pNiPAM-Nanoparticle-Based Antiapoptotic Approach for Pro-Regenerative Capacity of Skeletal Myogenic Cells

Abstract

The biocompatibility of pNiPAM (Poly N-isopropylacrylamide) copolymers has been examined and they did not exert any cytotoxic effects. Their properties and vulnerable temperature characteristics make them candidates for use in medical applications. We synthesized a well-characterized nanoparticles-based cargo system that would effectively deliver a biological agent to human skeletal myogenic cells (SkMCs); among other aspects, a downregulating apoptotic pathway potentially responsible for poor regeneration of myocardium. We confirmed the size of the pNiPAM based spheres at around 100 nm and the nanomeric shape of nanoparticles (NP) obtained. We confirmed that 33 °C is the adequate temperature for phase transition. We performed the dynamics of cargo release. A small amount of examined protein was detected at 10 min after reaching LCTS (lower critical solution temperature). The presented results of the test with BSA (bovine serum albumin) and doxorubicin loaded into nanoparticles showed a similar release profile for both substances. SkMCs incubated with NP loaded with antiapoptotic agent, BCB (Bax channel blocker), significantly diminished cell apoptosis (p < 0.01). Moreover, the lowest apoptotic level was detected in SkMCs treated with camptothecin and simultaneously incubated with pNiPAMs loaded with BCB. Application of nanoparticles loaded with BCB or subjected to BCB alone did not, however, diminish the amount of apparently necrotic cells.

Thermosensitive Behavior Defines the Features of Poly(N-isopropylacrylamide)/Magnetite Nanoparticles for Cancer Management

“…The lower critical solution temperature (LCST) of 32-34 • C for this "smart polymer" has made it useful for various applications such as valves and sensors, cell culture media, membranes, and drug delivery systems [35][36][37][38]. Numerous PNIPAM nanocarrier systems have been developed as candidates for use in biomedical applications [39][40][41][42][43]. The combined assembly of stimuli-responsive polymers with inorganic magnetic nanoparticles could exhibit properties for controlled delivery of doxorubicin into biological targets.…”