Structural studies on colanic acid, the common exopolysaccharide found in the Enterobacteriaceae, by partial acid hydrolysis. Oligosaccharides from colanic acid

Sutherland, Ian W.

doi:10.1042/bj1150935

Cited by 91 publications

(57 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…EGR63372) motif (E value, 4.69eϪ19) and homology to Escherichia and Shigella putative colanic acid biosynthesis proteins from residues 264 to 446. Salmonella strains are known to produce colanic acid-containing exopolysaccharides under specific environmental conditions (23,24,(65)(66)(67)71). The N terminus shows sequence similarity to both gp43 (GenBank accession no.…”

Section: Resultsmentioning

confidence: 99%

Genomic and Proteomic Characterization of the Broad-Host-Range Salmonella Phage PVP-SE1: Creation of a New Phage Genus

Santos

Kropinski

Ceyssens

et al. 2011

J Virol

View full text Add to dashboard Cite

(Bacterio)phage PVP-SE1, isolated from a German wastewater plant, presents a high potential value as a biocontrol agent and as a diagnostic tool, even compared to the well-studied typing phage Felix 01, due to its broad lytic spectrum against different Salmonella strains. Sequence analysis of its genome (145,964 bp) shows it to be terminally redundant and circularly permuted. Its G؉C content, 45.6 mol%, is lower than that of its hosts (50 to 54 mol%). We found a total of 244 open reading frames (ORFs), representing 91.6% of the coding capacity of the genome. Approximately 46% of encoded proteins are unique to this phage, and 22.1% of the proteins could be functionally assigned. This myovirus encodes a large number of tRNAs (n ‫؍‬ 24), reflecting its lytic capacity and evolution through different hosts. Tandem mass spectrometric analysis using electron spray ionization revealed 25 structural proteins as part of the mature phage particle. The genome sequence was found to share homology with 140 proteins of the Escherichia coli bacteriophage rV5. Both phages are unrelated to any other known virus, which suggests that an "rV5-like virus" genus should be created within the Myoviridae to contain these two phages.

show abstract

Section: Resultsmentioning

confidence: 99%

Genomic and Proteomic Characterization of the Broad-Host-Range Salmonella Phage PVP-SE1: Creation of a New Phage Genus

Santos

Kropinski

Ceyssens

et al. 2011

J Virol

View full text Add to dashboard Cite

show abstract

“…Pyruvic acid was determined as the 2,4-dinitrophenylhydrazone derivative by the method of Sutherland [7] with a slight modification. Glycolipids containing 5 -10 pg pyruvic acid were hydrolyzed with 0.2 ml 1 M HCl at 100°C for 3 h and the hydrolysate was mixed with 0.1 ml 0.5% (mass/vol.)…”

Section: Analysis Of Chemical Compositionmentioning

confidence: 99%

Novel phosphonoglycosphingolipids containing pyruvylated galactose from the nervous system of Aplysia kurodai

Araki

Abe

Satake

et al. 1991

European Journal of Biochemistry

View full text Add to dashboard Cite

We have now isolated two other pyruvylated galactose-containing phosphonoglycosphingolipids, named FGL-V and FGL-IIa, from the nervous tissue of Aplysiu, and characterized them as [3,4-O-(S-l -carboxyethy1idene)lGalPl + 3GalNAcal --f 3[6'-0-(2-arninoethylphosphonyl)Galal + 21 (2-aminoethylphosphonyl + 6) GalBl + 4GlcP1 + 1 ceramide and [3,4,O-(S-1 -carboxyethylidene)]Gal/?l + 3GalNAc a1 + 3[6'-0-(2-aminoethylphosphony1)Galal 4 2lGalPI + 4Glcfi11 + ceramide, respectively.Their major aliphatic components are palmitic acid, octadeca-4-sphingenine and anteisononadeca-4-sphingenine.Thus, the nervous system of Aplysiu contains several pyruvylated phosphonoglycolipids.The marine mollusc Aplysia has been used successfully in studies on the cellular and molecular processes of neuronal functions.We have found a new family of phosphonoglycosphingolipids in the tissues of Aplysiu. Of these, six predominant glycolipids, tentatively named FGL-I, FGL-IIa, FGL-lib, FGL-V, F-9 and F-21, were found only in the nervous systemWe have reported the isolation of FGL-IIb and its characterization as [3,4-0-(1-carboxyethylidene)]Gal~1 + 3GalNAcal + 3(Fucal -+ 2)(2-aminoethylphosphonyl + 6). Antiserum raised against FGL-IIb reacted not only with FGL-IIb, but also with FGL-I, FGL-IIa, FGL-V and F-9 on thin-layer immunostaining [2]. These glycolipids seemed to have a pyruvic acid residue linked to galactose at their nonreducing end, because the immunochemical study suggested that the carboxyl group of pyruvic acid linked to the terminal galactose of FGL-IIb was an essential epitope for the binding of the antibody [3].In the present paper, we determined the structures of two related phosphonoglycosphingolipids, FGL-V and FGL-IIa, isolated from the nervous tissue of Aplysiu. EXPERIMENTAL PROCEDURES Fractionation and purijkation of glycolipids FGL-V and FGL-IIaTotal glycolipids (927 mg) were prepared from an acetone powder (100 g) of the nervous tissue (1 kg) of Aplysiu as Correspondence to S. Araki, Department of Neurochemistry, Brain Research Institute, Niigata University, Asahimachi 1, Niigata, Japan 951Abbreviations. 2-AEP, 2-aminoethylphosphonate; FAB-MS, fastatom-bombardment mass spectrometry; COSY, chemical-shift-correlated spectroscopy. described previously [4]. FGL-V and FGL-IIa were purified further by successive chromatography on an Iatrobeads column. The total glycolipid fraction (1 50 mg/run) was dissolved in 2 ml chloroform/methanol/water (60: 35 : 8, by vol.) and applied to a column of Iatrobeads (6RS-8060, Iatron Lab., Tokyo; 1.6 x 36.5 cm). Glycolipids were eluted with a discontinuous gradient of chloroform/methanol/water as described previously [4] and monitored by high-performance TLC using 1-propanol/concentrated ammonium hydroxide/ water (75 : 5 : 25, by vol.). Three glycolipid fractions were obtained by column chromatography. The third one, containing FGL-V, was re-chromatographed on another Iatrobeads column (1 .O x 45 cm) using 300 ml of a linear gradient of 1-propanol/concentrated ammonium hydroxidelwater (75 : 5 : 20 ...

show abstract

“…Apart from some deviation due to traces of rhamnose, mannose and galacturonic acid all of these EPSs have the same composition. The sugar/ substituent compositions of the nine EPSs described above are the same as the composition found for colanic acid [16,40], commonly produced by several members of the family Enterobacteriaceae [16]. The EPS produced by Methylobacterium sp.…”

Section: Characterisation Of the Polysaccharidesmentioning

confidence: 49%

Colanic acid is an exopolysaccharide common to many enterobacteria isolated from paper-machine slimes

Rättö

Verhoef

Suihko

et al. 2006

J IND MICROBIOL BIOTECHNOL

View full text Add to dashboard Cite

In this study, polysaccharide-producing bacteria were isolated from slimes collected from two Finnish and one Spanish paper mill and the exopolysaccharides (EPSs) produced by 18 isolates were characterised. Most of the isolates, selected on the bases of slimy colony morphology, were members of the family Enterobacteriaceae most frequently belonging to the genera Enterobacter and Klebsiella including Raoultella. All of the EPSs analysed showed the presence of charged groups in the form of uronic acid or pyruvate revealing the polyanionic nature of these polysaccharides. Further results of the carbohydrate analysis showed that the EPS produced by nine of the enterobacteria was colanic acid.

show abstract

Structural studies on colanic acid, the common exopolysaccharide found in the Enterobacteriaceae, by partial acid hydrolysis. Oligosaccharides from colanic acid

Cited by 91 publications

References 25 publications

Genomic and Proteomic Characterization of the Broad-Host-Range Salmonella Phage PVP-SE1: Creation of a New Phage Genus

Genomic and Proteomic Characterization of the Broad-Host-Range Salmonella Phage PVP-SE1: Creation of a New Phage Genus

Novel phosphonoglycosphingolipids containing pyruvylated galactose from the nervous system of Aplysia kurodai

Colanic acid is an exopolysaccharide common to many enterobacteria isolated from paper-machine slimes

Contact Info

Product

Resources

About