Structural Studies of Component of Lysoamidase Bacteriolytic Complex from Lysobacter sp. XL1

Tishchenko, Svetlana; Габдулхаков, А.Г.; Melnik, Bogdan S.; Kudryakova, Irina V.; Latypov, O. R.; Vasilyeva, Natalya Leontyevskaya; Leontievsky, A. A.

doi:10.1007/s10930-015-9645-7

Cited by 10 publications

(2 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…oxysporum , the ginseng root rot pathogen, was cultured on PDA medium for 7 days. Fungal spores were gently washed from the plates with sterile water and the obtained spore suspension was adjusted to 100 spores/ml [ 40 ]. The fungal conidial suspension was then evenly sprayed on the root discs laid out on a tray and left to air dry for 15 minutes.…”

Section: Methodsmentioning

confidence: 99%

The potential of novel bacterial isolates from healthy ginseng for the control of ginseng root rot disease (Fusarium oxysporum)

Yan²,

Miao³

et al. 2022

PLoS ONE

View full text Add to dashboard Cite

Ginseng root rot caused by Fusarium oxysporum is serious disease that impacts ginseng production. In the present study, 145 strains of bacteria were isolated from the rhizosphere soil of healthy ginseng plants. Three strains with inhibitory activity against Fusarium oxysporum (accession number AF077393) were identified using the dual culture tests and designated as YN-42(L), YN-43(L), and YN-59(L). Morphological, physiological, biochemical, 16S rRNA gene sequencing and phylogenetic analyses were used to identify the strains as Bacillus subtilis [YN-42(L)] (accession number ON545980), Delftia acidovorans [YN-43(L)] (accession number ON545981), and Bacillus polymyxae [YN-59(L)] (accession number ON545982). All three isolates effectively inhibited the growth of Fusarium oxysporum in vitro and the antagonistic mechanism used by the three strains involved the secretion of multiple bioactive metabolites responsible for the hydrolysis of the fungal cell wall. All three biocontrol bacteria produce indoleacetic acid, which has a beneficial effect on plant growth. From our findings, all three antagonistic strains can be excellent candidates for ginseng root rot caused by the pathogenic fungus Fusarium oxysporum. These bacteria have laid the foundation for the biological control of ginseng root rot and for further research on the field control of ginseng pathogens.

show abstract

Section: Methodsmentioning

confidence: 99%

The potential of novel bacterial isolates from healthy ginseng for the control of ginseng root rot disease (Fusarium oxysporum)

Yan²,

Miao³

et al. 2022

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…Our main works investigate such enzymes in Lysobacter sp. XL1 [ 11 , 12 , 13 , 14 , 15 , 16 , 17 ]. Our recent research has been related to the study of antimicrobial potential in L. capsici VKM B-2533 T [ 6 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

The First Homologous Expression System for the β-Lytic Protease of Lysobacter capsici VKM B-2533T, a Promising Antimicrobial Agent

Kudryakova

Afoshin

Leontyevskaya

et al. 2022

IJMS

Self Cite

View full text Add to dashboard Cite

A successful homologous expression system based on Lysobacter capsici VKM B-2533T and the plasmid pBBR1-MCS5 was first developed for a promising bacteriolytic enzyme of this bacterium, β-lytic protease (Blp). In the expression strains, blp gene expression under the regulation of the GroEL(A) and T5 promoters increased by 247- and 667-fold, respectively, as compared with the wild-type strain. After the cultivation of the expression strains L. capsici PGroEL(A)-blp and L. capsici PT5-blp, the Blp yield increased by 6.7- and 8.5-fold, respectively, with respect to the wild-type strain. The cultivation of the expression strain L. capsici PT5-blp was successfully scaled up. Under fermentation conditions the yield of the enzyme increased by 1.6-fold. The developed homologous system was used to express the gene of the bacteriolytic serine protease (Serp) of L. capsici VKM B-2533T. The expression of the serp gene in L. capsici PT5-serp increased by 585-fold. The developed homologous system for the gene expression of bacteriolytic Lysobacter enzymes is potentially biotechnologically valuable, and is promising for creating highly efficient expression strains.

show abstract

Stability of Therapeutic Enzymes: Challenges and Recent Advances

Ghosh

Alam

Rathore

et al. 2019

Advances in Experimental Medicine and Biology

View full text Add to dashboard Cite

Structural Studies of Component of Lysoamidase Bacteriolytic Complex from Lysobacter sp. XL1

Cited by 10 publications

References 25 publications

The potential of novel bacterial isolates from healthy ginseng for the control of ginseng root rot disease (Fusarium oxysporum)

The potential of novel bacterial isolates from healthy ginseng for the control of ginseng root rot disease (Fusarium oxysporum)

The First Homologous Expression System for the β-Lytic Protease of Lysobacter capsici VKM B-2533T, a Promising Antimicrobial Agent

Stability of Therapeutic Enzymes: Challenges and Recent Advances

Contact Info

Product

Resources

About