Mussel-inspired agarose hydrogel scaffolds for skin tissue engineering

Abstract

Polysaccharide hydrogels are widely used in tissue engineering because of their superior biocompatibility and low immunogenicity. However, many of these hydrogels are unrealistic for practical applications as the cost of raw materials is high, and the fabrication steps are tedious. This study focuses on the facile fabrication and optimization of agarose-polydopamine hydrogel (APG) scaffolds for skin wound healing. The first study objective was to evaluate the effects of polydopamine (PDA) on the mechanical properties, water holding capacity and cell adhesiveness of APG. We observed that APG showed decreased rigidity and increased water content with the addition of PDA. Most importantly, decreased rigidity translated into significant increase in cell adhesiveness. Next, the slow biodegradability and high biocompatibility of APG with the highest PDA content (APG3) was confirmed. In addition, APG3 promoted full-thickness skin defect healing by accelerating collagen deposition and promoting angiogenesis. Altogether, we have developed a straightforward and efficient strategy to construct functional APG scaffold for skin tissue engineering, which has translation potentials in clinical practice.

Construction of heparin-based hydrogel incorporated with Cu5.4O ultrasmall nanozymes for wound healing and inflammation inhibition

“…Efficient reconstruction of the function and integrity of the damaged tissues is currently the central focus for their clinical treatment [ 3 ]. Multiple therapeutic options, including novel dressing products [ 4 , 5 ], growth factors [ 6 , 7 ], and bioengineered skin grafts [ 8 ] are available. However, strategies for the regenerative healing of the injured tissues, especially for chronic wounds, remain elusive.…”

A Prototype Skin Substitute, Made of Recycled Marine Collagen, Improves the Skin Regeneration of Sheep

“…Among them, the use of collagen-based biomaterials, being the main protein of the ECM, has been widely investigated for tissue engineering studies and showed promising results. As a matter of fact, the application of these biomaterials for the treatment of skin wounds has been reinforced for their characteristics such as biocompatibility [ 16 ], low immunogenicity [ 17 ], and bioactive properties [ 18 ].…”

Systematic Review: Adipose-Derived Mesenchymal Stem Cells, Platelet-Rich Plasma and Biomaterials as New Regenerative Strategies in Chronic Skin Wounds and Soft Tissue Defects

“…In the past few decades, numerous bi-layer dermal substitutes have been developed and applied for the management of full-thickness skin defects, such as Alloderm ® [13,14], Integra ® [15,16], Pelnac ® [17] and Lando ® , of which, the low-layer porous CSS function as DRT. Being the main protein of the extracellular matrix, collagen has excellent biocompatibility with low immunogenicity [18]. In addition, the porous structure guides inward proliferation and the migration of fibroblasts and endothelial cells, which promotes granulation tissue and angiogenesis for WH with less scarring [21,22].…”