Structural, evolutionary and functional analysis of APN genes in the Lepidoptera Bombyx mori

Lin, Ping; Cheng, Tingcai; Jin, Shengkai; Jiang, Liang; Wang, Chen; Xia, Qingyou

doi:10.1016/j.gene.2013.11.002

Cited by 20 publications

(31 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several studies have revealed that multiple gene families developed from APN genes before the evolutionary divergence of Lepidoptera . Analysis of the phylogenetic tree showed that HaAPN1 and HaAPN3 are more closely related to each other than to other HaAPNs , and multiple circular transcripts were detected for these two genes. Therefore, we investigated whether the formation of circRNA derived from HaAPNs was evolutionarily conserved in Lepidoptera.…”

Section: Resultsmentioning

confidence: 99%

“…Therefore, we investigated whether the formation of circRNA derived from HaAPNs was evolutionarily conserved in Lepidoptera. For this investigation, we selected the PxAPN3 gene because HaAPN3 is more closely related to PxAPN3 than to HaAPN1 and because circular isoforms were also detected for this gene. Five circular isoforms were identified, and similar noncanonical splice signals also guided the formation of circRNA in PxAPN3 (Figs and ).…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Newly identified APN splice isoforms suggest novel splicing mechanisms may underlie circRNA circularization in moth

et al. 2019

View full text Add to dashboard Cite

Circular RNA (circ RNA ) have long been considered by‐products of splicing errors, but the coordination of RNA transcription and exon circularization events remains poorly understood. Here, we investigated this question using genes encoding aminopeptidases N ( APN s), which are receptors of Bacillus thuringiensis toxins, in the cotton bollworm, Helicoverpa armigera . We cloned and sequenced the cDNA of ten APN genes ( Ha APN 1‐10 ) located in the same APN gene cluster, and detected 20 and 14 novel splicing isoforms with exon skipping in Ha APN 1 and Ha APN 3 , respectively, whereas no or very few variants were found in the remaining genes. Further study identified 14 and 6 circular RNA (circ RNA ) in Ha APN 1 and Ha APN 3, respectively. Neither novel splicing isoforms nor circ RNA were detected in Ha APN 2 and Ha APN 5 . Distinct from the conventional GT / AG splicing signal, short co‐directional repeats were involved in the splicing of the linear and circular isoforms of Ha APN 1 and Ha APN 3 . Identification of the splice sites revealed that the linear isoforms may be related in some way to the circularization. Moreover, phylogenetic analysis and detection of circ RNA of the APN gene of the diamondback moth, Plutella xylostella ( Px APN 3 ), showed that circ RNA formation is relatively conserved during the lepidopteran evolutionary process. These results contribute to an improved understanding of lepidopteran APN s and this novel class of insect circ RNA .

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Newly identified APN splice isoforms suggest novel splicing mechanisms may underlie circRNA circularization in moth

et al. 2019

View full text Add to dashboard Cite

show abstract

“…(Jeong et al, 2006). Silkworms have 16 APN genes (Lin et al, 2014). Determining if the BmAPN4 5UI influences the promoters of other BmAPNs requires further experiments.…”

Section: Discussionmentioning

confidence: 99%

“…P2P has high activity only in the anterior midgut of P2 transgenic silkworms (Jiang et al, 2013a). BGIBMGA008060 is an aminopeptidase N (APN) and is named BmAPN4 (Lin et al, 2014;Nakanishi et al, 2002). Bioinformatic analysis showed a 475 bp 5UI upstream of BmAPN4.…”

Section: Introductionmentioning

confidence: 99%

The 5′-UTR intron of the midgut-specific BmAPN4 gene affects the level and location of expression in transgenic silkworms

Jiang

Huang

Sun

et al. 2015

Insect Biochemistry and Molecular Biology

Self Cite

View full text Add to dashboard Cite

“…Infection with B. bombysepticus , a main bacterial pathogen of the silkworm, leads to the classical symptoms of black chest septicemia: a peutz appears on the thoracoabdominal region or the 1st to 3rd segment of the abdomen and then expands to the whole body. Previous experiments demonstrated that many genes and pathways of the silkworm are modulated after B. bombysepticus infection (2, 3). However, little is known about the pathogenesis of B. bombysepticus (4).…”

Section: Genome Announcementmentioning

confidence: 99%

Complete Genome Sequence of Bacillus bombysepticus , a Pathogen Leading to Bombyx mori Black Chest Septicemia

et al. 2014

Self Cite

View full text Add to dashboard Cite

show abstract

Structural, evolutionary and functional analysis of APN genes in the Lepidoptera Bombyx mori

Cited by 20 publications

References 43 publications

Newly identified APN splice isoforms suggest novel splicing mechanisms may underlie circRNA circularization in moth

Newly identified APN splice isoforms suggest novel splicing mechanisms may underlie circRNA circularization in moth

The 5′-UTR intron of the midgut-specific BmAPN4 gene affects the level and location of expression in transgenic silkworms

Complete Genome Sequence of Bacillus bombysepticus , a Pathogen Leading to Bombyx mori Black Chest Septicemia

Contact Info

Product

Resources

About