Structural characterization of ulvans extracted from blade (Ulva ohnoi) and filamentous (Ulva tepida and Ulva prolifera) species of cultivated Ulva

Glasson, Christopher R. K.; Luiten, Cara A.; Carnachan, Susan; Daines, Alison M.; Kidgell, Joel T.; Hinkley, Simon F.R.; Praeger, Christina; Martinez, Maria Andrade; Sargison, Liam; Magnusson, Marie; Nys, Rocky de; Sims, Ian M.

doi:10.1016/j.ijbiomac.2021.11.100

Cited by 21 publications

(12 citation statements)

References 56 publications

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“…Besides, the connection manner of →4)- α -D-GalA p -(1→4)- α -D-GalA p -(1→ was speculated to exist through the HMBC correlation from C1 (δ 101.88) of H to H4 (δ 4.33) of F and C4 (δ 79.68) of F to H1 (δ 4.85) of H [ 47 , 48 ]. The correlation from C1 (δ 100.39) of F to H4 (δ 4.27) of E implied the presence of 4)- α -D-GalA p -(1→3,4)- α -D-Glc p -(1→ linkage [ 49 ]. The cross peaks between H1 (δ 5.08) of D and C4 (δ 78.18) of C, and H1 (δ 4.98) of G and C3 (δ 83.3) of E were observed in the HMBC spectrum, implying the presence of side chains of α -L-Ara f -(1→2,4)- α -L-Rha p -(1→ and α -L-Ara p -(1→3,4)-α-D-Glc p -(1→, respectively [ 32 , 50 ].…”

Section: Resultsmentioning

confidence: 99%

Isolation, Structural Characterization and Macrophage Activation Activity of an Acidic Polysaccharide from Raspberry Pulp

et al. 2022

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The discovery of safe and effective plant polysaccharides with immunomodulatory effects has become a research hotspot. Raspberry is an essential commercial fruit and is widely distributed, cultivated, and consumed worldwide. In the present study, a homogeneous acidic polysaccharide (RPP-2a), with a weight-average molecular weight (Mw) of 55582 Da, was isolated from the pulp of raspberries through DEAE-Sepharose Fast Flow and Sephadex G-200 chromatography. RPP-2a consisted of rhamnose, arabinose, galactose, glucose, xylose, galacturonic acid and glucuronic acid, with a molar ratio of 15.4:9.6:7.6:3.2:9.1:54.3:0.8. The results of Fourier transform infrared spectroscopy (FT-IR), gas chromatography-mass spectrometer (GC-MS), 1D-, and 2D-nuclear magnetic resonance (NMR) analyses suggested that the backbone of RPP-2a was primarily composed of →2)-α-L-Rhap-(1→, →2,4)-α-L-Rhap-(1→, →4)-α-D-GalAp-(1→, and →3,4)-α-D-Glcp-(1→ sugar moieties, with side chains of α-L-Araf-(1→, α-L-Arap-(1→, and β-D-Galp-(1→3)-β-D-Galp-(1→ residues linked to the O-4 band of rhamnose and O-3 band of glucose residues. Furthermore, RPP-2a exhibited significant macrophage activation activity by increasing the production of nitric oxide (NO), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-1β (IL-1β), and the expression of inducible nitric oxide synthase (iNOS) and cytokines at the transcriptional level in RAW264.7 cells. Overall, the results indicate that RPP-2a can be utilized as a potential natural immune-enhancing agent.

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Section: Resultsmentioning

confidence: 99%

Isolation, Structural Characterization and Macrophage Activation Activity of an Acidic Polysaccharide from Raspberry Pulp

et al. 2022

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“…The shifts of H1–H5 and C1–C6 were obtained from the COSY spectrum and HSQC spectrum, respectively ( Figure 3 A,B). In addition, a C-6 shift was at around 175.41 ppm, and a C-4 shift at 78.40 ppm was a down-shift, which implied residue Ⅲ was 1,4-β-D-GlcpA [ 26 , 28 , 29 ].…”

Section: Resultsmentioning

confidence: 99%

“…Of interest, from the 13 C NMR, 1 H NMR, COSY NMR, and HSQC NMR of HU, we found some anomeric signals, and the chemical shifts of H/C were at 5.19/95.99, 4.91/98.13, 4.76/100.71, 5.11/98.13, 4.66/104.03, 4.85/103.10, and 5.15/100.24 ppm. Based on published literature and our results of the NMRs of U, we assigned the anomeric signals at 5.19/95.99, 4.91/98.13, 4.76/100.71, 5.11/98.13, 4.66/104.03, and 4.85/103.10 ppm to sugar residues Ⅰ (1,3-α-L-Rhap), Ⅱ (1,4-α-L-Rhap3S), Ⅲ (1,4-β-D-GlcpA), Ⅳ (1,4-β-D-Glcp), Ⅴ (1,4-β-D-Glcp3Me), and Ⅷ (1,4-α-L-IdopA) in HU [ 26 , 27 , 28 , 29 , 31 , 32 ]. Chemical shifts of other protons and carbon atoms of sugar residues were identified by analyzing the COSY and HSQC Ns of HU and listed in Table 2 .…”

Section: Resultsmentioning

confidence: 99%

Structural Characterization and Anti-Nonalcoholic Fatty Liver Effect of High-Sulfated Ulva pertusa Polysaccharide

et al. 2022

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The high-sulfated derivative of Ulva pertusa polysaccharide (HU), with unclear structure, has better anti-hyperlipidmia activity than U pertusa polysaccharide ulvan (U). In this study, we explore the main structure of HU and its therapeutic effect against nonalcoholic fatty liver disease (NAFLD). The main structure of HU was elucidated using FT-IR and NMR (13C, 1H, COSY, HSQC, HMBC). The anti-NAFLD activity of HU was explored using the high-fat diet mouse model to detect indicators of blood lipid and liver function and observe the pathologic changes in epididymal fat and the liver. Results showed that HU had these main structural fragments: →4)-β-D-Glcp(1→4)-α-L-Rhap2,3S(1→; →4)-α-L-Rhap3S(1→4)-β-D-Xylp2,3S(1→; →4)-α-L-Rhap3S(1→4)-β-D-Xylp(1→; →4)-α-L-IdopA3S(1→4)-α-L-Rhap3S(1→; →4)-β-D-GlcpA(1→3)-α-L-Rhap(1→; →4)-α-L-IdopA3S(1→4)-β-D-Glcp3Me(1→; →4)-β-D-Xylp2,3S(1→4)-α-L-IdopA3S(1→; and →4)-β-D-Xylp(1→4)-α-L-IdopA3S(1→. Treatment results indicated that HU markedly decreased levels of TC, LDL-C, TG, and AST. Furthermore, lipid droplets in the liver were reduced, and the abnormal enlargement of epididymal fat cells was suppressed. Thus, HU appears to have a protective effect on the development of NAFLD.

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“…However, the selection of pore size for ultrafiltration is not only based on the molecular size of ulvan but also depends on the elution amount (which decreasing with the pore size). In order to balance the influence of elution amount and pore size, the MWCO of about 10 kDa was generally selected (Glasson et al 2022(Glasson et al , 2017.…”

Section: Purification Methods Of Ulvanmentioning

confidence: 99%

Ulvan and Ulva oligosaccharides: a systematic review of structure, preparation, biological activities and applications

Li,

Tang,

et al. 2023

Bioresour. Bioprocess.

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Ulva is one of the main green algae causing green tide disasters. Ulvan is the primarily component polysaccharide of the cell wall of Ulva and its complex structure and monosaccharide composition resulted in various biological activities. However, the high-value and effective utilization of extracted ulvan have been obstructed by limitations ranging from large molecular weight and low solubility to poor bioavailability. Ulva oligosaccharide obtained by degrading ulvan can not only ideally retain the various biological activities of ulvan very well but also effectively solve the problems of low solubility and poor bioavailability. The preparation and biological activity studies of ulvan and Ulva oligosaccharides have become a hot spot in the field of marine biological resources development research. At present, the comprehensive reviews of ulvan and Ulva oligosaccharides are still scarce. What are overviewed in this paper are the chemical composition, structure, extraction, and purification of ulvan and Ulva oligosaccharides, where research progress on the biological activities of ulvan and Ulva oligosaccharides is summarized and prospected. A theoretical and practical basis has been provided for further research on ulvan and Ulva oligosaccharides, as well as the high-value development and effective utilization of marine algae resources. Graphical Abstract

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Structural characterization of ulvans extracted from blade (Ulva ohnoi) and filamentous (Ulva tepida and Ulva prolifera) species of cultivated Ulva

Cited by 21 publications

References 56 publications

Isolation, Structural Characterization and Macrophage Activation Activity of an Acidic Polysaccharide from Raspberry Pulp

Isolation, Structural Characterization and Macrophage Activation Activity of an Acidic Polysaccharide from Raspberry Pulp

Structural Characterization and Anti-Nonalcoholic Fatty Liver Effect of High-Sulfated Ulva pertusa Polysaccharide

Ulvan and Ulva oligosaccharides: a systematic review of structure, preparation, biological activities and applications

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