Structural organization of polygalacturonase-encoding genes from Penicillium griseoroseum

Ribon, A. O. B.; Queiroz, Marisa Vieira de; Araújo, Elza Fernandes de

doi:10.1590/s1415-47572002000400020

Cited by 9 publications

(5 citation statements)

References 27 publications

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“…Nevertheless, was observed different results with P. griseoroseum, in which the parasexual cycle was not obtained spontaneously, and the diploids isolated by protoplast fusion were stable and produced few discrete haploid sectors even when placed in complete medium supplemented with benomil (38). High identity in amino acid sequence and similar global organization in PL and PG genes from P. expansum and P. griseoroseum was showed (5,12,(34)(35)(36). Penicillium expansum PLE1 shares 100% amino acids identity with PLG1 from P. griseoroseum and the organization of PL genes showed same hybridization pattern what suggests that PL genes have a similar global organization in the genome segment in which they are found.…”

Section: Introductionmentioning

confidence: 81%

“…Different methodologies which have been used to achieve these goals include protoplast production and regeneration, mutant isolation and characterization, protoplast transformation and isolation and characterization of genes coding pectinolytic enzyme in P. griseoroseum and P. expansum. (5,9,12,13,16,21,33,(34)(35)(36)38,41). Incompatibility was observed between these two species for the formation of heterokaryons and diploids when conidia were mixed (41).…”

Section: Introductionmentioning

confidence: 99%

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Morphological and molecular differentiation of the pectinase producing fungi Penicillium expansum and Penicillium griseoroseum

Cardoso¹,

Queiroz²,

Pereira³

et al. 2007

Braz. J. Microbiol.

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Two species from the genus Penicillium, Penicillium expansum and P. griseoroseum (Brasilian isolates) were characterized morphologic and molecularlly. Morphological variability was detected among isolates in regard to colony morphology and to conidia coloration. The molecular characterization was based on the RAPD markers, telomeric fingerprinting and ITS sequencing. A total of 78 RAPD primers were used and 8 presented differences in band patterns with 54% of the amplified polymorphic fragments. The monomorphic fragments of 600 bp (P. expansum) and 594 bp (P. griseoroseum) were amplified. The only internal transcribed spacer region variation detected between the two species was the additional six initial nucleotides. The analysis by telomeric fingerprinting showed polymorphism between both species and the chromosome minimal numbers estimated were three. The polymorphism observed in the organization of the subtelomeric region in the genome of two Penicillium species within the high homogeneous Penicillium subgenus is for the first time reported and perhaps can be employed in future phylogenetic studies.

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Section: Introductionmentioning

confidence: 81%

Section: Introductionmentioning

confidence: 99%

Morphological and molecular differentiation of the pectinase producing fungi Penicillium expansum and Penicillium griseoroseum

Cardoso¹,

Queiroz²,

Pereira³

et al. 2007

Braz. J. Microbiol.

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“…In this context, two polygalacturonase genes, isolated from P. olsonii , showing 56.3% similarity, did not hybridize with each other (Wagner et al , 2000). It is worth noting that most Penicillium species tested by Ribon et al (2002) showed the presence of at least two endopolygalacturonase genes, and gene families consisting of at least five pga members have been detected in Asperigillus niger (Parenicová et al , 1998, 2000) and Botrytis cinerea (Wubben et al , 1999).…”

Section: Resultsmentioning

confidence: 99%

Genomic organization of a polygalacturonase gene from a hyperpectinolytic mutant strain of Penicillium occitanis

Trigui-Lahiani

Ayadi

Hadj-Taieb

et al. 2008

FEMS Microbiology Letters

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The pga1 gene encoding an endopolygalacturonase was isolated from a hyperpectinolytic mutant strain of Penicillium occitanis. It consists of an ORF of 1.155 kbp encoding a putative protein of 346 amino acids with a predicted molecular mass of 39 kDa, belonging to the family 28 of glycosyl hydrolases. The deduced amino acid sequence comprises a putative 38 N terminal amino acids of the prepropeptide. The nature and position of amino acids comprising the active site as well as the overall three-dimensional structure were well conserved between the P. occitanis pga1 and all polygalacturonases. The coding region of the pga1 gene is interrupted by three short introns of 57, 53 and 65 bp in length. In addition to the determination of the transcription start site, the promoter sequence from the pga1 gene was analysed. It showed the conservation of known response elements for CreA and Hap2-3-4 factors. Southern blot analysis at high stringency shows that the isolated polygalacturonase gene exists as a single copy in the fungus genome. Northern blot analysis confirmed the constitutive hyperpectinolytic nature of the hyperpectinolytic CT1 mutation as high levels of pga1 mRNA were observed either on pectin or on glucose-grown cells.

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“…In this context, our group isolated and characterized two genes encoding PL ( plg 1 and plg 2) and two genes encoding PG ( pgg 2 and pgg 1) in P. griseoroseum (Ribon et al. 2002a,b; Bazzolli et al.…”

Section: Introductionmentioning

confidence: 99%

Improved pectinase production in Penicillium griseoroseum recombinant strains

Teixeira

Gonçalves

Queiroz

et al. 2011

Journal of Applied Microbiology

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Aims: To obtain recombinant strains of Penicillium griseoroseum that produce high levels of pectin lyase (PL) and polygalacturonase (PG) simultaneously. Methods and Results: A strain with high production of PL was transformed with the plasmid pAN52pgg2, containing the gene encoding PG of P. griseoroseum, under control of the gpd promoter gene from Aspergillus nidulans. Southern blot analysis demonstrated that all strain had at least one copy of pAN52pgg2 integrated into the genome. The recombinant strain P. griseoroseum T20 produced levels of PL and PG that were 266‐ and 27‐fold greater, respectively, than the wild‐type strain. Furthermore, the extracellular protein profile of recombinant T20 showed two protein bands of c. 36 and 38 kDa, associated with PL and PG, respectively. Conclusions: This recombinant strain T20 produces PL and PG using carbon sources of low costs, and an enzyme preparation that is free of cellulolytic and proteolytic activities. Significance and Impact of the Study: PL and PG play an important role in the degradation of pectin. Owing to their use in the juice and wines industries, there is a growing interest in the inexpensive production of these enzymes. This work describes an efficient system of protein expression and secretion using the fungus P. griseoroseum.

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Structural organization of polygalacturonase-encoding genes from Penicillium griseoroseum

Cited by 9 publications

References 27 publications

Morphological and molecular differentiation of the pectinase producing fungi Penicillium expansum and Penicillium griseoroseum

Morphological and molecular differentiation of the pectinase producing fungi Penicillium expansum and Penicillium griseoroseum

Genomic organization of a polygalacturonase gene from a hyperpectinolytic mutant strain of Penicillium occitanis

Improved pectinase production in Penicillium griseoroseum recombinant strains

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