Structural study of fucoidan from sea cucumber Acaudina molpadioides: A fucoidan containing novel tetrafucose repeating unit

Yu, Long; Ge, Liang; Xue, Changhu; Chang, Yaoguang; Zhang, Cuiyu; Xu, Xiaoqi; Wang, Yanchao

doi:10.1016/j.foodchem.2013.06.079

Cited by 66 publications

(23 citation statements)

References 17 publications

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“…Fucoidan polysaccharides are a family of sulphated, fucose-rich polysaccharides uniquely produced by brown marine macroalgae (seaweeds) and certain marine invertebrates, such as sea cucumbers [ 1 , 2 ]. In general, fucoidans, also known as fucose-containing sulphated polysaccharides (FCSPs), consist of a backbone of α-L fucosyl residues linked together by (1→3) and/or (1→4)-glycoside bonds.…”

Section: Introductionmentioning

confidence: 99%

Novel Enzyme Actions for Sulphated Galactofucan Depolymerisation and a New Engineering Strategy for Molecular Stabilisation of Fucoidan Degrading Enzymes

et al. 2018

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Fucoidans from brown macroalgae have beneficial biomedical properties but their use as pharma products requires homogenous oligomeric products. In this study, the action of five recombinant microbial fucoidan degrading enzymes were evaluated on fucoidans from brown macroalgae: Sargassum mcclurei, Fucus evanescens, Fucus vesiculosus, Turbinaria ornata, Saccharina cichorioides, and Undaria pinnatifida. The enzymes included three endo-fucoidanases (EC 3.2.1.-GH 107), FcnA2, Fda1, and Fda2, and two unclassified endo-fucoglucuronomannan lyases, FdlA and FdlB. The oligosaccharide product profiles were assessed by carbohydrate-polyacrylamide gel electrophoresis and size exclusion chromatography. The recombinant enzymes FcnA2, Fda1, and Fda2 were unstable but were stabilised by truncation of the C-terminal end (removing up to 40% of the enzyme sequence). All five enzymes catalysed degradation of fucoidans containing α(1→4)-linked l-fucosyls. Fda2 also degraded S. cichorioides and U. pinnatifida fucoidans that have α(1→3)-linked l-fucosyls in their backbone. In the stabilised form, Fda1 also cleaved α(1→3) bonds. For the first time, we also show that several enzymes catalyse degradation of S. mcclurei galactofucan-fucoidan, known to contain α(1→4) and α(1→3) linked l-fucosyls and galactosyl-β(1→3) bonds in the backbone. These data enhance our understanding of fucoidan degrading enzymes and their substrate preferences and may assist development of enzyme-assisted production of defined fuco-oligosaccharides from fucoidan substrates.

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Section: Introductionmentioning

confidence: 99%

Novel Enzyme Actions for Sulphated Galactofucan Depolymerisation and a New Engineering Strategy for Molecular Stabilisation of Fucoidan Degrading Enzymes

et al. 2018

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“…Sea cucumber is a traditional tonic in China, and it contains various bioactive components, such as collagen polypeptides, polysaccharides, vanadium-binding protein and lipids [8][9][10]. Eicosapentaenoic acid-enriched phosphatidylcholine (EPA-PC), the focus of the present study, was isolated from the sea cucumber Cucumaria frondosa, which contains abundant EPA.…”

Section: Introductionmentioning

confidence: 99%

Eicosapentaenoic Acid‐Enriched Phosphatidylcholine Attenuated Hepatic Steatosis Through Regulation of Cholesterol Metabolism in Rats with Nonalcoholic Fatty Liver Disease

Liu

Shi

Tian

et al. 2016

Lipids

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Nonalcoholic fatty liver disease (NAFLD) is the most common chronic liver disease in the world. Disturbed cholesterol metabolism plays a crucial role in the development of NAFLD. The present study was conducted to evaluate the effects of EPA-PC extracted from sea cucumber on liver steatosis and cholesterol metabolism in NAFLD. Male Wistar rats were randomly divided into seven groups (normal control group, model group, lovastatin group, low- and high-dose EPA groups, and low- and high-dose EPA-PC groups). Model rats were established by administering a diet containing 1% orotic acid. To determine the possible cholesterol metabolism promoting mechanism of EPA-PC, we analyzed the transcription of key genes and transcriptional factors involved in hepatic cholesterol metabolism. EPA-PC dramatically alleviated hepatic lipid accumulation, reduced the serum TC concentration, and elevated HDLC levels in NAFLD rats. Fecal neutral cholesterol excretion was also promoted by EPA-PC administration. Additionally, EPA-PC decreased the mRNA expression of hydroxymethyl glutaric acid acyl (HMGR) and cholesterol 7α-hydroxylase (CYP7A), and increased the transcription of sterol carrying protein 2 (SCP2). Moreover, EPA-PC stimulated the transcription of peroxisome proliferators-activated receptor α (PPARα) and adenosine monophosphate activated protein kinase (AMPK) as well as its modulators, liver kinase B1 (LKB1) and Ca/calmodulin-dependent kinase kinase (CAMKK). Based on the results, the promoting effects of EPA-PC on NAFLD may be partly associated with the suppression of cholesterol synthesis via HMGR inhibition and the enhancement of fecal cholesterol excretion through increased SCP2 transcription. The underlying mechanism may involve stimulation of PPARα and AMPK.

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“…They are flexible, elongated, worm-like organisms with a leathery skin and gelatinous body. Sea cucumbers are extensively consumed in China, Japan, Korea and other Asian countries as traditional and nutritional foods and medicinal resources ( 11 ). Various components, such as triterpene glycosides, peptides, fatty acids, and SF, are present in the body walls of sea cucumbers ( 10 ).…”

Section: Introductionmentioning

confidence: 99%

Structural Effects of Sulfated-Glycoproteins from Stichopus japonicus on the Nitric Oxide Secretion Ability of RAW 264.7 Cells

Cao

Lee

You

2014

JFN

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The effect of various levels of proteins, sulfates, and molecular weight (Mw) of a sulfated-glycoprotein (NF3) from a sea cucumber, Stichopus japonicus, on nitric oxide (NO) releasing capacity from RAW 264.7 cells was investigated. The NF3 derivatives had various amounts of proteins (4.8~11.2%) and sulfates (6.8~25.2%) as well as different Mw (640.3×103~109.2×103 g/mol). NF3 was able to stimulate RAW 264.7 cells to release NO with lower protein contents, indicating that the protein moiety was not an important factor to stimulate macrophages. On the other hand, the NO inducing capacity was significantly reduced with decreased levels of sulfates and Mw, implying that sulfates and Mw played a pivotal role in activating RAW 264.7 cells. It was not clear why sulfates and a certain range of Mw were essential for stimulating macrophages. It appeared that certain levels of sulfates and Mw of sulfated-glycoproteins were required to bind to the surface receptors on RAW 264.7 cells.

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Structural study of fucoidan from sea cucumber Acaudina molpadioides: A fucoidan containing novel tetrafucose repeating unit

Cited by 66 publications

References 17 publications

Novel Enzyme Actions for Sulphated Galactofucan Depolymerisation and a New Engineering Strategy for Molecular Stabilisation of Fucoidan Degrading Enzymes

Novel Enzyme Actions for Sulphated Galactofucan Depolymerisation and a New Engineering Strategy for Molecular Stabilisation of Fucoidan Degrading Enzymes

Eicosapentaenoic Acid‐Enriched Phosphatidylcholine Attenuated Hepatic Steatosis Through Regulation of Cholesterol Metabolism in Rats with Nonalcoholic Fatty Liver Disease

Structural Effects of Sulfated-Glycoproteins from Stichopus japonicus on the Nitric Oxide Secretion Ability of RAW 264.7 Cells

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