Lipidic intramembranous particles

Verkleij, Arie J.

doi:10.1016/0304-4157(84)90003-0

Cited by 411 publications

(177 citation statements)

References 89 publications

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“…Since the samples were concentrated by centrifugation, the isotropic signal cannot be due to small structures (discs, micelles, small vesicles) but instead reflects high curvature within large structures. Small angle X-ray measurements at these ratios ( fig.3A) reveal an increased X-ray scattering around 60 ,h, when compared with a pure lipid dispersion (not shown) but no sharp reflections and thus no ordered stacking within the resolution limits, whereas electron microscopy in accordance with this visualizes highly curved bilayer structures fused to a threedimensional network with particles at the intersections ( fig.4A) morphologically similar to the so called 'honeycomb' or 'sponge' structures [25], and regular patterns of stacked lipidic particles.…”

Section: Resultsmentioning

confidence: 84%

Interaction of melittin with negatively charged phospholipids: Consequences for lipid organization

et al. 1987

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A characterization of the structural alterations induced by melittin in model-membranes of dioleoylphosphatidic acid and egg phosphatidylglycerol is presented, based on the use ofaxp-NMR, freeze-fracture electron microscopy and small angle X-ray scattering. In accordance with earlier findings on the cardiolipinmelittin system, melittin is found to have an inverted phase inducing effect on these negatively charged lipids, in contrast to the influence on zwitterionic phospholipids. In phosphatidic acid this is expressed in the formation of an HH phase; in phosphatidylglycerol a less ordered, non-lamellar structure with low water content is adopted.Melittin; Hexagonal HII phase; Protein-lipid interaction; 31p-NMR; Nonbilayer lipid structure; Freeze-fracture electron microscopy

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Section: Resultsmentioning

confidence: 84%

Interaction of melittin with negatively charged phospholipids: Consequences for lipid organization

et al. 1987

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“…Although it was most likely that the intramembranous particles in the freeze-fracture replica samples reflected the integral membrane proteins, the real nature of the TJ strands, lipids and/or proteins remained a mystery until the proteinaceous components constituting the TJ strands were identified (Kachar and Reese, 1982;Pinto da Silva and Kachar, 1982;Verkleij, 1984). Hence, when a novel integral membrane protein with four transmembrane domains of B60 kDa, termed occludin (Furuse et al, 1993), was identified as a component of TJ strands from hepatic junctional fractions (Tsukita and Tsukita, 1989), it was regarded as the Holy Grail in this field (Gumbiner, 1993).…”

Section: Molecular Bases For Tjmentioning

confidence: 99%

Tight junction-based epithelial microenvironment and cell proliferation

et al. 2008

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“…IMPs in replicas from living cells are generally believed to result from the presence of intrinsic membrane proteins (Branton, 1969), although IMPs were also demonstrated in arti¢cial lipid membranes in the total absence of proteins (Verkleij, 1984). In a recent paper (Eskandari et al, 1998), a relation was found between the size of IMPs resulting from expression of known proteins in the oocyte membrane and the number of K-helices spanning in the plasmalemma.…”

Section: Signi¢cance Of the Imp Changesmentioning

confidence: 99%

Quantal transmitter release by glioma cells: quantification of intramembrane particle changes

Bugnard¹,

Taulier²,

Bloc³

et al. 2002

Neuroscience

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AbstractöGlial cells in situ are able to release neurotransmitters such as glutamate or acetylcholine (ACh). Glioma C6BU-1 cells were used to determine whether the mechanisms of ACh release by a glial cell line are similar or not to quantal release from neurones. Individual C6BU-1 cells, pre-¢lled with ACh, were moved into contact with a Xenopus myocyte that was used as a real-time ACh detector. Upon electrical stimulation, C6BU-1 cells generated evoked ACh impulses which were Ca 2þ -dependent and quantal (quantal steps of ca. 100 pA). Changes in plasma membrane ultrastructure were investigated by using a freeze-fracture technique designed for obtaining large and £at replicas from monolayer cell cultures. A transient increase in the density of medium and large size intramembrane particles^and a corresponding decrease of small particles^occurred in the plasma membrane of C6BU-1 cells stimulated for ACh release. Changes in interaction forces between adjacent medium and large particles were investigated by computing the radial distribution function and the interaction potential. In resting cells, the radial distribution function revealed a signi¢cant increase in the probability to ¢nd two particles separated by an interval of 24 nm; the interaction potential suggested repulsive forces for intervals shorter than 24 nm and attractive forces between 24 and 26 nm. In stimulated cells, this interaction was displaced to 21 nm and made weaker, despite of the fact that the overall particle density increased. The nature of this transient change in intramembrane particles is discussed, particularly with regard to the mediatophore proteolipid which is abundant in the membranes C6-BU-1 like in those of cholinergic neurones. In conclusion, evoked ACh release from pre-¢lled C6-BU-1 glioma cells is quantal and Ca 2þ -dependent. It is accompanied by a transient changes in the size distribution and the organisation of intramembrane particles in the plasma membrane. Thus, for the release characteristics, glioma cells do not di¡er fundamentally from neurones. ß

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Lipidic intramembranous particles

Cited by 411 publications

References 89 publications

Interaction of melittin with negatively charged phospholipids: Consequences for lipid organization

Interaction of melittin with negatively charged phospholipids: Consequences for lipid organization

Tight junction-based epithelial microenvironment and cell proliferation

Quantal transmitter release by glioma cells: quantification of intramembrane particle changes

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