Structural mechanism underlying regulation of human EFhd2/Swiprosin-1 actin-bundling activity by Ser183 phosphorylation

Park, Kyoung Ryoung; An, Jin Yong; Kang, Jung Youn; Lee, Jung‐Gyu; Lee, Youngjin; Mun, Sang A; Jun, Chang‐Duk; Song, Woo Keun; Eom, Soo Hyun

doi:10.1016/j.bbrc.2016.12.124

Cited by 12 publications

(6 citation statements)

References 36 publications

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“…Swiprosin-1 has been reported to affect cell migration by remodeling the actin cytoskeleton (26)(27)(28). Our results showed that swiprosin-1 deletion restrained macrophage recruitment, which partly explained the attenuated proinflammatory cytokine production in the local tissues.…”

Section: Discussionsupporting

confidence: 62%

Swiprosin-1 deficiency impairs macrophage immune response of septic mice

Zhang

Sun

et al. 2018

JCI Insight

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Section: Discussionsupporting

confidence: 62%

Swiprosin-1 deficiency impairs macrophage immune response of septic mice

Zhang

Sun

et al. 2018

JCI Insight

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“…EF-hand domain-containing protein D2 ( EFHD2 ), upregulated after exposure to p , p‘- DDT, represents another protein with an altered expression that can bind actin [ 39 ]. EFHD2 participates in forming structures associated with actin, such as lamellipodia and membrane ruffles [ 40 ]. T-complex protein 1 subunit alpha ( TCPA ), downregulated after exposure to p , p‘- DDT, is a part of a chaperonin called TCC (chaperonin containing t-complex polypeptide 1) responsible for the proper folding of actin and tubulin [ 41 , 42 ].…”

Section: Discussionmentioning

confidence: 99%

Markers of acute toxicity of DDT exposure in pancreatic beta-cells determined by a proteomic approach

et al. 2020

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Many compounds have the potential to harm pancreatic beta-cells; organochlorine pollutants belong to those compounds. In this work, we aimed to find markers of acute toxicity of p,p‘-DDT exposure among proteins expressed in NES2Y human pancreatic beta-cells employing 2-D electrophoresis. We exposed NES2Y cells to a high concentration (150 μM, LC 96 after 72 hours) of p,p‘-DDT for 24 and 30 hours and determined proteins with changed expression using 2-D electrophoresis. We have found 22 proteins that changed their expression. They included proteins involved in ER stress (GRP78, and endoplasmin), mitochondrial proteins (GRP75, ECHM, IDH3A, NDUS1, and NDUS3), proteins involved in the maintenance of the cell morphology (EFHD2, TCPA, NDRG1, and ezrin), and some other proteins (HNRPF, HNRH1, K2C8, vimentin, PBDC1, EF2, PCNA, biliverdin reductase, G3BP1, FRIL, and HSP27). The proteins we have identified may serve as indicators of p,p‘-DDT toxicity in beta-cells in future studies, including long-term exposure to environmentally relevant concentrations.

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“…EF-hand domain-containing protein D2 ( EFHD2 ), upregulated after exposure to p,p‘- DDT, represents another protein with an altered expression that can bind actin (35). EFHD2 participates in the formation of structures associated with actin, such as lamellipodia and membrane ruffles (36). T-complex protein 1 subunit alpha ( TCPA ), downregulated after exposure to p,p‘- DDT, is a part of a chaperonin called TCC (chaperonin containing t-complex polypeptide 1) responsible for proper folding of actin and tubulin (37, 38).…”

Section: Discussionmentioning

confidence: 99%

Markers of Acute Toxicity in Pancreatic Beta-Cells Exposed to Lethal Doses of the Organochlorine Pollutant DDT Determined by a Proteomic Approach

Pavlíková

Šrámek

Jelínek

et al. 2020

Preprint

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Many compounds have the potential to harm pancreatic beta-cells; organochlorine pollutants belong to those compounds. In this work, we aimed to find markers of acute toxicity of p,p‘-DDT among proteins expressed in human pancreatic beta-cells NES2Y and visible at 2-D electrophoresis. We exposed NES2Y cells to a lethal dose of p,p‘-DDT (150 μM) for 24 hours and 30 hours and determined changes in protein expression using 2-D electrophoresis. We also stained the cells exposed to p,p‘-DDT to visualize the altered phenotype of the exposed cells. Among proteins with changed expression, we identified proteins involved in ER stress (GRP78, and endoplasmin), mitochondrial proteins (GRP75, ECHM, IDH3A, NDUS1, and NDUS3), proteins with potential to change the cell morphology (EFHD2, TCPA, NDRG1, and ezrin), and some other proteins (HNRPF, HNRH1, K2C8, vimentin, PBDC1, EF2, PCNA, biliverdin reductase, G3BP1, FRIL, and HSP27). These proteins can be used as markers of acute DDT toxicity.

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Structural mechanism underlying regulation of human EFhd2/Swiprosin-1 actin-bundling activity by Ser183 phosphorylation

Cited by 12 publications

References 36 publications

Swiprosin-1 deficiency impairs macrophage immune response of septic mice

Swiprosin-1 deficiency impairs macrophage immune response of septic mice

Markers of acute toxicity of DDT exposure in pancreatic beta-cells determined by a proteomic approach

Markers of Acute Toxicity in Pancreatic Beta-Cells Exposed to Lethal Doses of the Organochlorine Pollutant DDT Determined by a Proteomic Approach

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