“…The micro-environment of the micelles may contribute to altered kinetic properties. The overall liquid-liquid extraction process by reverse micelles is conducted in two fundamental steps: a forward extraction, by which the target protein or the contaminants are transferred from an aqueous solution to a reverse micellar organic phase, and a back extraction, by which the biomolecules are released from the reversed micelles and transferred to a fresh aqueous phase, so that it can be subsequently recovered [3,4]. The separation of the target protein also can be achieved if, during the extration, it is retained in the remaining aqueous phase (API), and the contaminants are transferred into micelles, or vice versa.…”