Structural organization of bacterial cellulose: The origin of anisotropy and layered structures

Громовых, Т. И.; Пигалева, М. А.; Gallyamov, Marat O.; Ivanenko, I. P.; Ozerova, Kseniya E.; Kharitonova, Elena P.; Bahman, M. R.; Feldman, Nataliya B.; Луценко, С. В.; Kiselyova, Olga I.

doi:10.1016/j.carbpol.2020.116140

Cited by 39 publications

(30 citation statements)

References 40 publications

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“…The calculation admits that the BC moisture is 99.0%, which has been established many times by us through gravimetric analysis after freeze-drying. It is consistent well with the literature data [ 27 , 41 ].…”

Section: Methodssupporting

confidence: 94%

“…The BC yield subject to the shape and material of the vessel in which static culture is performed has been studied [ 26 ]. Assumptions have been made about the mechanisms and phases of BC formation [ 14 , 27 , 28 ].…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, we advanced a hypothesis that thin BC gel-films could repeatedly be taken out of the surface of the culture medium having an initial substrate loading that is optimum for BC biosynthesis (it is usually 20 g/L) [ 1 ]. It is well-known that the longer the biosynthesis, the more intergrown layers of gel-films are formed and the higher the overall thickness of the resultant gel-film [ 27 , 29 ]. However, if one sets an objective to produce thin gel-films, it is obvious that most of the substrate will remain unused.…”

Section: Introductionmentioning

confidence: 99%

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Biosynthesis of Bacterial Cellulose by Extended Cultivation with Multiple Removal of BC Pellicles

et al. 2021

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Extended cultivation with multiple removal of BC pellicles is proposed herein as a new biosynthetic process for bacterial cellulose (BC). This method enhances the BC surface area by 5–11 times per unit volume of the growth medium, improving the economic efficiency of biosynthesis. The resultant BC gel-films were thin, transparent, and congruent. The degree of polymerization (DP) and elastic modulus (EM) depended on the number of BC pellicle removals, vessel shape, and volume. The quality of BC from removals II–III to VII was better than from removal I. The process scale-up of 1:40 by volume increased DP by 1.5 times and EM by 5 times. A fact was established that the symbiotic Medusomyces gisevii Sa-12 was adaptable to exhausted growth medium: the medium was able to biosynthesize BC for 60 days, while glucose ran low at 24 days. On extended cultivation, DP and EM were found to decline by 39–64% and 57–65%, respectively. The BC gel-films obtained upon removals I–VI were successfully trialed in experimental tension-free hernioplasty.

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Section: Methodssupporting

confidence: 94%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Biosynthesis of Bacterial Cellulose by Extended Cultivation with Multiple Removal of BC Pellicles

et al. 2021

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“…Figure 4 illustrates SEM images of the BNC sheets. As can be seen in the images, all three sheets exhibit a representative architecture of cellulosic fibers: each BNC sheet represents a bundle of cellulosic nanofibrils as clusters of stretched cellulosic chains that form a spatial 3D structure which relates to individual and highly important characteristics of BNC [1,41]. Table 2 lists cellulose degrees of polymerization, strength behavior and TGA data for BNC.…”

Section: Biosynthesis Of Bncmentioning

confidence: 99%

Scale-Up of Biosynthesis Process of Bacterial Nanocellulose

et al. 2021

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Bacterial nanocellulose (BNC) is a unique product of microbiological synthesis, having a lot of applications among which the most important is biomedicine. Objective complexities in scaling up the biosynthesis of BNC are associated with the nature of microbial producers for which BNC is not the target metabolite, therefore biosynthesis lasts long, with the BNC yield being small. Thus, the BNC scale-up problem has not yet been overcome. Here we performed biosynthesis of three scaled sheets of BNC (each having a surface area of 29,400 cm2, a container volume of 441 L, and a nutrient medium volume of 260 L and characterized them. The static biosynthesis of BNC in a semisynthetic nutrient medium was scaled up using the Medusomyces gisevii Sa-12 symbiotic culture. The experiment was run in duplicate. The BNC pellicle was removed once from the nutrient medium in the first experiment and twice in the second experiment, in which case the inoculum and glucose were not additionally added to the medium. The resultant BNC sheets were characterized by scanning electron microscopy, capillary viscosimetry, infrared spectroscopy, thermomechanical and thermogravimetric analyses. When the nutrient medium was scaled up from 0.1 to 260 L, the elastic modulus of BNC samples increased tenfold and the degree of polymerization 2.5-fold. Besides, we demonstrated that scaled BNC sheets could be removed at least twice from one volume of the nutrient medium, with the yield and quality of BNC remaining the same. Consequently, the world’s largest BNC sheets 210 cm long and 140 cm wide, having a surface area of 29,400 cm2 each (weighing 16.24 to 17.04 kg), have been obtained in which an adult with burns or vast wounds can easily be wrapped. The resultant sheets exhibit a typical architecture of cellulosic fibers that form a spatial 3D structure which refers to individual and extremely important characteristics of BNC. Here we thus demonstrated the scale-up of biosynthesis of BNC with improved properties, and this result was achieved by using the symbiotic culture.

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“…In addition, BC can be used directly, without a dissolution step during the film production. The BC obtained process allows maintaining its 3D structure and crystallinity index above 85% (Azeredo et al, 2019;Gromovykh et al, 2020;Jozala et al, 2016;Keshk, 2014;Tsouko et al, 2015). The large amount of hydroxyl groups along the BC chains promote strong physical interactions resulting in a rigid structure in dried samples, driving to high Young's modulus and low elongation values (Cazon et al, 2019;Sun et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

UV-Shielding films of bacterial cellulose with glycerol and chitosan. Part 1: equilibrium moisture content and mechanical properties

Vázquez

Velázquez

Cazón

2021

CyTA - Journal of Food

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Bacterial cellulose films were combined with glycerol and chitosan to improve the UV-barrier and mechanical properties. In part 1, the moisture content and mechanical properties (tensile and puncture properties) were analysed by response surface methodology. The calculated values of the mechanical properties were in the range of 1.23-39.28 MPa for tensile strength, 2.28-25.34% for percentage of elongation, 11.72-1579.59 MPa for Young's modulus, 0.22-1.92 MJ/m 3 for toughness, 43.74-1067.35 g for burst strength and 0.39-3.12 mm for distance to burst. In general, a plasticizing and a reinforcing effect on the bacterial cellulose films was observed by glycerol and chitosan addition. Glycerol provided adhesive properties, which can be an advantage for application on foods, such as fruit or vegetables, as a wrapping material.Películas con protección UV de celulosa bacteriana, glicerol y quitosano. Parte 1: Contenido de humedad de equilibrio y propiedades mecánicas RESUMEN Se combinaron películas de celulosa bacteriana con glicerol y quitosano para mejorar las propiedades de barrera a la radiación UV y las propiedades mecánicas. En la parte 1, se analizaron el contenido de humedad y las propiedades mecánicas (propiedades de tracción y punción) mediante la metodología de superficie de respuesta. Los valores calculados de las propiedades mecánicas estuvieron en el rango de 1.23-39.28 MPa para resistencia a la tracción, 2.28-25.34% para porcentaje de alargamiento, 11.72-1579.59 MPa para módulo de Young, 0.22-1.92 MJ/m 3 para tenacidad, 43.74-1067.35 g para resistencia a la punción y 0.39-3.12 mm para la elasticidad de punción. En general, se observó un efecto plastificante y reforzante sobre las películas de celulosa bacteriana mediante la adición de glicerol y quitosano. El glicerol proporcionó propiedades adhesivas, que pueden ser una ventaja para su aplicación en alimentos, como frutas o verduras, como material de envoltura.

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Structural organization of bacterial cellulose: The origin of anisotropy and layered structures

Cited by 39 publications

References 40 publications

Biosynthesis of Bacterial Cellulose by Extended Cultivation with Multiple Removal of BC Pellicles

Biosynthesis of Bacterial Cellulose by Extended Cultivation with Multiple Removal of BC Pellicles

Scale-Up of Biosynthesis Process of Bacterial Nanocellulose

UV-Shielding films of bacterial cellulose with glycerol and chitosan. Part 1: equilibrium moisture content and mechanical properties

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