Structural organization of plasma membrane lipids isolated from cells cultured as a monolayer and in tissue-like conditions

The Evolution of Three-Dimensional Cell Cultures Towards Unimpeded Regenerative Medicine and Tissue Engineering

“…[ [80][81][82]. Changes of the localization and properties of plasma membrane cholesterol and sphingomyelin in 3D are a probable cause for lowered oxidative stress, thus supporting the growing number of studies indicating that 2D cultures are an inappropriate, stressful method for cultivation of cells.…”

Cell proliferation in in vivo‐like three‐dimensional cell culture is regulated by sequestration of ERK1/2 to lipid rafts

“…Thus, we tend to concur with the notion of Liu et al ., suggesting that caveolin probably modulates signal transduction through an interaction with raft lipids rather than raft proteins . As the molecular order and lateral lipid organization of raft domains isolated from GD25β1 3D culture are different from rafts obtained from monolayer GD25β1 cells , it is quite possible that specific interactions, typical for a 3D in vivo ‐like environment, are responsible for retention of total as well as activated ERK1/2 in the detergent‐resistant membrane fraction of the 3D culture.…”

“…© 2014 John Wiley & Sons LtdCell Proliferation,47,[336][337][338][339][340][341][342][343][344][345][346] Proliferation in three-dimensional cell culture 339…”