Structure and Dimensions of Core–Shell Nanoparticles Comparable to the Confocal Volume Studied by Means of Fluorescence Correlation Spectroscopy

Gapiński, Jacek; Jarzębski, Maciej; Buitenhuis, Johan; Deptuła, Tobiasz; Mazuryk, Jarosław; Patkowski, A.

doi:10.1021/acs.langmuir.5b04605

Cited by 11 publications

(6 citation statements)

References 16 publications

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“…We applied the core–shell type of nanoparticles to avoid the impact of nanoparticle size on FCS measurements. 32 , 33 Full information on tracers used in the experiments is presented in Supporting Information 1 and 2 ( SI 1, SI 2 ). Cells filled with tracers at final concentrations of 1–100 nM in the cytoplasm were further examined under the confocal microscope.…”

Section: Length-scale Dependent Viscosity Of Cytoplasmmentioning

confidence: 99%

“…To confirm this prediction, tracers of defined hydrodynamic radii, ranging from 0.65 to 81 nm, were introduced to cytoplasmic area of cells via microinjection (dextrans and nanoparticles), passive inflow (Calcein-AM), or biosynthesis upon transfection (proteins) (Figure , panel II). We applied the core–shell type of nanoparticles to avoid the impact of nanoparticle size on FCS measurements. , Full information on tracers used in the experiments is presented in Supporting Information 1 and 2 (SI 1, SI 2). Cells filled with tracers at final concentrations of 1–100 nM in the cytoplasm were further examined under the confocal microscope.…”

Section: Length-scale Dependent Viscosity Of Cytoplasmmentioning

confidence: 99%

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Nanoscale Viscosity of Cytoplasm Is Conserved in Human Cell Lines

Kwapiszewska

Szczepański

Kalwarczyk

et al. 2020

J. Phys. Chem. Lett.

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Metabolic reactions in living cells are limited by diffusion of reagents in the cytoplasm. Any attempt to quantify the kinetics of biochemical reactions in the cytosol should be preceded by careful measurements of the physical properties of the cellular interior. The cytoplasm is a complex, crowded fluid characterized by effective viscosity dependent on its structure at a nanoscopic length scale. In this work, we present and validate the model describing the cytoplasmic nanoviscosity, based on measurements in seven human cell lines, for nanoprobes ranging in diameters from 1 to 150 nm. Irrespective of cell line origin (epithelial–mesenchymal, cancerous–noncancerous, male–female, young–adult), we obtained a similar dependence of the viscosity on the size of the nanoprobes, with characteristic length-scales of 20 ± 11 nm (hydrodynamic radii of major crowders in the cytoplasm) and 4.6 ± 0.7 nm (radii of intercrowder gaps). Moreover, we revealed that the cytoplasm behaves as a liquid for length scales smaller than 100 nm and as a physical gel for larger length scales.

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Section: Length-scale Dependent Viscosity Of Cytoplasmmentioning

confidence: 99%

Section: Length-scale Dependent Viscosity Of Cytoplasmmentioning

confidence: 99%

Nanoscale Viscosity of Cytoplasm Is Conserved in Human Cell Lines

Kwapiszewska

Szczepański

Kalwarczyk

et al. 2020

J. Phys. Chem. Lett.

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“…From the Stokes -Einstein relationship, = 6 we obtain the hydrodynamic diameter (DH) of the particles. Recently, for colloids and nanoparticles that have diameters that are comparable in size to the confocal volume a correction is needed [28,29]:…”

Section: Fluorescence Correlation Spectroscopymentioning

confidence: 99%

Click-based thiol-ene photografting of COOH groups to SiO2 nanoparticles: Strategies comparison

Penelas

Soler-Illia

Levi

et al. 2019

Colloids and Surfaces A: Physicochemical and Engineering Aspect

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We present the study of the anchoring of carboxylic groups on SiO2 nanoparticles from different approximations based on the photochemical radical thiol-ene addition (PRTEA) reaction: a photografting approach between mercaptosuccinic acid (MSA) and vinyl-modified SiO2 nanoparticles and the post-grafting on the surface of silica colloids of the silane precursor 2-((2-(trimethoxysilyl)ethyl)thio)succinic acid (TMSMSA), obtained from the PRTEA. These synthetic strategies were compared with a widely common derivatization methodology based on the nucleophilic attack of surface-anchored amino groups with succinic anhydride. The successful functionalization of the colloidal silica was confirmed by infrared spectroscopy (FTIR), zeta potential at different pH and contact angle measurements. We found that although these three approaches were valid for -COOH immobilization, they had a noticeable impact on the dispersability and agglomeration of the colloidal suspension at the end of the synthesis. Scanning electron microscopy, dynamic light scattering (DLS) and fluorescence correlation spectroscopy (FCS) measurements indicated that the PRTEA photografting between MSA and vinyl-modified SiO2 resulted in highly dispersed colloidal particles. On the other hand, the presence of surface -COOH groups was highly beneficial for redispersion of the colloidal material after lyophilization or freeze-drying procedures.

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“…Their unique physical, optical, and electronic features may provide high activity and unusual properties through nanotechnology means [1]. It seems clear that in recent years nanoparticles are popular not only with respect to their potential bioapplications (i.e., drug delivery, biosensors, and bioscaffolds) but also due to the intense development of nanomaterials themselves resulting in numerous model systems as the probes to investigate and/or detect different physical phenomena [2][3][4]. None of the less polymeric nanoparticles have been widely used for biological applications including drug delivery since they must be responsive to external stimuli such as temperature and pH [5].…”

Section: Introductionmentioning

confidence: 99%

pNiPAM-Nanoparticle-Based Antiapoptotic Approach for Pro-Regenerative Capacity of Skeletal Myogenic Cells

et al. 2021

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The biocompatibility of pNiPAM (Poly N-isopropylacrylamide) copolymers has been examined and they did not exert any cytotoxic effects. Their properties and vulnerable temperature characteristics make them candidates for use in medical applications. We synthesized a well-characterized nanoparticles-based cargo system that would effectively deliver a biological agent to human skeletal myogenic cells (SkMCs); among other aspects, a downregulating apoptotic pathway potentially responsible for poor regeneration of myocardium. We confirmed the size of the pNiPAM based spheres at around 100 nm and the nanomeric shape of nanoparticles (NP) obtained. We confirmed that 33 °C is the adequate temperature for phase transition. We performed the dynamics of cargo release. A small amount of examined protein was detected at 10 min after reaching LCTS (lower critical solution temperature). The presented results of the test with BSA (bovine serum albumin) and doxorubicin loaded into nanoparticles showed a similar release profile for both substances. SkMCs incubated with NP loaded with antiapoptotic agent, BCB (Bax channel blocker), significantly diminished cell apoptosis (p < 0.01). Moreover, the lowest apoptotic level was detected in SkMCs treated with camptothecin and simultaneously incubated with pNiPAMs loaded with BCB. Application of nanoparticles loaded with BCB or subjected to BCB alone did not, however, diminish the amount of apparently necrotic cells.

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Structure and Dimensions of Core–Shell Nanoparticles Comparable to the Confocal Volume Studied by Means of Fluorescence Correlation Spectroscopy

Cited by 11 publications

References 16 publications

Nanoscale Viscosity of Cytoplasm Is Conserved in Human Cell Lines

Nanoscale Viscosity of Cytoplasm Is Conserved in Human Cell Lines

Click-based thiol-ene photografting of COOH groups to SiO2 nanoparticles: Strategies comparison

pNiPAM-Nanoparticle-Based Antiapoptotic Approach for Pro-Regenerative Capacity of Skeletal Myogenic Cells

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