Structural study of the frog Rana temporaria larval stomach

Histochemical and Immunohistochemical Analysis of the Stomach of Rhinella icterica (Anura, Bufonidae)

“…In R. icterica, the stomach presented four distinct layers, showing the same pattern for the digestive tube as described for its esophagus [7] and for other vertebrates [5,36,37]. Like in R. pipiens [38] (Norris, 1959) and R. aurora aurora [13], the stomach was characterized by two gastric regions: fundic and pyloric.…”

“…In tadpolesof Rana temporaria, EC cells were mainly located at the lining epithelium and occasionally found in glands [5,53]. Villaro and coworkers [53] related the fact that in Rana temporaria 5-HT and STT secreting cells are the most frequent endocrine cell types, occurring along the entire stomach, as it was observed for R. icterica.…”

“…In addition, in fundic glands, mucous cells predominated at the neck, while oxynticopeptic cells were preferentially located at the body region. For other anurans, the lining epithelium is constituted by mucous ciliated cells, as in the larval stage of Xenopus laevis [39] and in R. temporaria [5].…”

“…Regarding the specialized cells, studies of the stomach of adult frogs, as Rana esculenta [4] and Rana aurora aurora [13], as well as tadpoles of Rana temporaria [5] Gal = galactose, GalNAc = N-acetylgalactosamine, Man = mannose, Glc = glucose, and GlcNAc = N-acetylglucosamine.…”

“…Morphological studies on the anuran gastrointestinal tube have been performed by histochemical [3][4][5], ultrastructural [6], and immunohistochemical techniques [7,8], showing histological peculiarities to the stomach. These differences are more evident considering mainly the exocrine gastric glands, whose morphology varies according to the gastric region.…”