: Introductionmentioning

confidence: 99%

“…Consequently, the rsaL mutation in the P . aeruginosa strain PAO1 enhances the production of virulence factors, such as elastase, hemolysins, hydrogen cyanide, and phenazine metabolites, increases ciprofloxacin and carbenicillin resistance and decreases the ability of the bacteria to cause chronic lung infections in mice 15 , 19 , 20 , 21 , 22 . Therefore, understanding the RsaL‐dependent regulation of gene expression may uncover new ways to control the harmful effects of P .…”

Section: Introductionmentioning

confidence: 99%

RsaL is a self‐regulatory switch that controls alternative biosynthesis of two AHL‐type quorum sensing signals in Pseudomonas aeruginosa PA1201

He,

Jin,

Cui

et al. 2024

mLife

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Pseudomonas aeruginosa is a ubiquitous and metabolically versatile microorganism naturally found in soil and water. It is also an opportunistic pathogen in plants, insects, animals, and humans. In response to increasing cell density, P. aeruginosa uses two acyl‐homoserine lactone (AHL) quorum‐sensing (QS) signals (i.e., N‐3‐oxo‐dodecanoyl homoserine lactone [3‐oxo‐C12‐HSL] and N‐butanoyl‐homoserine lactone [C4‐HSL]), which regulate the expression of hundreds of genes. However, how the biosynthesis of these two QS signals is coordinated remains unknown. We studied the regulation of these two QS signals in the rhizosphere strain PA1201. PA1201 sequentially produced 3‐oxo‐C12‐HSL and C4‐HSL at the early and late growth stages, respectively. The highest 3‐oxo‐C12‐HSL‐dependent elastase activity was observed at the early stage, while the highest C4‐HSL‐dependent rhamnolipid production was observed at the late stage. The atypical regulator RsaL played a pivotal role in coordinating 3‐oxo‐C12‐HSL and C4‐HSL biosynthesis and QS‐associated virulence. RsaL repressed lasI transcription by binding the –10 and –35 boxes of the lasI promoter. In contrast, RsaL activated rhlI transcription by binding the region encoding the 5′‐untranslated region of the rhlI mRNA. Further, RsaL repressed its own expression by binding a nucleotide motif located in the –35 box of the rsaL promoter. Thus, RsaL acts as a molecular switch that coordinates the sequential biosynthesis of AHL QS signals and differential virulence in PA1201. Finally, C4‐HSL activation by RsaL was independent of the Las and Pseudomonas quinolone signal (PQS) QS signaling systems. Therefore, we propose a new model of the QS regulatory network in PA1201, in which RsaL represents a superior player acting at the top of the hierarchy.

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“… 49 In addition, CspC can upregulate Las-QS by binding to the 5′ UTR (untranslated regions) of rsaL mRNA. 50 …”

Section: Introductionmentioning

confidence: 99%

The transcriptional regulators of virulence for Pseudomonas aeruginosa: Therapeutic opportunity and preventive potential of its clinical infections

et al. 2023

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Acetylation of CspC Controls the Las Quorum-Sensing System through Translational Regulation of rsaL in Pseudomonas aeruginosa

Cited by 6 publications

References 76 publications

Lysine acetylation regulates the AT-rich DNA possession ability of H-NS

Lysine acetylation regulates the AT-rich DNA possession ability of H-NS

RsaL is a self‐regulatory switch that controls alternative biosynthesis of two AHL‐type quorum sensing signals in Pseudomonas aeruginosa PA1201

The transcriptional regulators of virulence for Pseudomonas aeruginosa: Therapeutic opportunity and preventive potential of its clinical infections

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