Structural studies of the lipopolysaccharide from Haemophilus parainfluenzae strain 20

Vitiazeva, Varvara; Twelkmeyer, Brigitte; Young, R. P.; Hood, Derek W.; Schweda, Elke K. H.

doi:10.1016/j.carres.2011.07.006

Cited by 6 publications

(10 citation statements)

References 111 publications

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“…an enzyme that connects two sugars via a phosphodiester linkage, as seen in the strain 20 O-unit structure). The presence of the three transferase genes and the lack of sialic acid biosynthesis/transfer genes in the strain 20 OAg locus is consistent with the observed trisaccharide O-unit structure, which contains FucNAc4N, glucose phosphate and GalNAc (Vitiazeva et al, 2011). …”

Section: Resultssupporting

confidence: 80%

“…This resulted in the complete loss of OAg from both strain 20 (Vitiazeva et al, 2011) and strain 15 (Fig. 3A), demonstrating that the respective loci are required for OAg formation as predicted.…”

Section: Resultssupporting

confidence: 67%

“…We have recently shown that deleting wcfS in H. parainfluenzae strain 20 results in both the loss of a ladder pattern on the SDS-PAGE LPS profile and the loss of detectable OAg using structural analysis, confirming the involvement of this gene in OAg production (Vitiazeva et al, 2011). …”

Section: Resultsmentioning

confidence: 81%

“…This indicated that several strains may be capable of decorating their LPS with at least one of the same sugars as strain T3T1, namely FucNAc4N. In parallel with the determination of the OAg structure from one of these strains, H. parainfluenzae 20 (Vitiazeva et al, 2011), we sequenced and analysed its full OAg locus. This strain was chosen for analysis due to its high level of OAg expression and because its short OAg locus indicated a different genetic composition to that of T3T1.…”

Section: Resultsmentioning

confidence: 99%

“…We recently showed that in contrast to the closely related species Haemophilus influenzae , H. parainfluenzae does not phase vary the expression of its core LPS components by the tetranucleotide repeat mediated slippage of LPS biosynthesis genes (Young and Hood, 2013) and at least one strain expresses polymeric OAg (Vitiazeva et al, 2011). The latter observation concurs with the findings of Roberts and colleagues (1986) that some (8/25) H. parainfluenzae isolates give ladder-like LPS profiles using silver-stained SDS-PAGE, suggestive of molecules containing OAgs of different chain lengths.…”

Section: Introductionmentioning

confidence: 99%

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Haemophilus parainfluenzae expresses diverse lipopolysaccharide O-antigens using ABC transporter and Wzy polymerase-dependent mechanisms

Young

Twelkmeyer

Vitiazeva

et al. 2013

International Journal of Medical Microbiology

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Lipopolysaccharide O-antigens are the basis of serotyping schemes for Gram negative bacteria and help to determine the nature of host–bacterial interactions. Haemophilus parainfluenzae is a normal commensal of humans but is also an occasional pathogen. The prevalence, diversity and biosynthesis of O-antigens were investigated in this species for the first time. 18/18 commensal H. parainfluenzae isolates contain a O-antigen biosynthesis gene cluster flanked by glnA and pepB, the same position as the hmg locus for tetrasaccharide biosynthesis in Haemophilus influenzae. The O-antigen loci show diverse restriction digest patterns but fall into two main groups: (1) those encoding enzymes for the synthesis and transfer of FucNAc4N in addition to the Wzy-dependent mechanism of O-antigen synthesis and transport and (2) those encoding galactofuranose synthesis/transfer enzymes and an ABC transporter. The other glycosyltransferase genes differ between isolates. Three H. parainfluenzae isolates fell outside these groups and are predicted to synthesise O-antigens containing ribitol phosphate or deoxytalose. Isolates using the ABC transporter system encode a putative O-antigen ligase, required for the synthesis of O-antigen-containing LPS glycoforms, at a separate genomic location. The presence of an O-antigen contributes significantly to H. parainfluenzae resistance to the killing effect of human serum in vitro. The discovery of O-antigens in H. parainfluenzae is striking, as its close relative H. influenzae lacks this cell surface component.

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Section: Resultssupporting

confidence: 80%

Section: Resultssupporting

confidence: 67%

Section: Resultsmentioning

confidence: 81%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Haemophilus parainfluenzae expresses diverse lipopolysaccharide O-antigens using ABC transporter and Wzy polymerase-dependent mechanisms

Young

Twelkmeyer

Vitiazeva

et al. 2013

International Journal of Medical Microbiology

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Haemophilus parainfluenzae has a limited core lipopolysaccharide repertoire with no phase variation

Young

Hood

2012

Glycoconj J

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Cell surface lipopolysaccharide (LPS) is a well characterized virulence determinant for the human pathogen Haemophilus influenzae, so an investigation of LPS in the less pathogenic Haemophilus parainfluenzae could yield important insights. Using a panel of 18 commensal H. parainfluenzae isolates we demonstrate that the set of genes for inner core LPS biosynthesis largely resembles that of H. influenzae, with an additional heptosyltransferase I gene similar to waaC from Pasteurella multocida. Inner core LPS structure is therefore likely to be largely conserved across the two Haemophilus species. Outer core LPS biosynthetic genes are much less prevalent in H. parainfluenzae, although homologues of the H. influenzae LPS genes lpsB, non-phase variable lic2A and lgtC, and losA1, losB1 and lic2C are found in certain isolates. Immunoblotting using antibodies directed against selected LPS epitopes was consistent with these data. We found no evidence for tetranucleotide repeat-mediated phase variation in H. parainfluenzae. Phosphocholine, a phase variable H. influenzae LPS epitope that has been implicated in disease, was absent in H. parainfluenzae LPS as were the respective (lic1) biosynthetic genes. The introduction of the lic1 genes into H. parainfluenzae led to the phase variable incorporation of phosphocholine into its LPS. Differences in LPS structure between Haemophilus species could affect interactions at the bacterial-host interface and therefore the pathogenic potential of these bacteria.

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Expression of a new disialyllacto structure in the lipopolysaccharide of non-typeable Haemophilus influenzae

Twelkmeyer

Burström

et al. 2011

Carbohydrate Research

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Structural studies of the lipopolysaccharide from Haemophilus parainfluenzae strain 20

Cited by 6 publications

References 111 publications

Haemophilus parainfluenzae expresses diverse lipopolysaccharide O-antigens using ABC transporter and Wzy polymerase-dependent mechanisms

Haemophilus parainfluenzae expresses diverse lipopolysaccharide O-antigens using ABC transporter and Wzy polymerase-dependent mechanisms

Haemophilus parainfluenzae has a limited core lipopolysaccharide repertoire with no phase variation

Expression of a new disialyllacto structure in the lipopolysaccharide of non-typeable Haemophilus influenzae

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