Structural studies of the O-chain of the phenol-phase soluble lipopolysaccharide from <i>Haemophilus pleuropneumoniae</i> serotype 2

Altman, Eleonora; Brisson, Jean‐Robert; Bundle, David R.; Perry, Malcolm B.

doi:10.1139/o87-114

Cited by 17 publications

(4 citation statements)

References 26 publications

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“…The LPS structure of A. pleuropneumoniae serotype 2 extracted by phenol-water extraction was smooth-type LPS including a lipid A moiety [1,14]. It was reported that only very small antigenic variations in the LPS components should also be observed in B. bronchiseptica [3].…”

Section: Discussionmentioning

confidence: 99%

Biological Activities of Lipopolysaccharides Extracted from Porcine Vaccine Strains.

Ogikubo

Norimatsu

Kojima

et al. 1999

The Journal of Veterinary Medical Science

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ABSTRACT. Lipopolysaccharides (LPSs) were purified from Actinobacillus pleuropneumoniae serotype 2, Bordetella bronchiseptica and Haemophilus parasuis serotype 5, which were used for vaccine production in Japan, by the phenol-water procedure. In SDS-PAGE analysis, A. pleuropneumoniae LPS, as well as Escherichia coli LPS, demonstrated a typical ladder profile of a smooth-type LPS. On the other hand, B. bronchiseptica and H. parasuis LPSs lacked the ladder profiles. It was found that the biological activity of these LPSs was comparable to those of E. coli LPS in terms of activation of the clotting enzyme of Limulus amoebocyte lysate, mitogenic activity of mouse spleen cells, stimulation of TNF-α and nitric oxide production, but IL-6 production could hardly be observed in any LPS.-KEY WORDS: Actinobacillus pleuropneumoniae, biological activity, Bordetella bronchiseptica, Haemophilus parasuis, lipopolysaccharide.

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Section: Discussionmentioning

confidence: 99%

Biological Activities of Lipopolysaccharides Extracted from Porcine Vaccine Strains.

Ogikubo

Norimatsu

Kojima

et al. 1999

The Journal of Veterinary Medical Science

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“…The core oligosaccharide of serotypes 1-7 contains glucose, heptose and 3-deoxy-D-manno-octulosonic acid (Byrd and Kadis, 1989). Differences were observed in the composition of the core oligosaccharide of serotypes 1 and 2 of A. pleuropneumoniae (Altman et al, 1986(Altman et al, , 1987. The lipid A component consists predominantly of C14:0, C16:0 and 3-hydroxy C14:0 fatty acids and make up about 9.2% of the total LPS.…”

Section: Lipopolysaccharidesmentioning

confidence: 97%

Actinobacillus pleuropneumoniaesurface polysaccharides: their role in diagnosis and immunogenicity

Dubreuil

Jacques

Mittal

et al. 2000

Anim. Health. Res. Rev.

111

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Actinobacillus pleuropneumoniae is an important pig pathogen that is responsible for swine pleuropneumonia, a highly contagious respiratory infection. Knowledge of the importance, composition and structural determination of the major antigens involved in virulence provides crucial information that could lead to the development of a rationale for the production of specific serodiagnostic tools as well as vaccine development. Thus, efforts have been devoted to study mainly A. pleuropneumoniae virulence determinants with special emphasis on the Apx toxins (for A. pleuropneumoniae RTX toxins). In comparison, little attention has been given to the surface polysaccharides, which include capsular polysaccharides (CPS) and cell-wall lipopolysaccharides (LPS). Here, we review current knowledge on CPS and LPS of A. pleuropneumoniae used as diagnostic tools to monitor the infection and as immunogens for inclusion in vaccine preparations for animal protection.

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“…Our structural studies of the antigens of the different serotypes of A. pleuropneumoniae have revealed the association of a unique lipopolysaccharide with each serotype, the differences residing in the structure of their respective 0-polysaccharide components (6)(7)(8)(9). This paper records the structural analysis of the 0-polysaccharide component of the lipopolysaccharide produced by A. pleuropneumoniae serotype 6 and relates its structure and observed serological cross reactivity (10) with that of the lipopolysaccharide produced by A. pleuropneumoniae serotype 3.…”

Section: (4)mentioning

confidence: 99%

Structure of the lipopolysaccharide O-antigen of Actinobacillus (Haemophilus) pleuropneumoniae serotype 6 (ATCC 33590)

Altman¹,

Brisson²,

Perry³

1989

Can. J. Chem.

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. Can. J. Chem. 67, 703 (1989). One-and two-dimensional high resolution 'H and I3C nuclear magnetic resonance spectroscopy at 500 MHz and 125 MHz together with methylation and partial hydrolytic degradation methods were used to determine the structure of the antigenic 0-polysaccharide produced by Actinobacillus (Haemophilus) pleuropneumoniae serotype 6 (ATCC 33590). The Opolysaccharide was shown to be a high molecular weight unbranched linear polymer of repeating pentasaccharide units having the structure: +3)-a-~-Glcp-(1+2)-P-~-Galf-(1+6)-a-~-Glcp-(1+6)-P-~-Glcp-(l+3)-P-~-Galf-(l+.

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Structural studies of the O-chain of the phenol-phase soluble lipopolysaccharide from Haemophilus pleuropneumoniae serotype 2

Cited by 17 publications

References 26 publications

Biological Activities of Lipopolysaccharides Extracted from Porcine Vaccine Strains.

Biological Activities of Lipopolysaccharides Extracted from Porcine Vaccine Strains.

Actinobacillus pleuropneumoniaesurface polysaccharides: their role in diagnosis and immunogenicity

Structure of the lipopolysaccharide O-antigen of Actinobacillus (Haemophilus) pleuropneumoniae serotype 6 (ATCC 33590)

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