Macroporous Hydrogel Scaffolds with Tunable Physicochemical Properties for Tissue Engineering Constructed Using Renewable Polysaccharides

Qi, Xiao-Liang; Su, Ting; Zhang, Mingjie; Tong, Xianqin; Pan, Wenhao; Zeng, Qiankun; Zhou, Zaigang; Shen, Liangliang; He, Xiaojun; Shen, Jianliang

doi:10.1021/acsami.9b20794

Cited by 80 publications

(36 citation statements)

References 42 publications

(65 reference statements)

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“…3 E, more typical F-actin fibers appeared in the cells adhered to APG3 compared with cells adhered to AHG. It was reported that integrins governed the cell adhesion, and increasing integrin aggregation promoted the formation of F-actin fibers [ 10 , 28 , 51 ]. As expected, cells cultured on APG3 displayed prominent integrin clustering, while the cells on AHG displayed low levels of integrin clustering ( Fig.…”

Section: Resultsmentioning

confidence: 99%

“…The mechanical parameters of hydrogel scaffolds will be sensed by cells and affect the cell behavior [ [6] , [7] , [8] ]. An idea hydrogel scaffold requires to match the tissue and ECM mechanics [ 9 , 10 , 11 ]. As living tissues and ECMs exhibit complex, time/rate dependent mechanical behaviors, it is essential to construct a hydrogel platform with adjustable mechanical strength.…”

Section: Introductionmentioning

confidence: 99%

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Mussel-inspired agarose hydrogel scaffolds for skin tissue engineering

Zhang

Zeng

et al. 2021

Bioactive Materials

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159

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Polysaccharide hydrogels are widely used in tissue engineering because of their superior biocompatibility and low immunogenicity. However, many of these hydrogels are unrealistic for practical applications as the cost of raw materials is high, and the fabrication steps are tedious. This study focuses on the facile fabrication and optimization of agarose-polydopamine hydrogel (APG) scaffolds for skin wound healing. The first study objective was to evaluate the effects of polydopamine (PDA) on the mechanical properties, water holding capacity and cell adhesiveness of APG. We observed that APG showed decreased rigidity and increased water content with the addition of PDA. Most importantly, decreased rigidity translated into significant increase in cell adhesiveness. Next, the slow biodegradability and high biocompatibility of APG with the highest PDA content (APG3) was confirmed. In addition, APG3 promoted full-thickness skin defect healing by accelerating collagen deposition and promoting angiogenesis. Altogether, we have developed a straightforward and efficient strategy to construct functional APG scaffold for skin tissue engineering, which has translation potentials in clinical practice.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Mussel-inspired agarose hydrogel scaffolds for skin tissue engineering

Zhang

Zeng

et al. 2021

Bioactive Materials

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159

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“…[ 24,25 ] A series of electrochemistry‐induced reactions resulted in two kinds of molecular interactions in the hydrogel model: one was coordination interaction of Fe 3+ ions with the sulfate groups of κ‐carrageenan; the other was from the molecular hydrogel‐bonding interactions between Fe 3+ ‐coordinated κ‐carrageenan and PAAm networks. [ 26,27 ] These two molecular interactions elicited functional improvements for the composite PAAm@κ‐carrageenan hydrogel in mechanical strength, interfacial adhesion, self‐healing, and surface patterning (Figure 1c).…”

Section: Resultsmentioning

confidence: 99%

Electrochemistry‐Induced Improvements of Mechanical Strength, Self‐Healing, and Interfacial Adhesion of Hydrogels

Yan

Zhang

2021

Advanced Materials

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physical/chemical functionalization is capable of optimization of mechanical properties, [7] interfacial adhesion, [8] and self-healing ability of hydrogels. [9] These protocols normally introduced functional elements or structures that elicited physical/chemical interactions for the performance improvements of hydrogels. For example, polymeric nanoparticles from crystallization-driven self-assembly enabled mechanical improvements on hydrogel by physical hybridization with matrix. [10] Grafting silica nanoparticles to a double-network polymer also improved the hydrogel mechanical performances. [11] Construction of electrostatic interaction between carboxyl groups and various surfaces resulted in a catechol-chemistrybased hydrogel for the long-term adhesiveness. [12] Treatment by metal ions resulted in a hydrophobic surface that promoted formation of a water-resistant molecular bridge between the hydrogel surface and hydrophobic domains on the substrates, endowing the hydrogels prominent underwater-adhesion capability. [13] Similar adhesive hydrogel could also be achieved by dopamine-modified clay nanosheets. [14] An effective molecular structure design based on acid-ether hydrogen bonding and imine bonds was capable of accelerating hydrogel healing time to 30 min. [15] A dynamic borate bond in network enabled 100% cure of hydrogel in air. [16] Reversible metal-ligand coordination bonding interaction could also be used to construct self-healing hydrogel. [17] These protocols are efficient for the construction of functional hydrogels, yet still challenging to synchronously improve the mechanical strength, self-healing, and interfacial adhesion of a hydrogel, and especially, hard to endow the hydrogel with modular sensitivity to external pressure. Therefore, the development of a new class of protocol is still essential.Herein, we proposed an electrochemistry functionalization protocol, in which the functional improvements on hydrogels were achieved by the electrode reactions, and electrochemistrytriggered ionic and molecular migration. This protocol was capable of enabling the function improvements of the hydrogel in mechanical strength, interfacial adhesion, and self-healing. In the meantime, it allowed generation of various patterns on the hydrogel surface, and endowed the hydrogel modular sensitivity to external pressure. The functional improvements Hydrogels have demonstrated great potential in biomedical and engineering areas. To improve the physical performance, development of efficient physical/chemical protocols is essential. Herein, an electrochemistry functionalization strategy that is capable of enabling the functional improvements of hydrogel is reported. The electrochemistry functionalization is demonstrated on a hydrogel model of polyacrylamide (PAAm)@κ-carrageenan. The electrochemistry reaction generates metal ions (Fe 3+ ) that migrate and coordinate with the sulfate groups of κ-carrageenan resulting in the prominent function improvements. In comparison with untreated PAAm@κcarrageenan hydrogel, it c...

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“…Hydrogels are three-dimensional networks composed of hydrophilic polymers cross-linked through covalent bonds or held together via physical intermolecular interactions [9,10]. Over the past few decades, methods for administering drugs via hydrogels have gained increasing attention, as regulating the rate of drug release with a controlled release; the mechanism offers numerous advantages over conventional dosage regimens [11,12].…”

Section: Introductionmentioning

confidence: 99%

Synthesis and Characterization of Lyophilized Chitosan-Based Hydrogels Cross-Linked with Benzaldehyde for Controlled Drug Release

Damiri

Bachra

Bounacir

et al. 2020

Journal of Chemistry

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In this study, chitosan-based hydrogels were produced by incorporating three drugs with a different solubility into a polymer matrix. The lyophilized chitosan salt was prepared using an innovative and less-expensive synthetic process by the freeze-drying technique. Firstly, the three drugs (caffeine, ascorbic acid, and 5-fluorouracil (5-FU)) were selected as model drugs to test the in vitro release behavior of the hydrogel. The drugs were solubilized in chitosan salt, lyophilized, and cross-linked with benzaldehyde involving the formation of a Schiff base with (–C=N-) linkage to produce a physical hydrogel. Subsequently, the physicochemical properties of N-benzyl chitosan and lyophilized chitosan salt were evaluated by Fourier-transform infrared (FTIR) spectra, scanning electron microscopy (SEM), and thermogravimetric analysis (TGA). The intrinsic viscosity of the conventional chitosan was determined by the Mark–Houwink–Sakurada equation. Moreover, the kinetics of hydrogel swelling and drug release were studied by the UV-visible method at physiological conditions (pH = 7.4 at 37°C). The results show that lyophilized N-benzyl chitosan had a maximum swelling ratio of 720 ± 2% by immersion in phosphate-buffered saline solutions (PBS) (pH = 7.4 at 37°C). In vitro drug releases were evaluated in PBS, and the obtained results show that the maximum drug release after 24 h was 42% for caffeine, 99% for 5-FU, and 94% for ascorbic acid. Then, to optimize the cumulative release of caffeine, Tween 20 was added and 98% as a release percentage was obtained. The drug-loading results were investigated with the Korsmeyer–Peppas kinetic model and applied to determine the drug release mechanism.

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Macroporous Hydrogel Scaffolds with Tunable Physicochemical Properties for Tissue Engineering Constructed Using Renewable Polysaccharides

Cited by 80 publications

References 42 publications

Mussel-inspired agarose hydrogel scaffolds for skin tissue engineering

Mussel-inspired agarose hydrogel scaffolds for skin tissue engineering

Electrochemistry‐Induced Improvements of Mechanical Strength, Self‐Healing, and Interfacial Adhesion of Hydrogels

Synthesis and Characterization of Lyophilized Chitosan-Based Hydrogels Cross-Linked with Benzaldehyde for Controlled Drug Release

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