Structural insights, biocatalytic characteristics, and application prospects of lignin-modifying enzymes for sustainable biotechnology

Singh, Anil Kumar; Iqbal, Hafiz M.N.; Cardullo, Nunzio; Muccilli, Vera; Fernández‐Lucas, Jesús; Schmidt, Jens Ejbye; Jesionowski, Teofil; Bilal, Muhammad

doi:10.1016/j.ijbiomac.2023.124968

Cited by 6 publications

(4 citation statements)

References 354 publications

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“…White-rot fungi secrete a number of oxidoreductases that are involved in lignin depolymerization [ 23 , 24 ]. These oxidoreductases encompass heme-containing peroxidases (manganese, lignin, and versatile peroxidases) and copper-dependent polyphenol oxidases named laccases (E.C.…”

Section: Introductionmentioning

confidence: 99%

Efficient Decolorization of the Poly-Azo Dye Sirius Grey by Coriolopsis gallica Laccase-Mediator System: Process Optimization and Toxicity Assessment

Zouari-Mechichi,

Benali,

Alessa

et al. 2024

Molecules

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The textile industry produces high volumes of colored effluents that require multiple treatments to remove non-adsorbed dyes, which could be recalcitrant due to their complex chemical structure. Most of the studies have dealt with the biodegradation of mono or diazo dyes but rarely with poly-azo dyes. Therefore, the aim of this paper was to study the biodegradation of a four azo-bond dye (Sirius grey) and to optimize its decolorization conditions. Laccase-containing cell-free supernatant from the culture of a newly isolated fungal strain, Coriolopsis gallica strain BS9 was used in the presence of 1-hydroxybenzotriazol (HBT) to optimize the dye decolorization conditions. A Box–Benken design with four factors, namely pH, enzyme concentration, HBT concentration, and dye concentration, was performed to determine optimal conditions for the decolorization of Sirius grey. The optimal conditions were pH 5, 1 U/mL of laccase, 1 mM of HBT, and 50 mg/L of initial dye concentration, ensuring a decolorization yield and rate of 87.56% and 2.95%/min, respectively. The decolorized dye solution showed a decrease in its phytotoxicity (Germination index GI = 80%) compared to the non-treated solution (GI = 29%). This study suggests that the laccase-mediator system could be a promising alternative for dye removal from textile wastewater.

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Section: Introductionmentioning

confidence: 99%

Efficient Decolorization of the Poly-Azo Dye Sirius Grey by Coriolopsis gallica Laccase-Mediator System: Process Optimization and Toxicity Assessment

Zouari-Mechichi,

Benali,

Alessa

et al. 2024

Molecules

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“…Laccases (Lacs) are multicopper enzymes that catalyze the oxidation of phenol to phenoxy radicals with the concomitant reduction of O 2 to water [ 16 ]. Unlike many other enzymes, laccases have a wide range of substrates, including various types of phenols and polyphenols (including lignin), polyamines, aryl diamines and some inorganic ions.…”

Section: Introductionmentioning

confidence: 99%

“…Unlike many other enzymes, laccases have a wide range of substrates, including various types of phenols and polyphenols (including lignin), polyamines, aryl diamines and some inorganic ions. In addition, it has been shown that low-molecular-weight mediators, such as 1-hydroxy-benzo-triazole (HBT), can boost lignin oxidation at a distance from the enzyme active site and react with parts of the lignin polymer not accessible to Lacs enzyme alone [ 16 ]. Due to the complex structure of lignin, reaction mechanisms at the molecular level have mainly been studied using small model compounds representing structurally diverse lignins, and the reactions of different lignin model compounds in the presence and absence of mediators have been reviewed [ 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

Modification of a Marine Pine Kraft Lignin Sample by Enzymatic Treatment with a Pycnoporus cinnabarinus Laccase

Malric-Garajova,

Fortuna,

Pion

et al. 2023

Molecules

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Here, we report work on developing an enzymatic process to improve the functionalities of industrial lignin. A kraft lignin sample prepared from marine pine was treated with the high-redox-potential laccase from the basidiomycete fungus Pycnoporus cinnabarinus at three different concentrations and pH conditions, and with and without the chemical mediator 1-hydroxybenzotriazole (HBT). Laccase activity was tested in the presence and absence of kraft lignin. The optimum pH of PciLac was initially 4.0 in the presence and absence of lignin, but at incubation times over 6 h, higher activities were found at pH 4.5 in the presence of lignin. Structural changes in lignin were investigated by Fourier-transform infrared spectroscopy (FTIR) with differential scanning calorimetry (DSC), and solvent-extractable fractions were analyzed using high-performance size-exclusion chromatography (HPSEC) and gas chromatography–mass spectrometry (GC–MS). The FTIR spectral data were analyzed with two successive multivariate series using principal component analysis (PCA) and ANOVA statistical analysis to identify the best conditions for the largest range of chemical modifications. DSC combined with modulated DSC (MDSC) revealed that the greatest effect on glass transition temperature (Tg) was obtained at 130 U g cm−1 and pH 4.5, with the laccase alone or combined with HBT. HPSEC data suggested that the laccase treatments led to concomitant phenomena of oligomerization and depolymerization, and GC–MS revealed that the reactivity of the extractable phenolic monomers depended on the conditions tested. This study demonstrates that P. cinnabarinus laccase can be used to modify marine pine kraft lignin, and that the set of analytical methods implemented here provides a valuable tool for screening enzymatic treatment conditions.

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“…Innovative immobilization techniques have been developed to create chemically bonded immobilized enzymes superior to free enzymes in terms of stability and activity (Othman et al, 2021 , 2023 ). The ligninolytic enzymes are being researched as prospective environmental enzymes to exchange the conventional mechanical and chemical courses in several industries, comprising nanoparticle-based bio-devices, pharmaceuticals, pulp and paper, and textile industries (Singh et al, 2023 ). In light of this background, this particular Research Topic has been created to concentrate on innovative, cutting-edge, and promising advancements in the study of microbial ligninolytic enzymes.…”

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confidence: 99%

Editorial: Ligninolytic enzymes and their potential applications

et al. 2023

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Structural insights, biocatalytic characteristics, and application prospects of lignin-modifying enzymes for sustainable biotechnology

Cited by 6 publications

References 354 publications

Efficient Decolorization of the Poly-Azo Dye Sirius Grey by Coriolopsis gallica Laccase-Mediator System: Process Optimization and Toxicity Assessment

Efficient Decolorization of the Poly-Azo Dye Sirius Grey by Coriolopsis gallica Laccase-Mediator System: Process Optimization and Toxicity Assessment

Modification of a Marine Pine Kraft Lignin Sample by Enzymatic Treatment with a Pycnoporus cinnabarinus Laccase

Editorial: Ligninolytic enzymes and their potential applications

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