Structural determination of the K14 capsular polysaccharide from an ST25 Acinetobacter baumannii isolate, D46

Kenyon, Johanna J.; Hall, Ruth M.; Castro, Cristina De

doi:10.1016/j.carres.2015.09.002

Cited by 25 publications

(10 citation statements)

References 23 publications

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“…The variable region of some KL gene clusters, such as KL37 and KL14, lack genes for complex sugar synthesis, as they only contain simple sugars in their K units (Arbatsky et al, 2015; Kenyon et al, 2015). Also adding to their diversity, several KL regions contain redundant genes which are not required for the synthesis of the final K unit.…”

Section: Genetic Organization Of K Locimentioning

confidence: 99%

“…Variation between K unit structures may be subtle, for example, K12 and K13 differ only by the linkage of two glycans, which requires the use of an alternate Wzy polymerase; accordingly, the K loci of both strains are identical except for the wzy gene (Figure 2). Alternatively the variation may be striking, such as the incorporation of rare sugars including pseudaminic, legionaminic, or acinetaminic acid derivatives as seen in K2/6, K49, and K12/13 structures, respectively (Figure 2) (Kenyon et al, 2014a, 2015; Vinogradov et al, 2014; Kasimova et al, 2018). Interestingly, acinetaminic acid derivatives have only been identified in A. baumannii and are found nowhere else in nature (Kenyon et al, 2017).…”

Section: Cps Structuresmentioning

confidence: 99%

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Diversity and Function of Capsular Polysaccharide in Acinetobacter baumannii

2019

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The Gram-negative opportunistic bacterium Acinetobacter baumannii is a significant cause of hospital-borne infections worldwide. Alarmingly, the rapid development of antimicrobial resistance coupled with the remarkable ability of isolates to persist on surfaces for extended periods of time has led to infiltration of A. baumannii into our healthcare environments. A major virulence determinant of A. baumannii is the presence of a capsule that surrounds the bacterial surface. This capsule is comprised of tightly packed repeating polysaccharide units which forms a barrier around the bacterial cell wall, providing protection from environmental pressures including desiccation and disinfection regimes as well as host immune responses such as serum complement. Additionally, capsule has been shown to confer resistance to a range of clinically relevant antimicrobial compounds. Distressingly, treatment options for A. baumannii infections are becoming increasingly limited, and the urgency to develop effective infection control strategies and therapies to combat infections is apparent. An increased understanding of the contribution of capsule to the pathobiology of A. baumannii is required to determine its feasibility as a target for new strategies to combat drug resistant infections. Significant variation in capsular polysaccharide structures between A. baumannii isolates has been identified, with over 100 distinct capsule types, incorporating a vast variety of sugars. This review examines the studies undertaken to elucidate capsule diversity and advance our understanding of the role of capsule in A. baumannii pathogenesis.

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Section: Genetic Organization Of K Locimentioning

confidence: 99%

Section: Cps Structuresmentioning

confidence: 99%

Diversity and Function of Capsular Polysaccharide in Acinetobacter baumannii

2019

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“…In general, polysaccharides are obtained from animals (e.g., chondroitin sulfate, hyaluronic acid, chitosan, and heparin), microorganisms (e.g., xanthan gum, curdlan, gellan gum, and pullulan), and plants (e.g., carrageenan, guar gum, cellulose, and starch). − Among them, microorganism-secreted polysaccharides are preferable in terms of their simple purification process and high production rate. − …”

Section: Introductionmentioning

confidence: 99%

Macroporous Hydrogel Scaffolds with Tunable Physicochemical Properties for Tissue Engineering Constructed Using Renewable Polysaccharides

Zhang

et al. 2020

ACS Appl. Mater. Interfaces

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Polysaccharides have recently attracted increasing attention in the construction of hydrogel devices for biomedical applications. However, polysaccharide-based hydrogels are not suitable for most preclinical applications because of their limited mechanical properties and poor tunability. In this study, we employed a simple and eco-friendly approach to producing a macroporous polysaccharide hydrogel composed of salecan and κ-carrageenan without the use of toxic chemicals. We evaluated the physicochemical properties of the obtained salecan/κ-carrageenan hydrogel and found that its viscoelasticity, morphology, swelling, and thermal stability could be simply controlled by changing the polysaccharide dose in the pre-gel solution. The co-incubation of the fabricated hydrogel with mouse fibroblast cells demonstrated that the hydrogel can support cell adhesion, migration, and growth. Moreover, the hydrogel exhibited good biocompatibility in vivo. Overall, the findings provide a new strategy for the fabrication and optimization of polysaccharide-based hydrogel scaffolds for application in tissue engineering.

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“…The presence of a benzyloxy group at C-2 position and an pmethoxybenzyl (PMB) ether at C-3 position directed the formation of the 1,2-cis glycosidic linkage. [31] The presence of newly formed α-glycosidic linkage was confirmed by the NMR spectroscopic analysis [appearance of signals at δ 5.37 (d, J = 3.0 Hz, H-1 A ), 5.04 (d, J = 3.0 Hz, H-1 C ), 4.68 (d, J = 7.5 Hz, H-1 B ) in 1 H NMR and at δ 102.1 (C-1 B ), 99.3 (C-1 C ), 98.2 (C-1 A ) in 13 C NMR spectra]. In order to confirm the regioselective formation of (1!6)-α-glycosidic linkage, small amount of compound 9 was acetylated using acetic anhydride and pyridine.…”

Section: Resultsmentioning

confidence: 99%

Synthesis of the Pentasaccharide of the K14 Capsular Polysaccharide of Antibiotic Resistant ST25 Acinetobacter baumannii isolate, D46 and O‐Polysaccharide of Acinetobacter baumannii O11

Rana,

Kumar Misra

2024

ChemistrySelect

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Synthesis of the pentasaccharide corresponding to the K14 capsular polysaccharide from ST25 Acinetobacter baumannii isolate, D46 and O‐polysaccharide of Acinetobacter baumannii O11 has been achieved in very good yield using a convergent stereoselective [3+2] block glycosylation strategy. The synthetic scheme includes several regio‐ and stereose lective glycosylations as well as orthogonal glycosylation with high yield. Thioglycosides have been used as glycosyl donors using a combination of copper(II) bromide and tetra‐n‐butylammonium bromide (TBAB) as thiophilic activator for 1,2‐cis glycosylation and a combination of N‐iodosuccinimide (NIS) and trimethylsilyl trifluoromethanesulfonate (TMSOTf) as thiophilic activator for 1,2‐trans glycosylation. Glycosyl trichloroacetimidate derivative was used as glycosyl donor using perchloric acid supported on silica (HClO4–SiO2) as activator. A catalytic transfer hydrogenation reaction has been carried out for the removal of benzyloxy and benzylidene functional groups using triethylsilane as hydrogen source.

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Structural determination of the K14 capsular polysaccharide from an ST25 Acinetobacter baumannii isolate, D46

Cited by 25 publications

References 23 publications

Diversity and Function of Capsular Polysaccharide in Acinetobacter baumannii

Diversity and Function of Capsular Polysaccharide in Acinetobacter baumannii

Macroporous Hydrogel Scaffolds with Tunable Physicochemical Properties for Tissue Engineering Constructed Using Renewable Polysaccharides

Synthesis of the Pentasaccharide of the K14 Capsular Polysaccharide of Antibiotic Resistant ST25 Acinetobacter baumannii isolate, D46 and O‐Polysaccharide of Acinetobacter baumannii O11

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