Structural properties of phospholipids in the rat liver inner mitochondrial membrane. A 31P-NMR study

Cullis, Pieter R.; Kruijff, B. de; Hope, Michael J.; Nayar, Rajiv; Rietveld, Anton; Verkleij, Arie J.

doi:10.1016/0005-2736(80)90466-6

Cited by 81 publications

(38 citation statements)

References 29 publications

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“…In most of these systems an isotropic 31p-NMR signal has been detected next to line shapes typical of lipids organized in the lamellar and H~I phases. Such mixtures are PE/egg PC [5], DOPE/DOPC/cholesterol [3], cardiolipin/egg PC in the presence of Ca 2+, MGlucDG/egg PC [3], total lipid extracts of E. coil [19], rod outer segments [30] and inner mitochondrial membranes [31]. Such an isotropic signal found next to bilayer and H~I phase spectra indicates isotropic motion of lipids in large structures.…”

Section: Hc H N Phase-lamellar Transition In Mixed Lipid Systemsmentioning

confidence: 96%

“…Cardiolipin adopts the H,, phase in the presence of cytochrome c [25,26]. Moreover, the H n phase can be induced by gramicidin [27,28], can be modulated in the presence of cholesterol [29], appears in complex lipid mixtures of synthetic lipids [9] and lipid extracts [19,30,31] from biological membranes.…”

Section: Iia Hexagonal II Phase Lipidsmentioning

confidence: 99%

“…The fact that almost every biological membrane contains a substantial amount of lipids that can adopt the nil phase upon isolation and the fact that total lipid extracts of several biomembranes do form non-bilayer structures under physiological conditions, strongly suggests a role for non-bilayer structures in biomembranes [19,30,31].…”

Section: Possible Non-bilayer Structures In Biologymentioning

confidence: 99%

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Lipidic intramembranous particles

Verkleij

1984

Biochimica et Biophysica Acta (BBA) - Reviews on Biomembranes

411

166

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Section: Hc H N Phase-lamellar Transition In Mixed Lipid Systemsmentioning

confidence: 96%

Section: Iia Hexagonal II Phase Lipidsmentioning

confidence: 99%

Section: Possible Non-bilayer Structures In Biologymentioning

confidence: 99%

See 1 more Smart Citation

Lipidic intramembranous particles

Verkleij

1984

Biochimica et Biophysica Acta (BBA) - Reviews on Biomembranes

411

166

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“…24; De Kruijff, B., unpublished data). In this respect it is interesting to note that also hydrated mitochondrial lipid extracts organize in non-bilayer structures upon addition of Ca 2 ÷ [7].…”

Section: Oe E I Pe _~mentioning

confidence: 99%

“…In mixtures with bilayer stabilizing lipids like phosphatidylcholine (PC), both PE and cardiolipin induce a variety of intermediate structures under appropriate conditions, such as lipidic particles extending into hexagonal tubes [5] and 0005-2736/82/0000-0000/$02.75 © 1982 Elsevier Biomedical Press hexagonal tubes dilating into stacked bilayers [6]. Such intermediate structures have also been found in the total lipid extract of mitochondrial membranes, especially upon addition of Ca 2÷ [7].…”

Section: Introductionmentioning

confidence: 99%

Possible role of non-bilayer lipids in the structure of mitochondria. A freeze-fracture electron microscopy study

Venetië

Verkleij

1982

Biochimica et Biophysica Acta (BBA) - Biomembranes

120

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Kinetics of glycolipid phase transitions: ms laser T‐jump synchrotron studies

Köberl

Hinz

Rappolt

et al. 1997

Ber Bunsenges Phys Chem

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The kinetics of the lamellar gel (Lp) to inverse hexagonal (Hll) phase transition as well as the L,+La phase change of aqueous suspensions of glycolipids were characterized using ms laser T-jump techniques in combination with synchrotron X-ray diffraction. The glycolipids employed were 1,2-di-O-alkyl-3-0-P-D-glucosyl-snglycerols with identical alkyl chains of 16 and 18 carbon atoms, respectively, and 1,2-di-O-alky1-3-O-P-D-galactosyl-sn-glycerol with C,, chains. The time course of the Lc-+La phase transition was probed using 1,2-di-O-hexadecyl-3-O-~-D-lactosyl-sn-glycerol, whose head group consists of the disaccharide P-D-galactopyranosyl-(1 -4)-P-D-glucopyranoside. Surprisingly the phase change from the lamellar to the inverse hexagonal topology is very fast despite the major structural changes involved in the lipid-water interface. The overall phase change of both glucolipids can be quantitatively described by a sequential mechanism of the type Lp A I A HIl.The intermediate I is thinner than both the Lp and the HI, phases. It has a relatively broad distribution of d-spacings and relaxes into the HI, phase with half times of approximately 125 ms (16-1,Z-Glc) and 244 ms (18-1,2-Glc), respectively. Both rate constants, k, and k,, are chain length dependent.The galactolipid transforms into the HI, phase following a sequential mechanism of the type Lg & I' % I & HII. The additional intermediate I' can be assigned to a lamellar phase bearing similarity to a thin La phase. The specific features of the galactose head group compared to glucose as head group that have been previously observed in equilibrium studies are also evident in the kinetic mechanism in that comparative structural changes in the intermediates are slower in the galactolipid than in the chain-homologous glucolipid.There is evidence from the X-ray data that the general transition mechanism is identical for gluco-and galactolipids. However, the first intermediate I' disappears too fast in the glucolipid phase change to be detectable with the present time resolution of the X-ray measurements.The kinetics of the phase change of the lactolipid is characterized within the resolution of the X-ray sequence (5ms) by a strict two-state mechanism L, --t La with transient coexistence of the initial and final phase during the kinetic phase transformation. The relaxation time has been found to be highly temperature dependent, being 57 ms at 79.1 "C and 14 ms at 81.1 "C.It is worth noting that there appears to be a principal difference in the phase change mechanism between glycolipids with mono-or disaccharide head groups in that the latter (16-1.2-Lac) shows no sign of any kinetic intermediate state. k k k k arrangements of the lipids, as seen in the HI, phase formed by a variety of lipids [8 -101. Prominent members of these lipids that form readily non-bilayer structures are glycolipids, both synthetic and natural, provided they have head groups that are small in surface area in comparison to the hydrophobic part of the molecule [l I]. Glycolipids show the most s...

show abstract

Structural properties of phospholipids in the rat liver inner mitochondrial membrane. A 31P-NMR study

Cited by 81 publications

References 29 publications

Lipidic intramembranous particles

Lipidic intramembranous particles

Possible role of non-bilayer lipids in the structure of mitochondria. A freeze-fracture electron microscopy study

Kinetics of glycolipid phase transitions: ms laser T‐jump synchrotron studies

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