Genomic and Proteomic Characterization of the Broad-Host-Range Salmonella Phage PVP-SE1: Creation of a New Phage Genus

Abstract

(Bacterio)phage PVP-SE1, isolated from a German wastewater plant, presents a high potential value as a biocontrol agent and as a diagnostic tool, even compared to the well-studied typing phage Felix 01, due to its broad lytic spectrum against different Salmonella strains. Sequence analysis of its genome (145,964 bp) shows it to be terminally redundant and circularly permuted. Its G؉C content, 45.6 mol%, is lower than that of its hosts (50 to 54 mol%). We found a total of 244 open reading frames (ORFs), representing 91.6% of the coding capacity of the genome. Approximately 46% of encoded proteins are unique to this phage, and 22.1% of the proteins could be functionally assigned. This myovirus encodes a large number of tRNAs (n ‫؍‬ 24), reflecting its lytic capacity and evolution through different hosts. Tandem mass spectrometric analysis using electron spray ionization revealed 25 structural proteins as part of the mature phage particle. The genome sequence was found to share homology with 140 proteins of the Escherichia coli bacteriophage rV5. Both phages are unrelated to any other known virus, which suggests that an "rV5-like virus" genus should be created within the Myoviridae to contain these two phages.

Testing hypotheses for the presence of tRNA genes in mycobacteriophage genomes

“…[24][25][26][27] In phage PVP-SE1, only 11 of the codons associated with its 24 tRNA genes were present at a frequency higher than in its Salmonella hosts. 28 In the 5 Acinetobacter phages studied by Jin et al, an association between the presence of tRNA genes and codon usage could only be observed for one Acinetobacter phage, ZZ1, and for only 5 out of the 8 tRNA genes. 4 In the Acinetobacter Acibel004 phage, 16 out of its 22 tRNAs are found in higher frequency in the phage's genome than in its host A. baumannii.…”

A<i>β</i>-Like Peptide Displayed on Bacteriophage T7 Catalyzes Chromate and Uranyl Reduction

“…Further testing is required to determine whether the engineered bacteriophage can be used for field-deployment. Although many different types and phage strains have been isolated from the environment, and while the application of these bacteriophage for the purposes of gene typing and water quality indication have been well studied [41][42][43], the application of isolated bacteriophage or engineered bacteirophage for bioremediation has not yet explored. Therefore the study reported here offers initial data and analysis useful to the applications of bacteriophage for uranium bioremedia tions at Cr and U contaminated sites such as those managed by the US Department of Energy.…”

Phages as Therapeutic Tools to Control Major Foodborne Pathogens: Campylobacter and Salmonella

“…Felix 01 is characterized by its broad lytic spectrum among Salmonella and has been used as a diagnostic tool in the identification of Salmonella. Recently, a phage with a broader host range than Felix 01 has been described which presents great potential not only as a therapeutic agent but also as a diagnostic tool Santos et al, 2011;Sillankorva et al, 2010) (Figure 2). Fig.…”