1993
DOI: 10.1021/la00035a022
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Structural study of AOT reverse micelles containing native and modified .alpha.-chymotrypsin

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Cited by 44 publications
(40 citation statements)
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“…Assays were conducted randomly. The range of values are in agreement with those reported by several authors [2][3][4][5].…”
Section: Experimental Design and Optimization 281 Factorial Designsupporting
confidence: 93%
See 1 more Smart Citation
“…Assays were conducted randomly. The range of values are in agreement with those reported by several authors [2][3][4][5].…”
Section: Experimental Design and Optimization 281 Factorial Designsupporting
confidence: 93%
“…The micro-environment of the micelles may contribute to altered kinetic properties. The overall liquid-liquid extraction process by reverse micelles is conducted in two fundamental steps: a forward extraction, by which the target protein or the contaminants are transferred from an aqueous solution to a reverse micellar organic phase, and a back extraction, by which the biomolecules are released from the reversed micelles and transferred to a fresh aqueous phase, so that it can be subsequently recovered [3,4]. The separation of the target protein also can be achieved if, during the extration, it is retained in the remaining aqueous phase (API), and the contaminants are transferred into micelles, or vice versa.…”
Section: Introductionmentioning
confidence: 99%
“…When all micelles have the same spherical core-shell structure but they differ in their size, the polydisperse form factor P s (h) can be obtained from P s (h) by assuming a Gaussian (normal) size distribution of the core radius [23] with σ as the root-mean-square deviation from the mean water core radius R c , and a constant shell thickness:…”
Section: Modeling Of the Saxs Intensitiesmentioning
confidence: 99%
“…This model has been applied successfully to various systems having short-ranged attractive forces, including sterically stabilized dispersions (31), surfactant-based microemulsions (32), particles flocculated/gelled by bridging polymers (33,34), aggregating casein micelles (11,35), and flocculated caseincoated emulsion droplets (36). The sticky hard-sphere system has a critical temperature defined by τ c = 0.098 and a critical volume fraction φ c = 0.12.…”
Section: Reversible Aggregation and Gelationmentioning
confidence: 99%