Structural insights into the role of<i>Bacillus subtilis</i>YwfH (BacG) in tetrahydrotyrosine synthesis

Rajavel, Malligarjunan; Perinbam, Kumar; Gopal, B.

doi:10.1107/s0907444912046690

Cited by 10 publications

(8 citation statements)

References 31 publications

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“…The latter one seemed of special interest in a strain that had survived and overcome other competitors in a controlled environment. Among the 12B3-5 entries in this category, there were several peptides with antimicrobial activity like BacG protein [29], involved in the synthesis of the non-ribosomally synthesised dipeptide antibiotic produced by Bacillus subtillis; the E. faecalis macrolide-efflux protein A [30], able to regulate internal macrolide levels; or the PcfF protein, an accessory factor necessary to initiate the transfer of the tetracycline resistance plasmid pCF10 [31]. pCF10 is a member of a family of E. faecalis pheromone-inducible conjugative plasmids, which are determinants for ecological relations and strain communication in the gut environment, contributing to the GI tract competitive fitness [32].…”

Section: Discussionmentioning

confidence: 99%

Prevalence of an Intestinal ST40 Enterococcus faecalis over Other E. faecalis Strains in the Gut Environment of Mice Fed Different High Fat Diets

Sánchez

Cobo

Hidalgo

et al. 2020

IJMS

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E. faecalis is a commensal bacterium with specific strains involved in opportunistic and nosocomial infections. Therefore, it is important to know how the strains of this species are selected in the gut. In this study, fifteen E. faecalis strains, isolated over twelve weeks from the faeces of mice fed standard chow or one of three high fat diets enriched with extra virgin olive oil, refined olive oil or butter were subjected to a genetic “Multilocus Sequence Typing” study that revealed the presence of mainly two genotypes, ST9 and ST40, the latter one prevailing at the end of the research. A V3–V5 sequence comparison of the predominant ST40 strain (12B3-5) in a metagenomic study showed that this sequence was the only E. faecalis present in the mouse cohort after twelve weeks. The strain was subjected to a comparative proteomic study with a ST9 strain by 2D electrophoresis and mass spectrometry. After comparing the results with a E. faecalis database, unshared entries were compared and 12B3-5 showed higher antimicrobial production as well as greater protection from environmental factors such as xenobiotics, oxidative stress and metabolite accumulation, which could be the reason for its ability to outcompete other possible rivals in an intestinal niche.

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Section: Discussionmentioning

confidence: 99%

Prevalence of an Intestinal ST40 Enterococcus faecalis over Other E. faecalis Strains in the Gut Environment of Mice Fed Different High Fat Diets

Sánchez

Cobo

Hidalgo

et al. 2020

IJMS

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“…BacG (YwfH) is a NADPH-dependent reductase for stereoselective reduction of 3E-and 3Z-ex-H 2 HPP to tetrahydrohydroxyphenylpyruvate (H 4 HPP) 4R-and 4S-diastereomers which are then acted on by BacF and converted to corresponding H 4 Tyr diastereomers [35]. BacG structure was described at three conformational steps, providing a rationale for the ping-pong reaction mechanism [41]. BacF catalyzes reductive amination of the 2-keto group of the anticapsin candidate by using L-phenylalanine as an amino group donor [35].…”

Section: Biochemistry and Genetics Of Bacilysin Biosynthesismentioning

confidence: 99%

Biochemistry, genetics and regulation of bacilysin biosynthesis and its significance more than an antibiotic

Özcengiz

Öğülür

2015

New Biotechnology

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“…Subtilosin A and bacilysin participate in biocontrol efficacy of B. subtilis 9407 against BFB The synthetic genes srfAB, sboA, and bacG are essential for the synthesis of surfactin, subtilosin A, and bacilysin, respectively (Zheng et al 2000;Stein 2005;Rajavel et al 2013). Our previous studies showed that a lack of srfAB decreases surfactin production and colonization ability of B. subtilis 9407, thereby decreasing its biocontrol efficacy against BFB (Fan et al 2017b).…”

Section: Prediction Of Biosynthesis Gene Clusters In the Genome Sequence Of B Subtillis 9407mentioning

confidence: 99%

Comprehensive genomic analysis of Bacillus subtilis 9407 reveals its biocontrol potential against bacterial fruit blotch

Zeng

Wang

et al. 2021

Phytopathol Res

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Bacillus subtilis, a plant-beneficial bacterial species exhibiting good biocontrol capabilities, has been widely used in agricultural production. The endophytic strain 9407 can efficiently control bacterial fruit blotch (BFB) caused by the gram-negative bacterium Acidovorax citrulli. However, the mechanism underlying its biocontrol ability remains poorly understood. Given the genomic diversity of B. subtilis, strain 9407 was sequenced and assembled in this study to determine the genome information associated with its biocontrol traits. A combination of core genome phylogenetic analysis and average nucleotide identity (ANI) analysis demonstrated that the 9407 strain belonged to B. subtilis. Various functional genes related to biocontrol traits, i.e., biofilm formation, motility, pathogen inhibition, plant growth promotion, and induction of systemic resistance, were identified in B. subtilis 9407. Four secondary metabolite biosynthesis gene clusters with antibacterial ability were also found in the B. subtilis 9407 genome, including newly identified subtilosin A, bacilysin, and bacillaene, and the previously reported surfactin. Mutants lacking sboA or bacG, which are defective in synthesizing subtilosin A or bacilysin, showed decreased inhibitory activity against A. citrulli MH21, and the triple mutant with deleted sboA, bacG, and srfAB almost completely lost its inhibitory activity. The biofilm formation and swarming motility of the sboA and bacG mutants also decreased, in turn leading to decreased colonization on melon roots and leaves. Under greenhouse conditions, the biocontrol efficacy of the sboA and bacG mutants against BFB on melon leaves decreased by 21.4 and 32.3%, respectively. Here, we report a new biocontrol pathway of B. subtilis 9407 against BFB, in which subtilosin A and bacilysin contributed to the biocontrol efficacy by improving antibacterial activity and colonization ability of the strain. The comprehensive genomic analysis of B. subtilis 9407 improves our understanding of the biocontrol mechanisms of B. subtilis, providing support for further research of its biocontrol mechanisms and field applications.

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Structural insights into the role ofBacillus subtilisYwfH (BacG) in tetrahydrotyrosine synthesis

Cited by 10 publications

References 31 publications

Prevalence of an Intestinal ST40 Enterococcus faecalis over Other E. faecalis Strains in the Gut Environment of Mice Fed Different High Fat Diets

Prevalence of an Intestinal ST40 Enterococcus faecalis over Other E. faecalis Strains in the Gut Environment of Mice Fed Different High Fat Diets

Biochemistry, genetics and regulation of bacilysin biosynthesis and its significance more than an antibiotic

Comprehensive genomic analysis of Bacillus subtilis 9407 reveals its biocontrol potential against bacterial fruit blotch

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