Histology and lectin-binding patterns in the digestive tract of the carnivorous larvae of the Anuran,Ceratophrys ornata

Fry, Anne E.; Kaltenbach, Jane C.

doi:10.1002/(sici)1097-4687(199907)241:1<19::aid-jmor2>3.0.co;2-4

Cited by 7 publications

(1 citation statement)

References 45 publications

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“…In anurans, mucins are produced by goblet cells found in the esophagus and the intestines, but also by epithelial cells and mucous neck cells in the stomach [43]. In R. icterica, mucous neck cells of fundic region produce neutral glycoprotein, rich in galactose residues, similar to those of R. esculenta [19], Ceratophrys ornata [44], Ceratophrys chalcides [10], and reptile Natrix natrix [45].…”

Section: Discussionmentioning

confidence: 99%

Histochemical and Immunohistochemical Analysis of the Stomach of Rhinella icterica (Anura, Bufonidae)

Machado-Santos

Pelli-Martins

Figueiredo

et al. 2014

Journal of Histology

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The stomach of Rhinella icterica was analyzed at light microscopy, employing histochemical techniques, lectin histochemistry, and immunohistochemistry for identifying enteroendocrine cells (EC). Although the stomach was composed of fundic and pyloric regions, its wall is formed by mucosa, submucosa, muscularis, and serosa. The mucosa was lined by a simple columnar mucous epithelium, supported by loose connective tissue. Several tubular, simple glands were composed of mucous neck cells, containing oxynticopeptic cells and EC cells. The mucous neck cells were rich in neutral glycoconjugates. The oxynticopeptic cells were predominant in fundic glands, exhibiting weaker alcianophilic reaction at their apical cytoplasm. Serotonin (5-HT) immunoreactive (IR) cells occurred throughout the entire stomach, preferentially located among mucous cells at upper part of the fundic glands. The muscularis mucosae, formed of smooth muscle, separated the mucosal layer from the submucosa, both of which were constituted by loose connective tissue, but without glands. Lymphoid modules occurred in the mucosa at the boundary at the stomach and the gut. In addition, the muscularis was constituted by two sublayers, the circular internal and the longitudinal external, being recovered by the connective tissue of the serosa.

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Section: Discussionmentioning

confidence: 99%

Histochemical and Immunohistochemical Analysis of the Stomach of Rhinella icterica (Anura, Bufonidae)

Machado-Santos

Pelli-Martins

Figueiredo

et al. 2014

Journal of Histology

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Apoptosis in the digestive tract of herbivorous Rana pipiens larvae and carnivorous Ceratophrys ornata larvae: An immunohistochemical study

Kaltenbach

Fry

Colpitts

2011

Journal of Morphology

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The lifespan of herbivorous Rana pipiens larvae is ∼3 months, while that of carnivorous Ceratophrys ornata larvae is only about 2 weeks. During metamorphic climax, the larval gut shortens dramatically, especially in R. pipiens, and its luminal epithelium is replaced by adult-type epithelium. To determine when programmed cell death occurs during the metamorphic restructuring of the gut, we prepared cross-sections of the stomach, small intestine, and large intestine from representative larval stages and from juvenile frogs of both species. The sections were incubated with monoclonal antibody against active caspase-3, one of the key enzymes in the apoptotic cascade. We observed apoptosis in some luminal epithelial cells in each of the three regions of the larval gastrointestinal tract of both species. However, apoptotic cells appeared earlier in larval stages of R. pipiens than C. ornata and few were seen in juvenile frogs of either species. The results demonstrate the occurrence of apoptosis in the metamorphic remodeling of the gut of both R. pipiens larvae and C. ornata larvae.

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Extreme tadpoles II: the highly derived larval anatomy of Occidozyga baluensis (Boulenger, 1896), an obligate carnivorous tadpole

et al. 2014

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Tadpoles of Occidozyga species have been reported to be carnivorous, feeding on insects and other tadpoles. We present photographic evidence for the previously undocumented larval feeding behavior in O. baluensis. Furthermore, we present a detailed anatomical description of the skull, cranial musculature, and gross gut morphology based on three-dimensional reconstructions from serial sections and lCT imagery. The cranial anatomy of larval O. baluensis is highly derived in many characters, with respect to taxa outside the genus Occidozyga, most notably the palatoquadrate and hyobranchial apparatus, that play a major role in tadpole feeding. A large larval stomach was present in the specimens examined, indicative of a macrophagous carnivorous mode of feeding. Because of the relatively small oral orifice, relatively large-sized food items found in the larval stomach, and the tunnel-like arrangement of structures that form the buccal cavity, we hypothesize that suction feeding utilizing strong negative pressure is employed by this species. Furthermore, we propose that force, rather than speed, is the main characteristic of their feeding. The unique features of the study species substantially expand the known morphospace for tadpoles, particularly among the Acosmanura (Pelobatoidea, Pelodytoidea, and Neobatrachia). Except for Microhylidae, acosmanurans previously described possess limited innovative larval morphologies. Larval carnivory has evolved convergently several times in distant anuran clades and shows structural, behavioral, and functional differences in the known examples.

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Histology and lectin-binding patterns in the digestive tract of the carnivorous larvae of the Anuran,Ceratophrys ornata

Cited by 7 publications

References 45 publications

Histochemical and Immunohistochemical Analysis of the Stomach of Rhinella icterica (Anura, Bufonidae)

Histochemical and Immunohistochemical Analysis of the Stomach of Rhinella icterica (Anura, Bufonidae)

Apoptosis in the digestive tract of herbivorous Rana pipiens larvae and carnivorous Ceratophrys ornata larvae: An immunohistochemical study

Extreme tadpoles II: the highly derived larval anatomy of Occidozyga baluensis (Boulenger, 1896), an obligate carnivorous tadpole

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