Structurally diversified diterpenoids from Euphorbia dracunculoides

Wang, Li; Ma, Yue; Sun, Qianli; Li, Xiaonian; Yan, Yijun; Yang, Jing; Yang, Fu‐Mei; Liu, Fengyi; Zang, Zhen; Wu, Xianghua; Huang, Sheng‐Xiong; Zhao, Yong

doi:10.1016/j.tet.2015.06.078

Cited by 21 publications

(18 citation statements)

References 31 publications

(56 reference statements)

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“…On the other hand, the increased polarity of the extraction solvent allowed to observe the increase in the number of signals for carbinolic and olefinic protons present in polyhydroxylated compounds in the region between 3.0 and 6.0 ppm and the decrease of the signals in the spectral region between 0.5 and 2.5 ppm of the methyl groups of nonpolar compounds (Figure 1). [15,18] These inferences were supported by the GC/MS data, in which there is the suggestion of terpenes in dichloromethane (Dcm) and ethanol (Eth) fractions ( Figure 2). The GC/MS analysis and comparison with NIST and Wiley databases allowed the identification of some compounds present in the fractions; among them, euphol, the major compound already described in the Hex, and other triterpenes like lanosterol, cycloartenol, tirucallol, taraxasterol and lupeol, besides the sitosterol, a steroid already identified in this specie.…”

Section: Chemical Analysismentioning

confidence: 60%

Enriched Terpenes Fractions of the Latex of Euphorbia umbellata Promote Apoptosis in Leukemic Cells

Cruz

Kanunfre

Andrade

et al. 2020

Chemistry & Biodiversity

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The current study was carried out by a bioguided fractionation of a hexane extract of the latex of Euphorbia umbellata against leukemic cells. Samples were analyzed by NMR, GC/MS, triterpenes quantification, and MTT reduction assay. Morphological, cell cycle, mitochondrial membrane potential and caspases 3/7 analyses were performed for the dichloromethane and ethanol fractions, and selectivity index for the dichloromethane fraction. NMR analysis presented characteristic signals of terpenes and steroids, data were confirmed by the quantification of triterpenes and GC/MS analysis. MTT reduction assay demonstrated that HL‐60 was the most sensitive cell lineage against dichloromethane and ethanol fractions. Compounds of these matrices caused morphological changes compatible with apoptosis induction, altered cell cycle, increment of depolarized population cells and activation of caspases 3/7. Selectivity indices were higher than 22.44. Bioguided‐fractionation study showed that samples of the latex of E. umbellata raised the activity of the phytocomplex against leukemic cells, and the cytotoxicity can be associated with an apoptosis pathway.

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Section: Chemical Analysismentioning

confidence: 60%

Enriched Terpenes Fractions of the Latex of Euphorbia umbellata Promote Apoptosis in Leukemic Cells

Cruz

Kanunfre

Andrade

et al. 2020

Chemistry & Biodiversity

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“…The plants of Euphorbia contain more than 2000 species spread all over the world, and about 80 species distribute in China [ 1 , 2 ]. The dried root of Euphorbia kansui has long been used for the treatment of asthma, edema and ascites in traditional Chinese medicine.…”

Section: Introductionmentioning

confidence: 99%

Chemical Constituents from Euphorbia kansui

et al. 2017

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In this research, a new triterpenoid, tirucalla-8,24-diene-3β,11β-diol-7-one (1), and eupha-8,24-diene-3β,11β-diol-7-one (2), which was isolated from Euphorbia kansui for the first time, together with twelve other known compounds (3–14), were isolated from the ethyl acetate extract of Euphorbia kansui. Their structures were elucidated based on High resolution electrospray ionization mass spectrometry (HR-ESI-MS), Infrared Spectroscopy (IR), 1D and 2D Nuclear Magnetic Resonance (NMR) data. Both constituents 1 and 2 exhibited moderate cytotoxicity against colon cancer HCT-116, gastric cancer MKN-45 and breast cancer MCF-7.

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“…Euphorbia dracunculoides has been used by local practitioners for its diuretic and purgative properties. Structurally diversified 19 diterpenoids have been isolated from aerial parts of E. dracunculoides [ 16 ]. Because of similar morphology of the dried aerial parts of E. drancunculoides to Ruta graveolens , it is sold or used clinically as replacement of R. graveolens for analgesic and inflammatory disorders; gout and arthritis [ 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

Studies on phytochemical, antioxidant, anti-inflammatory and analgesic activities of Euphorbia dracunculoides

Majid

Khan

Shah

et al. 2015

BMC Complement Altern Med

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BackgroundPlants provide an alternative source to manage various human disorders due to diverse metabolites. Euphorbia dracunculoides of family Euphorbiaceae is used by local practitioners in rheumatism, epilepsy, edema, snake bite, warts and also possesses diuretic and purgative effects. The present study evaluated the antioxidant, anti-inflammatory and analgesic activities of various extracts of E. dracunculoides. Further, phytochemical constituents of the leading extracts were also investigated.MethodsDry powder of E. dracunculoides was extracted with n-hexane (EDH), acetone (EDA), ethanol (EDE), ethanol + water (1:1) (EDEW) and methanol (EDM) and screened for phytochemical classes, total phenolic (TPC) and flavonoid content (TFC). Antioxidant effects of the extracts were manifested by in vitro multidimensional assays. The anti-inflammatory and analgesic activities of the extracts were evaluated through carrageenan induced paw edema and hot plate test in rat. In addition, GC-MS analysis of EDH and HPLC-DAD analysis of EDEW was carried out to determine the presence of active constituents.ResultsQualitative analysis of various extracts of E. dracunculoides assured the existence of tannins and coumarins while presence of anthraquinones and anthocyanins was not traced in these extracts. Maximum quantity of TPC and TFC was recorded in EDEW followed by EDE. EDEW and EDE showed significant antioxidant activities with therapeutic potential against hydroxyl and phosphomolybdate radicals, β-carotene bleaching assay and in reducing of iron while moderate to low scavenging abilities were recorded for DPPH, nitric oxide and for iron chelation. During anti-inflammatory activity after 4 h of drug administration the 300 mg/kg body weight dose of EDH (68.660 ± 10.502 %) and EDE (51.384 ± 8.623 %) exhibited strong anti-inflammatory activity and reduced the carrageenan-induced paw edema in rat as compared to standard drug diclofenac sodium (78.823 ± 6.395 %). Treatment of rats with EDH (70.206 ± 5.445 %) and EDE (56.508 ± 6.363 %) after 90 min showed significant increase in percent latency time in hot plate test as compared to morphine (63.632 ± 5.449 %) treatment in rat. GC-MS analysis of EDH indicated the presence of 30 compounds predominantly of steroids and terpenoids. HPLC-DAD analysis against known standards established the presence of rutin, catechin, caffeic acid and myricetin in EDEW.ConclusionOur results suggest that presence of various polyphenolics, terpenoids and steroids render E. dracunculoides with therapeutic potential for oxidative stress and inflammation related disorders.

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Structurally diversified diterpenoids from Euphorbia dracunculoides

Cited by 21 publications

References 31 publications

Enriched Terpenes Fractions of the Latex of Euphorbia umbellata Promote Apoptosis in Leukemic Cells

Enriched Terpenes Fractions of the Latex of Euphorbia umbellata Promote Apoptosis in Leukemic Cells

Chemical Constituents from Euphorbia kansui

Studies on phytochemical, antioxidant, anti-inflammatory and analgesic activities of Euphorbia dracunculoides

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