Structure and bioactivities of a polysaccharide isolated from Ganoderma lucidum in submerged fermentation

Abstract

In this study, a Ganoderma lucidum polysaccharide GLP-1-1 was isolated from a culture broth with Mw of 22014 Da. Monosaccharide contained glucose, mannose, and galactose with mole percentages of 92.33%, 7.55%, and 0.22%, respectively. Moreover, FTIR and methylation analysis were conducted to characterize the structural properties of GLP-1-1. The results of antioxidant activity analysis showed that GLP-1-1 had a great DPPH and ABTS radical scavenging activity. Meanwhile, GLP-1-1 also exhibited anti-tumor activity to A431 and MDA-MB-231 cells, and inhibitory rates were dose-dependent. During culturing with GLP-1-1, the G1/G0 cell percentage of A431 cells was increased from 48.64% to 84.52%, and the G1/G0 cell percentage of MDA-MB-231 cells was increased from 57.14% to 73.48%. Therefore, the anti-tumor activity of GLP-1-1 may be caused by inducing the G1/G0 arrest of tumor cells.

Utilization of Response Surface Methodology in Optimization of Polysaccharides Extraction from Vietnamese Red Ganoderma lucidum by Ultrasound-Assisted Enzymatic Method and Examination of Bioactivities of the Extract

“…In addition, glycosidic bonds might be also represented in the relatively weak absorption peaks at 1620 cm −1 [ 35 ]. Moreover, the stretching vibration of 879 cm −1 referred to the absorption of β -D-glucose with the pyranose form [ 35 , 36 ]. The other bands observed at 1086, 1044, and 879 cm −1 were characteristic absorptions of to β -(1,3), (1,6)-D-glucan [ 37 ].…”

A review of the therapeutic and biological effects of edible and wild mushrooms

“… Antifungal, Antioxidant, and Antiviral [ 182 , 183 ] 14 Craterellus cornucopioides black chanterelle, black trumpet, trumpet of the dead. North America, Europe, Japan, and Korea Antioxidant, Anti-hyperglycemic, Anti-inflammatory, Antimicrobial, and Immunomodulatory [ 184 , 185 ] 15 Craterellus tubaeformis yellow foot, winter mushroom, or funnel chanterelle Northern America, Europe, and Asia Anti-inflammatory and Antioxidant [ 186 , 187 ] 16 Flammulina velutipes Enokitake velvet shank, golden needle mushroom, or winter mushroom China, Japan, Korea, and Taiwan Antioxidant, Anticancer, Antihypertensive, Antimicrobial, immunomodulatory, and hepatoprotective [ 188 , 189 ] 17 Ganoderma lucidum Lingzhi (Ling chih)/Reishi Malaysia, China, Japan and South Korea Antioxidant, antitumor, Anti – androgenic and cardioprotective [ 190 ] 18 Grifola frondosa Hen of the Wood/Maitake Japan, China Europe and North America Antioxidant, Anti-diabetic, Anti-tumor, Immunomodulatory, antimicrobial, and hepatoprotective [ 116 , 191 ] 19 Gyromitra esculenta Brain mushroom, turban fungus, elephant ears, beefsteak mushroom Britain, Ireland, Europe, and North America Antioxidant, Anticholinesterase, and Antimicrobial [ 192 ,...…”

Amelioration effect of water extract from Ganoderma resinaceum FQ23 solid-state fermentation fungal substance with high-yield ergothioneine on anxiety-like insomnia mice

“…Numerous studies have shown that EGT, Ganoderma polysaccharides and phenolic substances have strong antioxidant activities. 21,43–47 Ganoderma triterpenoids have strong antitumor 48 and neuroprotective functions, 49,50 and also have the functions of protecting the liver, intestines, 51 and kidneys, 52 and regulating their related metabolism. 53 Ganoderma proteins have antioxidant, melanin inhibition and antibacterial functions, 54 and can protect DNA from hydroxyl radical damage.…”

“…Numerous studies have shown that EGT, Ganoderma polysaccharides and phenolic substances have strong antioxidant activities. 21,[43][44][45][46][47] Ganoderma triterpenoids have strong antitu-…”