Structural parameters of collagen nerve grafts influence peripheral nerve regeneration

Stang, Felix; Fansa, Hisham; Wolf, Gerald; Reppin, Michael; Keilhoff, Gerburg

doi:10.1016/j.biomaterials.2004.07.060

Cited by 116 publications

(96 citation statements)

References 35 publications

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“…Stang et al reported that collagen does not seem to be a valuable material for an alternative nerve scaffold (26). The present study has shown, for the first time, that the rat CSF in collagen guide channel enhances peripheral nerve regeneration significantly more than the collagen guide channel without CSF in vivo.…”

Section: Sciatic Functional Index (Sfi)supporting

confidence: 44%

The effect of cerebro-spinal fluid in collagen guide channel on sciatic nerve regeneration in rat

Farjah

Dolatkhah²,

Pourheidar³

et al. 2015

Turkish Neurosurgery

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ABSTRACTgaps by inserting the severed nerve stumps into the two ends of the canal (16). Some natural and synthetic substances can be shaped in structures that are able to promote nerve regeneration for example: eggshell membrane (12), silicone (31), human amnion layer (22), collagen (18), collagen-Chitosan (36).Collagen-based biomaterials have been extensively studied as a promising nerve guide (7). Since collagen is a natural material, it shows excellent biocompatibility, insignificant immunogenicity, and high bio-absorbability (27).█ INTRODUCTION F or peripheral nerve repair, nerve autografts have always been considered as the "gold standard" for the restoration of structural and functional nerve regeneration (34).Autograft has several disadvantages, including the need for an extra-incision, loss of donor nerve function, mismatch in size between the donor nerve and the injured nerve, and a limited availability of donor nerve (33). To solve these problems, nerve guide channels (NGCs) have been made to bridge the nerve AIm: The aim of this study was to evaluate the effect of cerebrospinal fluid (CSF) in nerve regeneration across the collagen guide channel in comparison with autograft. mATERIAl and mEThODS: Forty adult male rats (250-300 g) were randomized into (1) collagen channel+CSF, (2) collagen channel+normal saline (NS), (3) autograft, and (4) sham surgery groups. The left sciatic nerve was exposed and a 10 mm nerve segment was cut and removed. In the collagen groups, the proximal and distal cuts ends of sciatic nerve were telescoped into the nerve guides and CSF or NS injected into collagen conduit. In the autograft group, the 10 mm nerve segment was turned backwards and used an autologous nerve graft. All animals were evaluated by sciatic functional index (SFI) and electrophysiology, histology, and immunohistochemistry testing. RESUlTS:The improvements in SFI since the beginning of the last evaluation in experimental groups were measured. On days 49 and 60 post-operation, the mean SFI of the collagen+CSF group was significantly greater than the autograft group (P< 0.05). On day 90, the mean nerve conduction velocity (NCV) of the collagen+CSF group was greater than autograft group (P< 0.05). The number of myelinated fibers in the collagen+CSF group was significantly greater than that of the collagen + NS group at day 90 (P<0.05).CONClUSION: CSF in collagen nerve guide channel effectively enhances nerve regeneration and promotes functional recovery in injured sciatic nerve of rats.

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Section: Sciatic Functional Index (Sfi)supporting

confidence: 44%

The effect of cerebro-spinal fluid in collagen guide channel on sciatic nerve regeneration in rat

Farjah

Dolatkhah²,

Pourheidar³

et al. 2015

Turkish Neurosurgery

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“…Also, collagen supports cell proliferation and tissue regeneration [65,73,104]. As nerve conduits collagen allows the establishment of topographical cues that guide axons to regrow [105,106] and has shown excellent cell adhesive properties that encourage cell attachment and proliferation [106,107]. The degradation time of the collagen conduits is relatively prolonged and takes up to 48 months which can cause nerve compression and fibrosis [84].…”

Section: Collagenmentioning

confidence: 99%

“…Uneven fibre distribution and nerve growth inhibition caused by fibre overlapping are some of the disadvantages associated with this type of conduits. However, association with wall guides helps to avoid this problem [105,187,188].…”

Section: Conduits Structure Modulationmentioning

confidence: 99%

Scaffolds for Peripheral Nerve Regeneration, the Importance of In Vitro and In Vivo Studies for the Development of Cell-Based Therapies and Biomaterials: State of the Art

Pedrosa¹,

Caseiro²,

Santos³

et al. 2017

Scaffolds in Tissue Engineering - Materials, Technologies and Clinical Applications

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Human adult peripheral nerve injuries are a high incidence clinical problem that greatly affects patients' quality of life. Although peripheral nervous system has intrinsic regenerative capacity, this occurs in an incomplete or poorly functional manner. When a nerve fiber loses its continuity with consequent damage of the basal lamina tubes, axon spontaneous regeneration is disorganized and mismatched. These phenomena translate in an inadequate nerve functional recovery and consequent musculoskeletal incapacity. Nerve grafts still remain the gold standard in peripheral injuries treatment. However, this approach contains its disadvantages such as the necessity of primary surgery to harvest the autografts, loss of a functional nerve, donor site morbidity and longer surgery procedures. Therefore, biomaterials and tissue engineering can provide efficient resources and alternatives to nerve injury repair not only by the development of biocompatible structures but also, introducing neurotrophic factors and cellular systems to stimulate optimum clinical outcome. In this chapter, a comprehensive state-of-the art picture of tissue-engineered nerve grafts scaffolds, their application in nerve regeneration along with latest advances in peripheral nerve repair and future perspectives will be discussed, including our own large experience in this field of knowledge.

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“…The user needs to maintain a sufficient balance between time of production (D) G-ratio was significantly lower in the autograft group versus all conduit groups. The ideal g-ratio is between 0.6 and 0.7 [30]. (E) There was no significant difference seen in the unmyelinated fibre diameter between all experimental groups (p < 0.05).…”

Section: Discussionmentioning

confidence: 92%

“…G-ratio was taken as the ratio of inner axonal diameter to the total axonal diameter and was used as a functional index of myelination. The ideal g-ratio was taken to be approximately between 0.6 and 0.7 [30]. All parameters were quantified using an upright florescent microscope (BX51 Upright Fluorescent Microscope Ò ; Olympus Inc.) and analysed using ImageJ image analysis software (NIH, Bethesda, USA).…”

Section: Morphology and Stereologymentioning

confidence: 99%

The effect of intraluminal contact mediated guidance signals on axonal mismatch during peripheral nerve repair

et al. 2012

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Publication InformationDaly, WT,Yao, L,Abu-rub, MT,O'Connell, C,Zeugolis, DI,Windebank, AJ,Pandit, AS (2012) b s t r a c tThe current microsurgical gold standard for repairing long gap nerve injuries is the autograft. Autograft provides a protective environment for repair and a natural internal architecture, which is essential for regeneration. Current clinically approved hollow nerve guidance conduits allow provision of this protective environment; however they fail to provide an essential internal architecture to the regenerating nerve. In the present study both structured and unstructured intraluminal collagen fibres are investigated to assess their ability to enhance conduit mediated nerve repair. This study presents a direct comparison of both structured and unstructured fibres in vivo. The addition of intraluminal guidance structures was shown to significantly decrease axonal dispersion within the conduit and reduced axonal mismatch of distal nerve targets (p < 0.05). The intraluminal fibres were shown to be successfully incorporated into the host regenerative process, acting as a platform for Schwann cell migration and axonal regeneration. Ultimately the fibres were able to provide a platform for nerve regeneration in a long term regeneration study (16 weeks) and facilitated increased guidance of regenerating axons towards their distal nerve targets.

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Structural parameters of collagen nerve grafts influence peripheral nerve regeneration

Cited by 116 publications

References 35 publications

The effect of cerebro-spinal fluid in collagen guide channel on sciatic nerve regeneration in rat

The effect of cerebro-spinal fluid in collagen guide channel on sciatic nerve regeneration in rat

Scaffolds for Peripheral Nerve Regeneration, the Importance of In Vitro and In Vivo Studies for the Development of Cell-Based Therapies and Biomaterials: State of the Art

The effect of intraluminal contact mediated guidance signals on axonal mismatch during peripheral nerve repair

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