Structural Effects of the Syntheticcobalt–Manganese-Zinc Ferrite Nanoparticles (Co0.3Mn0.2Zn0.5Fe2O4 NPs) on DNA and its Antiproliferative Effect on T47Dcells

Hekmat, Azadeh; Saboury, Ali Akbar

doi:10.1007/s12668-019-00657-5

Cited by 7 publications

(5 citation statements)

References 38 publications

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“…

\ln {K}_A=\frac{‐\varDelta {H}^{{}^{\circ}}}{R}\frac{1}{T}+\frac{\varDelta {S}^{{}^{\circ}}}{R}.

Consistent with the literature, if Δ S ° < 0, Δ H ° < 0, the hydrogen bond performs a main impact in the interaction and if Δ S ° > 0, Δ H ° > 0, the main force is hydrophobic interaction (Hekmat & Saboury, 2019). Accordingly, in HA–tyrosinase interaction, the positive values of Δ S ° and Δ H ° values show that the hydrophobic force performs a principal role (Hekmat & Saboury, 2019; Moosavi‐Movahedi et al, 2004).…”

Section: Resultssupporting

confidence: 75%

“…$$Consistent with the literature, if Δ S ° < 0, Δ H ° < 0, the hydrogen bond performs a main impact in the interaction and if Δ S ° > 0, Δ H ° > 0, the main force is hydrophobic interaction (Hekmat & Saboury, 2019). Accordingly, in HA–tyrosinase interaction, the positive values of Δ S ° and Δ H ° values show that the hydrophobic force performs a principal role (Hekmat & Saboury, 2019; Moosavi‐Movahedi et al, 2004). In FA–tyrosinase interaction, the negative Δ S ° and Δ H ° values show that the hydrogen bond performs the main effect.…”

Section: Resultssupporting

confidence: 60%

“…Utilizing K A , the absolute temperature ( T ), and the universal gas constant ( R ) the standard Gibbs free energy change (Δ G °) can be determined from Equation 7 (Ahmad et al, 2011; Hekmat & Saboury, 2019):

\varDelta {G}^{{}^{\circ}}=‐ RT\ln K

The magnitudes of Δ G ° were calculated at both temperatures for HA and FA (Table 1). The negative values of Δ G ° indicate that the binding of HA or FA to tyrosinase was spontaneous (Rahman et al, 2019).…”

Section: Resultsmentioning

confidence: 99%

“…The negative values of Δ G ° indicate that the binding of HA or FA to tyrosinase was spontaneous (Rahman et al, 2019). Employing Van't Hoff equation (Equation 8), the values of entropy change (Δ S °) and enthalpy change (Δ H °) were determined (Hekmat & Saboury, 2019).

$$ \ln {K}_A=\frac{‐\varDelta {H}^{{}^{\circ}}}{R}\frac{1}{T}+\frac{\varDelta {S}^{{}^{\circ}}}{R}.

…”

Section: Resultsmentioning

confidence: 99%

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Structural and inhibitory effects of fulvic and humic acids against tyrosinase

Taherkhani

Hekmat

Piri

et al. 2022

Journal of Food Biochemistry

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Inhibition of tyrosinase activity can control fruit browning and preserve the flavor and nutritional value of food. The impacts of fulvic acid (FA) and humic acid (HA) on tyrosinase activity were investigated utilizing circular dichroism (CD) and fluorescence spectroscopy, molecular docking (MD), and molecular dynamics simulations. HA and FA demonstrated a mixed type of inhibition with K i 2.02 and 5.2 μM, respectively. The thermodynamic parameters displayed that the hydrogen bond and hydrophobic force play a major role in the FA-tyrosinase and HA-tyrosinase interaction, respectively.Fluorescence experiments demonstrated changes in tyrosinase tertiary structures.HA could not destroy the tyrosinase secondary structure significantly, however, FA has a significant influence on the tyrosinase secondary structure. The molecular dynamics findings demonstrated the minimal fluctuations and the lowest flexibility in the complex amino acids in the HA-tyrosinase and FA-tyrosinase interaction. Altogether, HA and FA could be utilized in food industries as an accessible natural source for tyrosinase inhibition. Practical applicationsRecently, the investigation of tyrosinase inhibitors from the biosphere for hindrance of undesired browning in the food industry has increased considerably. Mushroom tyrosinase is a suitable model for kinetic research owing to its availability as well as close conformational similarity to tyrosinase in a mammal. Natural sources and their effective compounds could have wonderful potential on tyrosinase activity and structure, thus, in this study, the interactions between tyrosinase and fulvic acid (FA) and Humic acid (HA) were investigated. Previously, it has been shown that HA and FA have antioxidant properties and they can improve the quality of food via retarding lipid oxidation. Altogether, further investigations are warranted to draw firm conclusions, HA and FA could be utilized in food industries not only as antioxidant agents but also as an accessible natural source for tyrosinase inhibition.

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“…

\ln {K}_A=\frac{‐\varDelta {H}^{{}^{\circ}}}{R}\frac{1}{T}+\frac{\varDelta {S}^{{}^{\circ}}}{R}.

Section: Resultssupporting

confidence: 75%

Section: Resultssupporting

confidence: 60%

\varDelta {G}^{{}^{\circ}}=‐ RT\ln K

Section: Resultsmentioning

confidence: 99%

$$ \ln {K}_A=\frac{‐\varDelta {H}^{{}^{\circ}}}{R}\frac{1}{T}+\frac{\varDelta {S}^{{}^{\circ}}}{R}.

…”

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Structural and inhibitory effects of fulvic and humic acids against tyrosinase

Taherkhani

Hekmat

Piri

et al. 2022

Journal of Food Biochemistry

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“…Thus, to differentiate between apoptosis and necrosis, flow cytometry analysis was performed. In the flow cytometry assessment, PI is a dead cell marker and annexin V identifies apoptotic cells by binding externalized phosphatidylserine residues (41,42). Apoptosis serves as a critical mechanism to defend organs and tissues from numerous kinds of cell damage and stress.…”

Section: Discussionmentioning

confidence: 99%

The Impacts of Prepared Plasma-Activated Medium (PAM) Combined with Doxorubicin on the Viability of MCF-7 Breast Cancer Cells: A New Cancer Treatment Strategy

et al. 2022

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Background: For many years, the chemotherapeutic agent doxorubicin (DOX) has been used to treat various cancers; however, DOX initiates several critical adverse effects. Many studies have reported that non-thermal atmospheric pressure plasma can provide novel, but challenging, treatment strategies for cancer patients. To date, tissues and cells have been treated with plasma-activated medium (PAM) as a practical therapy. Consequently, due to the harmful adverse effects of DOX, we were motivated to elucidate the impact of PAM in the presence of DOX on MCF-7 cell proliferation. Methods: MTT assay, N-acetyl-L-cysteine (NAC) assay, and flow cytometry analysis were utilized in this research. Results:The results demonstrated that 0.45 µM DOX combined with 3-min PAM significantly induced apoptosis (p< 0.01) through intracellular ROS generation in MCF-7 when compared with 0.45 µM DOX alone or 3-min PAM alone. In contrast, after treatment with 0.45 µM DOX plus 4-min PAM, cell necrosis was increased. Hence, DOX combined with 4-min PAM has cytotoxic effects with different mechanisms than 4-min PAM alone, in which the number of apoptotic cells increases. Conclusions: Although further investigations are crucial, low doses of DOX plus 3-min PAM could be a promising strategy for cancer therapy. The findings from this research may offer advantageous and innovative clinical strategies for cancer therapy using PAM.

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