Structural effects of titanium citrate on the human erythrocyte membrane

Suwalsky, Mario; Villena, Fernando Pardo-Manuel de; Norris, Beryl; Soto, Marco A.; Sotomayor, Carlos P.; Messori, Luigi; Zatta, Paolo

doi:10.1016/j.jinorgbio.2004.12.003

Cited by 24 publications

(22 citation statements)

References 21 publications

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“…In an attempt to further elucidate the effect of Cr(III) and Cr(VI) on cell membranes, the present work also examined their influence on the morphology of intact human erythrocytes, while isolated unsealed human erythrocyte membranes (IUM) were studied by fluorescence spectroscopy. These systems and techniques have been used in our laboratories to determine the interaction with and the membrane-perturbing effects of other inorganic compounds [17][18][19][20].…”

Section: Introductionmentioning

confidence: 99%

Cr(III) exerts stronger structural effects than Cr(VI) on the human erythrocyte membrane and molecular models

Suwalsky

Castro

Villena

et al. 2008

Journal of Inorganic Biochemistry

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Section: Introductionmentioning

confidence: 99%

Cr(III) exerts stronger structural effects than Cr(VI) on the human erythrocyte membrane and molecular models

Suwalsky

Castro

Villena

et al. 2008

Journal of Inorganic Biochemistry

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“…12,13 Titanium (IV) citrate (abbreviated as Ti (IV) -cit) can serve as a soluble precursor to titanium oxide materials via the Pechini process 14 and its structural effects have been studied on the human erythrocyte membrane, suggesting that disruption can be extended to other cells and affects its functions. 15 Paradies et al 8 reported that Ti (IV) citrate, which is known as a strong oxidant agent, is readily reduced to Ti (III) citrate in aqueous solution under UV radiation. In the form of Ti (III) citrate, it has been used extensively to probe redox reactions involving enzyme catalytic functions.…”

Section: Introductionmentioning

confidence: 99%

Mechanistic aspects of photocatalytic activity of metalloporphyrin–titanium mixtures in microemulsions

et al. 2016

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Photocatalytic activity of titanium citrate and its water-in-oil microemulsions mix with zinc (II) tetraphenylporphyrin have been studied by means of adding 4-chlorophenol and following its degradation using artificial ultraviolet and visible light. 4-Chlorophenol is degraded in 71 and 86%, respectively, in 30 min with the generation of various intermediaries, whose possible formation mechanisms have been proposed based in the results of this research. The generation of benzene derivatives, different to those usually reported for this type of treatment, suggests the possibility of controlling the generation of intermediaries by simply changing the type of surfactant. In the case discussed here, we have derived a simple method for isomer generation of trimethylbenzene using a model organohalogenated compound as water contaminant and cetyltrimethylammonium bromide as surfactant.

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“…These model systems and their interactions with the added substances have previously been used in the study of the effects of various compounds [17][18][19][20][21] including nanoparticles [11,[22][23][24][25][26] on cells and their membranes and were found to contribute to better understanding of the relevant mechanisms which are common in ex vivo and in vivo exposures.…”

Section: Introductionmentioning

confidence: 99%

Effect of carbon black nanomaterial on biological membranes revealed by shape of human erythrocytes, platelets and phospholipid vesicles

Pajnič¹,

Drašler²,

Šuštar³

et al. 2016

Nano Online

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BackgroundRecent advances in development and industrial production of nanomaterials require assessment of their effects on human and animal health. Toxicity of nanomaterials has therefore become an issue regarding the question which methods are the most appropriate for determining health risk [1,2]. Nanomaterial introduces also effects that cannot be explained by composition of the material and chemical reactions, but require consideration of non-specific properties, such as size and shape of particles, their electromagnetic properties and interactions with biological material [3]. Since the underlying mechanisms are largely unknown, standard methods for research, testing and safety are not necessarily relevant for these materials. Standard methods for research and testing of various compounds include experiments on experimental animals. These methods were found to have poor predictability regarding other species, in particular human, as shown by carcinogeneity studies [4][5][6]. It was suggested that in vitro research may provide essential information pertaining to the human health risks posed by nanoparticle exposure [7]. With fast and extensive development of new nanomaterials and their use in medicine and industry there is urgent need to develop effective and low cost methods that will enable understanding basic processes in living organisms. The role of nonspecific biophysical mechanisms has hitherto been underestimated as potentially essential in revealing biological processes but should be considered in future paradigms of research and testing of nanomaterials.Exogenously added substances first come in contact with cells by interacting with the membrane, so it is of great interest to study the interaction of these substances with biological membranes. Convenient systems for such studies are mammalian erythrocytes and giant unilamelar phospholipid vesicles (GUVs) composed of a closed bilayer membrane. These entities do not have internal structure (other than cortical membrane skeleton in case of erythrocytes) so their equilibrium shape is determined by the minimum of the membrane free energy [8]. Interaction of the added substance with the membrane causes changes in the membrane properties and in the constraints imposed upon the cell/vesicle (fixed area of the membrane, fixed difference between the areas of the two membrane layers, fixed cell/vesicle volume) which is reflected in the shape change [9]. Since mammalian erythrocytes and phospholipid vesicles (sized up to 100 micrometers) can be observed under the optical microscope, the effect of the exogenously added substances can be directly followed in real time.Further, of special interest are processes caused by exogenously added substances that increase the risk for thromboembolic events [ 10]. Some parameters of these processes, e.g. activation of platelets and coalescence of membranous structures due to the presence of nanomaterial have previously been studied [11].Membrane -nanomaterial interactions could be considered as one of the basic elements i...

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Structural effects of titanium citrate on the human erythrocyte membrane

Cited by 24 publications

References 21 publications

Cr(III) exerts stronger structural effects than Cr(VI) on the human erythrocyte membrane and molecular models

Cr(III) exerts stronger structural effects than Cr(VI) on the human erythrocyte membrane and molecular models

Mechanistic aspects of photocatalytic activity of metalloporphyrin–titanium mixtures in microemulsions

Effect of carbon black nanomaterial on biological membranes revealed by shape of human erythrocytes, platelets and phospholipid vesicles

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