Complete Genome Sequence of
            <i>Bacillus bombysepticus</i>
            , a Pathogen Leading to Bombyx mori Black Chest Septicemia

Cheng, Tingcai; Lin, Ping; Jin, Shengkai; Wu, Yuqian; Fu, Bohua; Long, Rodney; Liu, Duolian; Guo, Youbing; Li, Peng; Xia, Qingyou

doi:10.1128/genomea.00312-14

Cited by 18 publications

(9 citation statements)

References 2 publications

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“…An interesting outlier in our analysis was isolate D13, which shares a close relationship with B. bombysepticus strain Wang. This strain has been linked to Bombyx mori black chest septicemia [49], but additional comparative genomic studies of isolate D13 are necessary to understand its metabolic capabilities. Consistent with other studies demonstrating insertions and deletions in the genomes of various B. cereus strains [13][14][15]20,21], our studies provide evidence for recombination events leading to insertions and deletions which may be relevant both to the potential pathogenicity of these species as contaminants of bee pollen and to the evolutionary history of these lineages within the B. cereus group.…”

Section: Discussionmentioning

confidence: 99%

Phylogenetic Analysis of Bacillus cereus sensu lato Isolates from Commercial Bee Pollen Using tRNACys-PCR

et al. 2020

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Endospore-forming bacteria related to the Bacillus cereus group produce toxins that cause illnesses in organisms from invertebrates to mammals, including foodborne illnesses in humans. As commercial bee pollen can be contaminated with these bacteria, a comprehensive microbiological risk assessment of commercial bee pollen must be incorporated into the relevant regulatory requirements, including those that apply in Mexico. To facilitate detection of members of this group of bacteria, we have developed a PCR strategy that is based on the amplification of the single-copy tRNACys gene and specific genes associated with tRNACys to detect Bacillus cereus sensu lato (B. cereus s.l.). This tRNACys-PCR-based approach was used to examine commercial bee pollen for endospore-forming bacteria. Our analysis revealed that 3% of the endospore-forming colonies isolated from a commercial source of bee pollen were related to B. cereus s.l., and this result was corroborated by phylogenetic analysis, bacterial identification via MALDI-TOF MS, and detection of enterotoxin genes encoding the HBL and NHE complexes. The results show that the isolated colonies are closely related phylogenetically to B. cereus, B. thuringiensis, and B. bombysepticus. Our results indicate that the tRNACys-PCR, combined with other molecular tools, will be a useful approach for identifying B. cereus s.l. and will assist in controlling the spread of potential pathogens.

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Section: Discussionmentioning

confidence: 99%

Phylogenetic Analysis of Bacillus cereus sensu lato Isolates from Commercial Bee Pollen Using tRNACys-PCR

et al. 2020

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“…also comprises bacteria of minor medical and/or economic significance, such as Bacillus mycoides and Bacillus pseudomycoides 3 , Bacillus weihenstephanensis 4 , Bacillus toyonensis 5 , and Bacillus cytotoxicus 6 . Recently, Bacillus manliponensis 7 , Bacillus gaemokensis 8 , Bacillus bombysepticus 9 , Bacillus bingmayongensis 10 , Bacillus sp. 7_6_55CFAA_CT2 11 , and Bacillus wiedmannii 12 , have been recognized as plausible members of the group.…”

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confidence: 99%

Ribosomal background of the Bacillus cereus group thermotypes

Fiedoruk

Drewnowska

Daniluk

et al. 2017

Sci Rep

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In this study we reconstructed the architecture of Bacillus cereus sensu lato population based on ribosomal proteins, and identified a link between the ribosomal proteins’ variants and thermal groups (thermotypes) of the bacilli. The in silico phyloproteomic analysis of 55 ribosomal proteins (34 large and 21 small subunit r-proteins) of 421 strains, representing 14 well-established or plausible B. cereus sensu lato species, revealed several ribosomal clusters (r-clusters), which in general were well correlated with the strains’ affiliation to phylogenetic/thermal groups I–VII. However, a conformity and possibly a thermal characteristic of certain phylogenetic groups, e.g. the group IV, were not supported by a distribution of the corresponding r-clusters, and consequently neither by the analysis of cold-shock proteins (CSPs) nor by a content of heat shock proteins (HSPs). Furthermore, a preference for isoleucine and serine over valine and alanine in r-proteins along with a lack of HSP16.4 were recognized in non-mesophilic thermotypes. In conclusion, we suggest that the observed divergence in ribosomal proteins may be connected with an adaptation of B. cereus sensu lato members to various thermal niches.

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“…To investigate the molecular mechanisms underlying the regulation of bacteria toxin production by Bb , we searched the genome sequence to identify Bb toxins responsible for Bb pathogenicity. The complete Bb sequence was obtained from high-throughput Solexa paired-end sequences [ 13 ]. We indentified a predicted extracellular protein that was named Bb α-toxin.…”

Section: Resultsmentioning

confidence: 99%

Bacillus bombysepticus α-Toxin Binding to G Protein-Coupled Receptor Kinase 2 Regulates cAMP/PKA Signaling Pathway to Induce Host Death

Lin

Cheng

et al. 2016

PLoS Pathog

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Bacterial pathogens and their toxins target host receptors, leading to aberrant behavior or host death by changing signaling events through subversion of host intracellular cAMP level. This is an efficient and widespread mechanism of microbial pathogenesis. Previous studies describe toxins that increase cAMP in host cells, resulting in death through G protein-coupled receptor (GPCR) signaling pathways by influencing adenylyl cyclase or G protein activity. G protein-coupled receptor kinase 2 (GRK2) has a central role in regulation of GPCR desensitization. However, little information is available about the pathogenic mechanisms of toxins associated with GRK2. Here, we reported a new bacterial toxin-Bacillus bombysepticus (Bb) α-toxin that was lethal to host. We showed that Bb α-toxin interacted with BmGRK2. The data demonstrated that Bb α-toxin directly bound to BmGRK2 to promote death by affecting GPCR signaling pathways. This mechanism involved stimulation of Gαs, increase level of cAMP and activation of protein kinase A (PKA). Activated cAMP/PKA signal transduction altered downstream effectors that affected homeostasis and fundamental biological processes, disturbing the structural and functional integrity of cells, resulting in death. Preventing cAMP/PKA signaling transduction by inhibitions (NF449 or H-89) substantially reduced the pathogenicity of Bb α-toxin. The discovery of a toxin-induced host death specifically linked to GRK2 mediated signaling pathway suggested a new model for bacterial toxin action. Characterization of host genes whose expression and function are regulated by Bb α-toxin and GRK2 will offer a deeper understanding of the pathogenesis of infectious diseases caused by pathogens that elevate cAMP.

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Complete Genome Sequence of Bacillus bombysepticus , a Pathogen Leading to Bombyx mori Black Chest Septicemia

Cited by 18 publications

References 2 publications

Phylogenetic Analysis of Bacillus cereus sensu lato Isolates from Commercial Bee Pollen Using tRNACys-PCR

Phylogenetic Analysis of Bacillus cereus sensu lato Isolates from Commercial Bee Pollen Using tRNACys-PCR

Ribosomal background of the Bacillus cereus group thermotypes

Bacillus bombysepticus α-Toxin Binding to G Protein-Coupled Receptor Kinase 2 Regulates cAMP/PKA Signaling Pathway to Induce Host Death

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