Structure-Activity Relationship Study of Synthetic Variants Derived from the Highly Potent Human Antimicrobial Peptide hLF(1-11)

Antimicrobial Activity of Lactoferrin-Related Peptides and Applications in Human and Veterinary Medicine

“…23 primers and probes and min. 15 primers and probes) • The FIC indexes of the checkerboard assays were all <1 showing that hLF (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11) and the antifungal drugs have a synergistic-additive relationship.…”

“…• Primer and Taqman probe design specific to a species or group of species that exhibit high specificity • Testing of primers and Taqman probes specificity in a singleplex format • Multiplexing the three different panels and assess specificity, LoD and efficiency of the reactions • EUCAST broth microdilution for the 121 isolates of Saccharomycotina yeasts commonly involved in candidemia • In vitro testing and determining hLF (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11) MICs against the isolates that exhibit high MICs to fluconazole and anidulafungin • In vitro combination testing of hLF (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11) and fluconazole or anidulafungin • Determining if the hLF (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11) and antifungal show a synergistic effect…”

Harnessing snake venom phospholipases A2 to novel approaches for overcoming antibiotic resistance

“…In fact, while the electrostatic interactions promoted by basic residues are related to selective recognition and binding to bacterial membranes, hydrophobic residues promote subsequent steps that are crucial for antibacterial activity (Páramo et al, 1998;Won & Ianoul, 2009). Cysteine has been found relevant for the activity of some AMP (Brouwer et al, 2018), and this is also observed for Lys49 svPLA 2 s Cterminal peptides as those depicted in Figure 3. These invariably possess a Cys residue at position 11, and assays with p-BthTX-I and its analogue where Cys was replaced by Ser showed a decrease in antimicrobial activity, probably due to the fact that the substituted peptide was no longer able to form a dimer resembling (p-BthTX-I) 2 (Santos-Filho et al, 2015).…”

Comparison of the Antibacterial Effect of Silver Nanoparticles and a Multifunctional Antimicrobial Peptide on Titanium Surface

“…Furthermore, applying a two-step functionalization (first depositing the AgNPs, followed by the deposition of the peptide platform) did not affect the density of the AgNPs retained on the surface, as the area covered by the NPs on Ti-Ag/PLATF is 30.8 ± 1.4%. This would indicate that this process is appropriate in order to immobilize both agents on the Ti surface without promoting a strong NPs agglomeration effect, which may occur due to electrostatic interactions with the positively charged peptide platform [35]. In Figure S1 (Supplementary information), it is shown that a combination of the peptide platform with the AgNPs in solution promotes the NP agglomeration, evidenced by the loss of the peak in the UV-vis spectrum, which is attributed to the possible high attraction of silver and the anchoring unit of the peptide platform (catechol groups).…”