Structure and 5′-termini of the large and 19 S RNA transcripts encoded by the cauliflower mosaic virus genome

Dudley, R. K.; Odell, Joan T.; Howell, Stephen H.

doi:10.1016/0042-6822(82)90503-7

Cited by 37 publications

(19 citation statements)

References 16 publications

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“…a1 has the same 5' terminus as the a-strand and is of the same polarity. Its 3' terminus falls at nucleotide 7437, just two nucleotides away from one of the major initiation sites for viral RNA transcription (Covey et al, 1981;Dudley et al, 1982;Guilley et al, 1982). We also show that about two-thirds of the molecules of a1 possess a ribonucleotide at their 5' terminus.…”

Section: Introductionmentioning

confidence: 87%

Observations concerning the discontinuous DNAs of cauliflower mosaic virus.

1983

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The double-stranded circular DNA encapsidated within cauliflower mosaic virus (CaMV) particles contains three single-stranded discontinuities, two in one strand and one in the other, so that, upon denaturation, three linear singlestranded DNAs are produced. Here we show that a fourth much smaller single-stranded DNA, termed a1, is also present in denatured CaMV DNA preparations. The 5' extremity of a, is identical to that of the a-strand, the strand of DNA possessing only one interruption, while its 3' extremity lies just two nucleotides downstream from a major transcription initiation site. We also show that the interrupted strand at each discontinuity sometimes has a single ribonucleotide in place of a deoxyribonucleotide at its 5' extremity. Oligoribonucleotide chains of eight and 10 residues in length have also been detected at the 5' end of one of the discontinuities. These structures are thought to be the vestiges of primers which have not been completely excised prior to encapsidation of the DNA. The possibility that synthesis of the astrand occurs by reverse transcription of viral RNA using initiator tRNAmet as primer is discussed.

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Section: Introductionmentioning

confidence: 87%

Observations concerning the discontinuous DNAs of cauliflower mosaic virus.

1983

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“…This is the mRNA for the gene product from open region VI (7,25,33). Detailed mapping (8,13,16) has positioned the 5'-and 3'-termini of this mRNA in the small and large non-coding regions respectively. Although the 35S RNA has been found in polysome preparations (25) the purified RNA does not translate in the reticulocyte cell-free system (16).…”

Section: Introductionmentioning

confidence: 99%

“…Its 3'-end maps coterminal with the 3'-end of the 19S mRNA and its 5'-end maps about 200 bases upstream of its 3'-end (8,13,16). In view of the features that CaMV has in common with retroviruses it has been suggested that the CaMV 35S RNA is the template for minus-strand DNA synthesis by reverse transcription (19,20,27).…”

Section: Introductionmentioning

confidence: 99%

A model for the expression of CaMV nucleic acid

Hull

1984

Plant Mol Biol

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“…In the absence of the FT0.35 fraction, the transcription products are much shorter (not shown). It can be seen that the fractions corresponding to the majority of the RNA polymerase activity synthesise very little RNA (fractions [12][13][14]. This may indicate that these fractions contain inhibitory activities which have been described in other systems [14].…”

Section: Transcription After Heparin-sepharose Fractionationmentioning

confidence: 82%

In vitro transcription from cauliflower mosaic virus promoters by a cell-free extract from tobacco cells

Cooke

Penon

1990

Plant Mol Biol

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We have studied transcription from the cauliflower mosaic virus 19S and 35S promoters in a cell-free system derived from tobacco cells in suspension culture. While a whole-cell extract is incapable of detectable transcription from these promoters, successive purification by column chromatography allows the preparation of two fractions which contain all factors necessary for transcription from the 19S promoter. In contrast, transcription from the 35S promoter leads to the accumulation of short RNAs. This accumulation can only be partially alleviated by modifying the conditions of transcription.

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Structure and 5′-termini of the large and 19 S RNA transcripts encoded by the cauliflower mosaic virus genome

Cited by 37 publications

References 16 publications

Observations concerning the discontinuous DNAs of cauliflower mosaic virus.

Observations concerning the discontinuous DNAs of cauliflower mosaic virus.

A model for the expression of CaMV nucleic acid

In vitro transcription from cauliflower mosaic virus promoters by a cell-free extract from tobacco cells

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