Structural features and anticoagulant activities of a novel natural low molecular weight heparin from the shrimp Penaeus brasiliensis

Dietrich, Carl P.; Paiva, J F; Castro, Ricardo A.B.; Chavante, Suely F.; Jeske, Walter; Fareed, Jawed; Gorin, Philip A.J.; Mendes, Aline; Nader, Helena B.

doi:10.1016/s0304-4165(99)00087-2

Cited by 46 publications

(45 citation statements)

References 15 publications

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“…TOCSY spectra were run with 4096 ϫ 400 points with a spin-lock field of ϳ10 kHz and a mixing time of 80 ms. HMQC spectra were run with 1024 ϫ 256 points and globally optimized alternating phase rectangular pulses for decoupling. NOESY spectra were run with a mixing time of 100 ms. Chemical shifts are relative to external trimethysilylpropionic acid at 0 ppm for 1 H and to methanol for 13 C. Anticoagulant Activity of the Galactofucan-All the coagulation assays (prothrombin time, activated partial thromboplastin time; TT, thrombin time, and HEPTEST®) were performed with a coagulometer, as described earlier (33), and were measured by using human plasma from Roche Applied Science. All assays were performed in duplicate and repeated at least three times on different days (n ϭ 6).…”

Section: Methodsmentioning

confidence: 99%

Structural and Hemostatic Activities of a Sulfated Galactofucan from the Brown Alga Spatoglossum schroederi

Rocha

Moraes

Trindade

et al. 2005

Journal of Biological Chemistry

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142

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The brown alga Spatoglossum schroederi contains three fractions of sulfated polysaccharides. One of them was purified by acetone fractionation, ion exchange, and molecular sieving chromatography. It has a molecular size of 21.5 kDa and contains fucose, xylose, galactose, and sulfate in a molar ratio of 1.0:0.5:2.0:2.0 and contains trace amounts of glucuronic acid. Chemical analyses, methylation studies, and NMR spectroscopy showed that the polysaccharide has a unique structure, composed of a central core formed mainly by 4-linked ␤-galactose units, partially sulfated at the 3-O position. Approximately 25% of these units contain branches of oligosaccharides (mostly tetrasaccharides) composed of 3-sulfated, 4-linked ␣-fucose and one or two nonsulfated, 4-linked ␤-xylose units at the reducing and nonreducing end, respectively. This sulfated galactofucan showed no anticoagulant activity on several "in vitro" assays. Nevertheless, it had a potent antithrombotic activity on an animal model of experimental venous thrombosis. This effect is time-dependent, reaching the maximum 8 h after its administration compared with the more transient action of heparin. The effect was not observed with the desulfated molecule. Furthermore, the sulfated galactofucan was 2-fold more potent than heparin in stimulating the synthesis of an antithrombotic heparan sulfate by endothelial cells. Again, this action was also abolished by desulfation of the polysaccharide. Because this sulfated galactofucan has no anticoagulant activity but strongly stimulates the synthesis of heparan sulfate by endothelial cells, we suggested that this last effect may be related to the "in vivo" antithrombotic activity of this polysaccharide. In this case the highly sulfated heparan sulfate produced by the endothelial cells is in fact the antithrombotic agent. Our results suggested that this sulfated galactofucan may have a potential application as an antithrombotic drug.

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Section: Methodsmentioning

confidence: 99%

Structural and Hemostatic Activities of a Sulfated Galactofucan from the Brown Alga Spatoglossum schroederi

Rocha

Moraes

Trindade

et al. 2005

Journal of Biological Chemistry

Self Cite

142

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“…Interestingly, a Hep-like HS was reported to exist in shrimp heads, an industrial waste [11]. Therefore, in this study, the structure and biological activities of a HS preparation extracted from shrimp heads were analyzed.…”

Section: Substantial Quantitative Differences Between Hs and Hep Exismentioning

confidence: 99%

“…Therefore, in this study, the structure and biological activities of a HS preparation extracted from shrimp heads were analyzed. [11].…”

Section: Substantial Quantitative Differences Between Hs and Hep Exismentioning

confidence: 99%

Unique heparan sulfate from shrimp heads exhibits a strong inhibitory effect on infections by dengue virus and Japanese encephalitis virus

Chen

Yamada

Hama

et al. 2011

Biochemical and Biophysical Research Communications

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The structure and biological activities of a highly sulfated heparan sulfate (HS) extracted from shrimp (Penaeus brasiliensis) heads were characterized. Structurally the shrimp HS was more heterogenous than heparin, although it is still highly sulfated. The molecular mass of the shrimp HS preparation was determined to be 32.3 kDa by gel filtration HPLC. Analysis by surface plasmon resonance demonstrated that various growth/differentiation factors specifically bound to the shrimp HS with comparable affinity. Notably, the shrimp HS had a greater inhibitory effect against infections by dengue virus type 2 as well as Japanese encephalitis virus than heparin. Experiments on anticoagulant activity indicated that the shrimp HS exhibited significant anti-thrombin activity, but less than the commercial heparin. Hence, the HS preparation from shrimp heads, an industrial waste, is a prospective agent for a variety of clinical applications.

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“…Vários grupos de pesquisa vêm se dedicando ao estudo de polissacarídeos extraídos de invertebrados marinhos, [137][138][139][140][141][142][143] 150 O Agaricus blazei, que é um cogumelo comestível -conhecido popularmente como cogumelo do sol, representa um caso emblemático. Ele é oriundo das regiões serranas do sul do estado de São Paulo.…”

Section: Outros Polissacarídeosunclassified

Polissacarídeos da biodiversidade brasileira: uma oportunidade de transformar conhecimento em valor econômico

2009

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Recebido em 21/1/09; aceito em 3/3/09; publicado na web em 17/3/09 POLYSACCHARIDES FROM BRAZILIAN BIODIVERSITY: AN OPPORTUNITY TO CHANGE KNOWLEDGE INTO ECONOMIC VALUE. A brief comment about general characteristics of polysaccharide was presented. Brazilian trade of polysaccharides was obtained from the "Ministério de Desenvolvimento, Indústria e Comércio Exterior" -Brazil. A list of these products was prepared and their price and amount analyzed in the period of 1998-2007. Some chemical properties and application of polysaccharides from our biodiversity was described. In this review they were classified by origin, in vegetal (exudate, seed, fruit, seaweed), animal and bacteria source. There is a trade deficit that can be reverted if part of the accumulated scientific knowledge was used to promote the national economic development in the field.Keywords: polysaccharide; Brazilian biodiversity; economic development. introduçãoBiodiversidade foi definida por Arnt, de uma forma bem criativa, como sendo: 1 "o cofre de um patrimônio químico inexplorado de remédios, alimentos, fertilizantes, pesticidas, cosméticos, solventes, fermentos, têxteis, plásticos, celulose, óleos e energia, além de moléculas, enzimas e genes em número quase infinito."A diversidade contida no cofre é inexata. Estima-se que a diversidade da fauna nacional seja de cerca de 2 milhões de espécies de animais, exceto insetos, representando 14% da biota mundial. Para conhecê-la estima-se que seriam necessários mais dez séculos de trabalho, se o ritmo atual de estudo fosse mantido. 2 Com relação à flora, acredita-se que existam no país cerca de 55 mil espécies vegetais (22% do total mundial), 3 muitas ainda desconhecidas.O valor econômico da nossa diversidade biológica é de difícil mensuração. Uma estimativa é de que seja próximo de 4 trilhões de dólares. 4 Apesar desse elevado "valor econômico", sabe-se que ela por si só não é garantia de crescimento econômico. Transformar biodiversidade em desenvolvimento, através do seu uso sustentado e de seus recursos derivados, é o grande desafio. A etapa inicial para vencer esse desafio é transformar conhecimento em valor econômico e em bem-estar da população, uma das maiores preocupações das sociedades atuais.Diversos produtos utilizados pela sociedade têm como fonte a flora brasileira. Medicamentos, alimentos e seus aditivos, fibras, óleos naturais e essenciais, cosméticos, produtos químicos, biocombustível, são alguns bons exemplos. Inúmeras são as classes de compostos químicos que podem ser extraídos das nossas espécies vegetais. Uma delas é representada pelos polissacarídeos. Pretende-se discorrer sobre o estado atual do conhecimento científico dos polissacarídeos da biodiversidade brasileira, e pontuar o potencial e a necessidade de aplicação industrial. características Gerais de PolissacarídeosPolissacarídeos são polímeros naturais, os quais podem ser constituídos de um único ou de diferentes tipos de monossacarídeos. Celulose, alginato e goma arábica são exemplos de homo-, co-, e hetero-polissacarídeos, r...

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Structural features and anticoagulant activities of a novel natural low molecular weight heparin from the shrimp Penaeus brasiliensis

Cited by 46 publications

References 15 publications

Structural and Hemostatic Activities of a Sulfated Galactofucan from the Brown Alga Spatoglossum schroederi

Structural and Hemostatic Activities of a Sulfated Galactofucan from the Brown Alga Spatoglossum schroederi

Unique heparan sulfate from shrimp heads exhibits a strong inhibitory effect on infections by dengue virus and Japanese encephalitis virus

Polissacarídeos da biodiversidade brasileira: uma oportunidade de transformar conhecimento em valor econômico

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