Structural characterization of polysaccharide from Centipeda minima and its hypoglycemic activity through alleviating insulin resistance of hepatic HepG2 cells

Chen, Jun-Cheng; Li, Lin; Zhang, Xia; Wan, Liting; Zheng, Qingsong; Xu, Dan; Li, Yuting; Lv, Yi; Chen, Meisi; Li, Bing; Chen, Zhiyi

doi:10.1016/j.jff.2021.104478

Cited by 29 publications

(13 citation statements)

References 61 publications

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“…The apparatus was equipped with a HP5-ms quartz capillary column, and the column size was 30 m × 0.25 mm × 0.25 µm. Experiment parameters were based on the literatures (28,29). Set the temperature to 80 • C and hold for 3 min, heat to 250 • C at a rate of 10 • C/min, and then hold for 10 min; carrier gas was helium (1 mL/min); injection temperature was 250 • C, and injection volume was 1 µL.…”

Section: Methylation and Gc-ms Of Polysaccharidesmentioning

confidence: 99%

“…The 1 H and 13 C NMR spectroscopy, HSQC (heteronuclear single-quantum coherence), 1 H-1 H COSY (correlation spectroscopy), and HMBC (heteronuclear multiple-bond correlation) spectroscopy were observed by a Bruker ARX500 NMR spectrometer (Brucker, Rheinstetten, Germany) at 297.3 K according to previous report (28). Sample (50 mg) was dissolved in deuterated water (D 2 O, 99.9%, Sigma, United States) and freeze-dried and repeated several times to completely replace the H 2 O by D 2 O.…”

Section: Nmr Spectroscopy Analysesmentioning

confidence: 99%

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Characterization of Structure and Antioxidant Activity of Polysaccharides From Sesame Seed Hull

et al. 2022

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Sesame seed hull is the major by-product of sesame seed processing and is rich in polysaccharides. In this work, sesame hull polysaccharides (SHP) were extracted by ultrasound-assisted alkali extraction methods with a yield of 6.49%. Three purified polysaccharide fractions were obtained after decolorization, deproteinization, and column chromatography. Then, their main composition and antioxidant activity were investigated. The dominant fraction was SHP-2 with a yield of 3.78%. It was composed of galacturonic acid (51.3%), glucuronic acid (13.8%), rhamnose (8.9%), glucose (8.4%), and others. The linkage types of SHP-2 have the α-D-GalpA-(1,4)-linked, α-D-GlcpA-(1,2)-linked, β-T-D-Rhap-linked, β-D-Glcp-(1,6)-linked, β-T-D-Galp-linked, α-L-Xylp-(1,4)-linked, α-L-Araf-(1,3,5)-linked, and β-D-Manp-(1,4)-linked. This study might provide some useful basic data for developing applications for sesame seed hull polysaccharides in the food and pharmaceutical industries.

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Section: Methylation and Gc-ms Of Polysaccharidesmentioning

confidence: 99%

Section: Nmr Spectroscopy Analysesmentioning

confidence: 99%

Characterization of Structure and Antioxidant Activity of Polysaccharides From Sesame Seed Hull

et al. 2022

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“…The biological activities of polysaccharides have become research hotspots [ 27 ]. Plant polysaccharides have biological activities, such as immune regulation, antiviral, hypoglycemic, hypolipidemic, and antioxidation effects, which participate in various physiological metabolisms of the human body [ 28 ]. IOP was an acidic heteropolysaccharide with a rough surface.…”

Section: Discussionmentioning

confidence: 99%

Lipid-Lowering Effects of Inonotus obliquus Polysaccharide In Vivo and In Vitro

Yang

Cui

et al. 2021

Foods

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Excessive lipid intake will cause hyperlipidemia, fatty liver metabolism disease, and endanger people’s health. Edible fungus polysaccharide is a natural active substance for lipid lowering. In this study, the HepG2 cell model induced by oleic acid and mice model induced by a high-fat diet was established. The lipid-lowering effects of Inonotus obliquus polysaccharide (IOP) was investigated in vivo and in vitro. Glucose (251.33 mg/g), rhamnose (11.53 mg/g), ribose (5.10 mg/g), glucuronic acid (6.30 mg/g), and galacturonic acid (2.95 mg/g) are present in IOP, at a ratio of 85.2:3.91:1.73:2.14:1. The molecular weight of IOP is 42.28 kDa. Treatment with 60 mg/L of IOP showed a significant lipid-lowering effect in HepG2 cells compared with the oleic acid-treated group. In the oil red O-stained images, the red fat droplets in the IOP-treated groups were significantly reduced. TC and TG levels of IOP-treated groups decreased. IOP can alleviate the lipid deposition in the mice liver due to high-fat diet, and significantly reduce their serum TC, TG, and LDL-C contents. IOP could activate AMPK but decrease the SREBP-1C, FAS, and ACC protein expression related to adipose synthesis in mice. IOP has a certain potential for lipid-lowering effects both in vivo and in vitro.

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“…However, no phytochemical research on Chieh qua was reported before. Natural polysaccharides have attracted more and more researchers' interest because of their various biological activities (Chen et al, 2021;He et al, 2020;Huang et al, 2015;Li et al, 2020Yu et al, 2021). Here we reported the antioxidant activities of water-soluble polysaccharide extracted from Chieh qua.…”

Section: Introductionmentioning

confidence: 92%

“…ABTS free radicals scavenging ability of BHCP was determined in terms of the method conducted before (Chen et al, 2021;Hu et al, 2021) with some modifications. ABTS radicals cation was prepared by mixing 7 mmol /L ABTS with 2.45 mmol/L potassium persulphate in the ratio of 1:1 and incubated at room temperature in darkroom for 16 h. The solution was then diluted with phosphate buffer (10 mmol/L, pH 7.4) to an absorbance of 0.70 ± 0.02 at 734 nm.…”

Section: Abts Free Radicals Scavenging Activitymentioning

confidence: 99%

Characterization and antioxidant activities of polysaccharide extracted from Benincasa hispida var. chieh-qua How

et al. 2022

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The water-soluble polysaccharide (BHCP) was isolated from the hot aqueous extract of Chieh qua (Benincasa hispida var. chieh-qua How) fruit. The polysaccharide was found to contain D-galactose and D-glucose in a molar ratio of 2.59:1 with both α-and β-glycosidic bond. The strong free radicals scavenging activity on DPPH, ABTS, and hydroxyl radicals of the BHCP was demonstrated, and showing the BHCP from Chieh qua has potential application value on the functional food.

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Structural characterization of polysaccharide from Centipeda minima and its hypoglycemic activity through alleviating insulin resistance of hepatic HepG2 cells

Cited by 29 publications

References 61 publications

Characterization of Structure and Antioxidant Activity of Polysaccharides From Sesame Seed Hull

Characterization of Structure and Antioxidant Activity of Polysaccharides From Sesame Seed Hull

Lipid-Lowering Effects of Inonotus obliquus Polysaccharide In Vivo and In Vitro

Characterization and antioxidant activities of polysaccharide extracted from Benincasa hispida var. chieh-qua How

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