Haemophilus parainfluenzae has a limited core lipopolysaccharide repertoire with no phase variation

Young, R. P.; Hood, Derek W.

doi:10.1007/s10719-012-9455-5

Cited by 7 publications

(6 citation statements)

References 54 publications

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“…In agreement with the reported increase in bacterial translocation in cirrhotic patients, B. dentium , an opportunistic pathogen that mainly inhabits the oral cavity (Xu et al, 2012), and H. parainfluenzae residing primarily in the human upper respiratory tract (Young and Hood, 2013) increased during disease progression. Surprisingly, L. salivarius , and S. salivarius levels also increased during disease progression in the current study.…”

Section: Discussionsupporting

confidence: 88%

Disorganized Gut Microbiome Contributed to Liver Cirrhosis Progression: A Meta-Omics-Based Study

et al. 2018

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Early detection and effective interventions for liver cirrhosis (LC) remain an urgent unmet clinical need. Inspired from intestinal disorders in LC patients, we investigated the associations between gut microbiome and disease progression based on a raw metagenomic dataset of 47 healthy controls, 49 compensated, and 46 decompensated LC patients from our previous study, and a metabolomic dataset of urine samples from the same controls/patients using ultra-performance liquid chromatography/mass spectrophotometry system. It was found that the combination and relative abundance of gut microbiome, the inter-microbiome regulatory networks, and the microbiome-host correlation patterns varied during disease progression. The significant reduction of bacteria involved in fermentation of plant cell wall polysaccharides and resistant starch (such as Alistipes sp. HG5, Clostridium thermocellum) contributed to the reduced supply of energy sources, the disorganized self-feeding and cross-feeding networks and the thriving of some opportunistic pathogens in genus Veillonella. The marked decrease of butyrate-producing bacteria and increase of Ruminococcus gnavus implicated in degradation of elements from the mucus layer provided an explanation for the impaired intestinal barrier function and systematic inflammation in LC patients. Our results pave the way for further developments in early detection and intervention of LC targeting on gut microbiome.

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Section: Discussionsupporting

confidence: 88%

Disorganized Gut Microbiome Contributed to Liver Cirrhosis Progression: A Meta-Omics-Based Study

et al. 2018

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“…Amongst the H. parainfluenzae strains tested we found no correlation between the type of OAg locus and the presence of particular H. influenzae -like outer core LPS biosynthesis genes that we had previously identified (Young and Hood, 2013) such as lpsB , losB1 and lic2C . This emphasises the high degree of genetic exchange that must occur in H. parainfluenzae , particularly in the genes required for OAg synthesis.…”

Section: Discussioncontrasting

confidence: 58%

“…We recently showed that in contrast to the closely related species Haemophilus influenzae , H. parainfluenzae does not phase vary the expression of its core LPS components by the tetranucleotide repeat mediated slippage of LPS biosynthesis genes (Young and Hood, 2013) and at least one strain expresses polymeric OAg (Vitiazeva et al, 2011). The latter observation concurs with the findings of Roberts and colleagues (1986) that some (8/25) H. parainfluenzae isolates give ladder-like LPS profiles using silver-stained SDS-PAGE, suggestive of molecules containing OAgs of different chain lengths.…”

Section: Introductionmentioning

confidence: 99%

Haemophilus parainfluenzae expresses diverse lipopolysaccharide O-antigens using ABC transporter and Wzy polymerase-dependent mechanisms

Young

Twelkmeyer

Vitiazeva

et al. 2013

International Journal of Medical Microbiology

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Lipopolysaccharide O-antigens are the basis of serotyping schemes for Gram negative bacteria and help to determine the nature of host–bacterial interactions. Haemophilus parainfluenzae is a normal commensal of humans but is also an occasional pathogen. The prevalence, diversity and biosynthesis of O-antigens were investigated in this species for the first time. 18/18 commensal H. parainfluenzae isolates contain a O-antigen biosynthesis gene cluster flanked by glnA and pepB, the same position as the hmg locus for tetrasaccharide biosynthesis in Haemophilus influenzae. The O-antigen loci show diverse restriction digest patterns but fall into two main groups: (1) those encoding enzymes for the synthesis and transfer of FucNAc4N in addition to the Wzy-dependent mechanism of O-antigen synthesis and transport and (2) those encoding galactofuranose synthesis/transfer enzymes and an ABC transporter. The other glycosyltransferase genes differ between isolates. Three H. parainfluenzae isolates fell outside these groups and are predicted to synthesise O-antigens containing ribitol phosphate or deoxytalose. Isolates using the ABC transporter system encode a putative O-antigen ligase, required for the synthesis of O-antigen-containing LPS glycoforms, at a separate genomic location. The presence of an O-antigen contributes significantly to H. parainfluenzae resistance to the killing effect of human serum in vitro. The discovery of O-antigens in H. parainfluenzae is striking, as its close relative H. influenzae lacks this cell surface component.

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“…Gram-negative bacterium that normally resides within the human nasopharynx and periodontal spaces, where its carriage is usually without significant symptoms (19). This species is differentiated from the closely related Haemophilus influenzae by the lack of a strict requirement for hemin and differences in its surface glycolipid populations (20)(21)(22)(23). Notably, many of these surface factors lacking in H. parainfluenzae are those that we have previously shown to be important determinants of biofilm formation and maturation for H. influenzae (14,(24)(25)(26).…”

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confidence: 90%

Haemophilus parainfluenzae Strain ATCC 33392 Forms BiofilmsIn Vitroand during Experimental Otitis Media Infections

Pang

Swords

2017

Infect Immun

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is a nutritionally fastidious, Gram-negative bacterium with an oropharyngeal/nasopharyngeal carriage niche that is associated with a range of opportunistic infections, including infectious endocarditis and otitis media (OM). These infections are often chronic/recurrent in nature and typically involve bacterial persistence within biofilm communities that are highly resistant to host clearance. This study addresses the primary hypothesis that forms biofilm communities that are important determinants of persistence The results from biofilm studies confirmed that formed biofilm communities within which the polymeric matrix was mainly composed of extracellular DNA and proteins. Using a chinchilla OM infection model, we demonstrated that formed surface-associated biofilm communities containing bacterial and host components that included neutrophil extracellular trap (NET) structures and that the bacteria mainly persisted in these biofilm communities. We also used this model to examine the possible interaction between and its close relative , which is also commonly carried within the same host environments and can cause OM. The results showed that coinfection with promoted clearance of from biofilm communities during OM infection. The underlying mechanisms for bacterial persistence and biofilm formation by and knowledge about the survival defects of during coinfection with are topics for future work.

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Haemophilus parainfluenzae has a limited core lipopolysaccharide repertoire with no phase variation

Cited by 7 publications

References 54 publications

Disorganized Gut Microbiome Contributed to Liver Cirrhosis Progression: A Meta-Omics-Based Study

Disorganized Gut Microbiome Contributed to Liver Cirrhosis Progression: A Meta-Omics-Based Study

Haemophilus parainfluenzae expresses diverse lipopolysaccharide O-antigens using ABC transporter and Wzy polymerase-dependent mechanisms

Haemophilus parainfluenzae Strain ATCC 33392 Forms BiofilmsIn Vitroand during Experimental Otitis Media Infections

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