Structural features and assessment of prebiotic activity of refined arabinoxylooligosaccharides from wheat bran

Enzymatic production of wheat and ryegrass derived xylooligosaccharides and evaluation of their in vitro effect on pig gut microbiota

“…perfringens, 8.8 ± 0.1 log cfu/g lactic acid bacteria 2 Negative control was a tube containing buffer instead of oligosaccharide Table 3 Effect of arabinoxylooligosaccharides (AXOS) and xylooligosaccharides (XOS) prepared from hydrothermally pretreated ryegrass pulp using endo-1,4-β-xylanases from different sources on in vitro growth of various microbial groups from pig faeces 1 The feces slurry contained 5.4 ± 0.2 log cfu/g Enterobacteriaceae, 2.6 ± 0.5 log cfu/g Cl. perfringens, 8.6 ± 0.5 log cfu/g lactic acid bacteria 2 Negative control contained buffer instead of oligosaccharide 3 Positive control contained fructooligosaccharides (FOS) from chicory instead of the experimental oligosaccharides 4 Hydrothermal pretreatment supernatant was obtained after autoclaving milled RG at 140°C for 60 min derived from many natural polysaccharides have been shown to have prebiotic effects (e.g., FOS, galactooligosaccharides, maltooligosaccharides, and gentiooligosaccharides) [25]; and FOS are now accepted as the golden standard of prebiotics [26]. In order to evaluate the oligosaccharide effect on pig gut health, oligosaccharide samples prepared in this study were incubated with pig fecal microbiota under anaerobic conditions.…”

Evaluation of the prebiotic potential of arabinoxylans from brewer’s spent grain

“…They are composed of β-D-(1 →4)-linkedxylopyranosyl residues to which α-L-arabinofuranose units are linked as side chains and some of them are substituted with monomeric or dimeric ferulic acid residues (Grootaert et al 2007). Studies reported on the AX fermentation by the human intestinal bacteria indicated that fermentation of AX promotes the growth of Bifidobacterium, Lactobacillus and Eubacterium groups (Cloetens et al 2010;Grootaert et al 2007;Gullón et al 2014;Hughes et al 2007;Pastell et al 2009;Vardakou et al 2008) and, in a smaller extent, also promotes the growth of Bacteroides and Clostridium species (Hughes et al 2007). This process results in the formation of bacterial metabolites such as SCFA and specially the increase of propionic acid concentration (Kabel et al 2002;Napolitano et al 2009;Rose et al 2009).…”

“…The main cereal source of AX is wheat or wheat bran (Gullón et al 2014;Hughes et al 2007;Pastell et al 2009;Rose et al 2009) due to its high content of AX. Also, and to a less extent, the fermentation of AX was reported in rye (Pastell et al 2009), rice and maize brans (Rose et al 2009) and brewer's spent grain (BSG) (Kabel et al 2002).…”

Pre-treatment and extraction techniques for recovery of added value compounds from wastes throughout the agri-food chain

“…60 Hemicelluloses can also be hydrolysed to their constituent sugars and converted into fuel, ethanol and other value added chemicals, such as furfural, levulinic acid, xylitol and 5-hydroxymethylfurfural (HMF). 33 67 who investigated the impact of the pre-treatment severity using steam explosion on the release and decomposition of xylan hemicelluloses from wheat straw in relation to the enzymatic digestibility of the remaining cellulose.…”

“…76 There is an increasing body of work describing the successful hydrolysis and extraction of hemicellulose-derived prebiotics (oligo and polysaccharides). For example, Gullon et al 77 subjected wheat bran to a two-stage process (aqueous extraction followed by hydrothermal treatment) to produce xylan-derived oligosaccharides. These were evaluated for prebiotic activity and demonstrated the ability to enhance bifidobacterial proliferation to the same level as fructo-oligosaccharides.…”