Structural identification of a fucose-containing 1,3-β-mannoglucan from Poria cocos and its anti-lung cancer CL1-5 cells migration via inhibition of TGFβR-mediated signaling

Lin, Tsai-Yun; Lü, Mei; Chang, Chia Chuan

doi:10.1016/j.ijbiomac.2020.04.014

Cited by 24 publications

(14 citation statements)

References 29 publications

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“…Tang proved that PAC could inhibit the phosphorylation of the ERK signaling pathway by downregulating the expression of p-ERK1/2, which indicated that PAC could inhibit the proliferation and promote apoptosis of HeLa cells [52]. Lin et al demonstrated that FMGP inhibited the migration of lung cancer CL1-5 cells by downregulating TGFβRI expression and simultaneously decreasing the phosphorylation levels of FAK and Akt [53]. CMP3 induced HepG2 cell apoptosis through two pathways.…”

Section: E Antitumor Effect Of Pacmentioning

confidence: 99%

Phytochemistry and Biological Activities of Poria

Lü

Tian

Zhou

et al. 2021

Journal of Chemistry

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Poria is a common Traditional Chinese Medicine in clinic. In recent years, the chemical and pharmacological studies of Poria have made great progress, triterpenes and polysaccharides have been isolated, and various types of compounds containing lipids, octanoic acids, fatty acids, and trace elements have been found. In this paper, we reviewed the literature, summarized the main compound types, and reviewed in detail their pharmacological effects in antitumor, immunomodulatory, effects on kidney, hepatoprotective activity, effects on blood sugar, antioxidant effects, anti-inflammatory effects, effects on the gut, antidepressant, and so on, and also categorized the compounds with the same or similar pharmacological effects to provide a reference for the in-depth study of the material basis of the pharmacological effect, quality standards, and pharmacological activity of Poria.

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Section: E Antitumor Effect Of Pacmentioning

confidence: 99%

Phytochemistry and Biological Activities of Poria

Lü

Tian

Zhou

et al. 2021

Journal of Chemistry

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“…( Jiang et al, 2019 ; Xu et al, 2019 ). Scientific research proved that single TCM extracts, such as Astragali Radix , and Poria , have certain effects on inducing lung cancer cell apoptosis, inhibiting cell invasion, metastasis, and angiogenesis ( Cheng et al, 2014 ; Lee et al, 2018 ; Xu et al, 2018 ; Lin et al, 2020 ). Modified Sijunzi Decoction (MSJZD) was composed of Astragali Radix , Poria , Codonopsis Radix , Rhizoma Atractylodis Macrocephalae , Pinelliae Rhizoma , Fritillariae Thunbergii Bulbus , Herba Hedyotis , and Glycyrrhizae Praeparata cum Melle Radix et Rhizoma .…”

Section: Introductionmentioning

confidence: 99%

Modified Sijunzi Decoction Inhibits Epithelial-Mesenchymal Transition of Non-Small Cell Lung Cancer by Attenuating AKT/GSK3β Pathway in vitro and in vivo

et al. 2022

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Modified Sijunzi Decoction (MSJZD) is an empirical prescription of Traditional Chinese Medicine (TCM) and has been corroborated to be effective in multiple human diseases, but its role in non-small cell lung cancer (NSCLC) is enigmatic. Here we mainly analyze the function and mechanism of MSJZD in NSCLC. In this study, we used a method that coupled ultra-performance liquid chromatography to quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) to investigate the major constituents in MSJZD with positive and negative ion modes. Additionally, in in vitro experiments, the effects of serum-containing MSJZD on the biological behavior of NSCLC cells induced by TGF-β1 were assessed by cell function experiments. Then, the influences of serum-containing MSJZD on epithelial-mesenchymal transition (EMT)-related markers were examined by immunofluorescence and western blot assays. Also, the AKT/GSK3β pathway and apoptosis-related markers were estimated by western blotting. Tumor xenografts were generated by subcutaneously injecting A549 cells into BALB/c nude mice to determine the effects of MSJZD in vivo. We first analyzed the composition of MSJZD. In positive ion mode, 47 kinds of components were identified. In negative ion mode, 45 kinds of components were identified. We also found that TGF-β1 contributed to inducing cell morphological changes and EMT progression. In vitro, surprisingly, cell proliferation, migration as well as invasion in NSCLC cells induced by TGF-β1, could be weakened by serum-containing MSJZD, and apoptosis was intensified. Moreover, serum-containing MSJZD weakened EMT passage and AKT/GSK3β pathway activation and induced apoptosis-related markers in NSCLC cells triggered by TGF-β1. In vivo, we discovered that MSJZD attenuated the tumor growth, promoted histopathological damage, and induced apoptosis in A549 tumor-bearing mice. Importantly, MSJZD has also restrained the development of EMT, AKT/GSK3β pathway, and TGF-β1 expression levels in nude mice. These findings demonstrated that MSJZD significantly weakened NSCLC progression by modulating EMT and AKT/GSK3β pathway.

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“…Studies showed that W. cocos provided with anti-inflammatory, antitumor, immune regulation, antioxidation, diuretic effects, and so on. [1][2][3][4][5] Therefore, W. cocos has attracted wide attention due to its high medicinal value, nutritional value, and unique health care function. However, its quality problems are easily affected by the environment and are regional dependent that different regions will have different factors to consider.…”

Section: Introductionmentioning

confidence: 99%

Data fusion of multiple‐information strategy based on Fourier transform near infrared spectroscopy and Fourier‐transform mid infrared for geographical traceability of Wolfiporia cocos combined with chemometrics

Zhang²,

Zuo

et al. 2022

Journal of Chemometrics

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Owing to the widespread concern relating to herb safety and quality, there is a momentum to discriminate the geographical traceability of fungus with multiple‐information technologies. In this study, we attempted to evaluate the fusion strategy of multiple‐information for the geographical traceability of this fungus based on Fourier transform near infrared spectroscopy (FT‐NIR) and Fourier‐transform mid infrared spectroscopy (FT‐MIR) with chemometrics. From all results, (1) comparative visualization of t‐distributed stochastic neighbor embedding (t‐SNE) emerged that the superior effect of FT‐NIR spectroscopy was better than FT‐MIR, which were separated according to the samples of different regions; (2) in view of optimized spectrum, the geographical traceability of extreme learning machine (ELM) model was better than the partial least squares discriminant analysis (PLS‐DA) and support vector machine (SVM) models that the accuracy of training set for all models was 100%; (3) the accuracy of train and test for ELM model were 100% with single spectrum, low‐level, and mid‐level fusion of FT‐NIR and FT‐MIR based on principal components (PCs) and competitive adaptive weighting algorithm (CARS), which was suitable for geographical traceability and evaluation of this fungus. (4) The three models based on successive projection algorithm (SPA) were not dissatisfied for the geographical traceability of this fungus. (5) The result of hierarchical cluster analysis (HCA) was consistent with the above results, which could distinguish samples according to different regions. All results showed that models could be developed as superior model on the foundation of single spectrum based on FT‐NIR, low‐level or mid‐level (except SPA) fusion of ELM, which could be employed in geographical traceability of Wolfiporia cocos, effectively.

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Structural identification of a fucose-containing 1,3-β-mannoglucan from Poria cocos and its anti-lung cancer CL1-5 cells migration via inhibition of TGFβR-mediated signaling

Cited by 24 publications

References 29 publications

Phytochemistry and Biological Activities of Poria

Phytochemistry and Biological Activities of Poria

Modified Sijunzi Decoction Inhibits Epithelial-Mesenchymal Transition of Non-Small Cell Lung Cancer by Attenuating AKT/GSK3β Pathway in vitro and in vivo

Data fusion of multiple‐information strategy based on Fourier transform near infrared spectroscopy and Fourier‐transform mid infrared for geographical traceability of Wolfiporia cocos combined with chemometrics

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