Structural characterization of novel extracellular liamocins (mannitol oils) produced by Aureobasidium pullulans strain NRRL 50380

Price, Neil P. J.; Manitchotpisit, Pennapa; Vermillion, Karl E.; Bowman, Michael J.; Leathers, Timothy D.

doi:10.1016/j.carres.2013.01.014

Cited by 80 publications

(70 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Similar structural motifs have previously been reported in the literature from endophytic, and marine fungal organisms, including a trimeric dihydroxydecanoic acid with antibiotic activity against Gram-positive bacteria (Gaskins and Cheung 1986; Cheikh-Ali et al 2015; Price et al 2013; Bischoff et al 2015; Kim et al 2015). Our MS based chemical survey also revealed other members of that molecular family containing four and six repeats, which represent additional polymeric products (Fig.…”

Section: Resultssupporting

confidence: 80%

A metabolomics guided exploration of marine natural product chemical space

et al. 2016

View full text Add to dashboard Cite

Introduction Natural products from culture collections have enormous impact in advancing discovery programs for metabolites of biotechnological importance. These discovery efforts rely on the metabolomic characterization of strain collections. Objective Many emerging approaches compare metabolomic profiles of such collections, but few enable the analysis and prioritization of thousands of samples from diverse organisms while delivering chemistry specific read outs. Method In this work we utilize untargeted LC–MS/MS based metabolomics together with molecular networking to Result This approach annotated 76 molecular families (a spectral match rate of 28 %), including clinically and biotechnologically important molecules such as valinomycin, actinomycin D, and desferrioxamine E. Targeting a molecular family produced primarily by one microorganism led to the isolation and structure elucidation of two new molecules designated maridric acids A and B. Conclusion Molecular networking guided exploration of large culture collections allows for rapid dereplication of know molecules and can highlight producers of uniques metabolites. These methods, together with large culture collections and growing databases, allow for data driven strain prioritization with a focus on novel chemistries.

show abstract

Section: Resultssupporting

confidence: 80%

A metabolomics guided exploration of marine natural product chemical space

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Not only numerous hydrolytic enzymes [58], but also various antimicrobial compounds, such as exophilin A [12], siderophores [59] and aureobasidin A [10], might have roles in the strong antagonistic effects of A. pullulans towards other species.…”

Section: Resultsmentioning

confidence: 99%

“…Due to its strong antagonistic activity against other microorganisms, A. pullulans is used as a biocontrol agent in agriculture [11]. Additionally, a recent study reported that some strains of A. pullulans can produce an antibacterial compound, exophilin A, as well as high yields of liamocins, and heavy oils with previously unknown acylated mannitol structures, which have possible industrial applications as surfactants [12]. …”

Section: Introductionmentioning

confidence: 99%

Genome sequencing of four Aureobasidium pullulans varieties: biotechnological potential, stress tolerance, and description of new species

et al. 2014

View full text Add to dashboard Cite

BackgroundAureobasidium pullulans is a black-yeast-like fungus used for production of the polysaccharide pullulan and the antimycotic aureobasidin A, and as a biocontrol agent in agriculture. It can cause opportunistic human infections, and it inhabits various extreme environments. To promote the understanding of these traits, we performed de-novo genome sequencing of the four varieties of A. pullulans.ResultsThe 25.43-29.62 Mb genomes of these four varieties of A. pullulans encode between 10266 and 11866 predicted proteins. Their genomes encode most of the enzyme families involved in degradation of plant material and many sugar transporters, and they have genes possibly associated with degradation of plastic and aromatic compounds. Proteins believed to be involved in the synthesis of pullulan and siderophores, but not of aureobasidin A, are predicted. Putative stress-tolerance genes include several aquaporins and aquaglyceroporins, large numbers of alkali-metal cation transporters, genes for the synthesis of compatible solutes and melanin, all of the components of the high-osmolarity glycerol pathway, and bacteriorhodopsin-like proteins. All of these genomes contain a homothallic mating-type locus.ConclusionsThe differences between these four varieties of A. pullulans are large enough to justify their redefinition as separate species: A. pullulans, A. melanogenum, A. subglaciale and A. namibiae. The redundancy observed in several gene families can be linked to the nutritional versatility of these species and their particular stress tolerance. The availability of the genome sequences of the four Aureobasidium species should improve their biotechnological exploitation and promote our understanding of their stress-tolerance mechanisms, diverse lifestyles, and pathogenic potential.Electronic supplementary materialThe online version of this article (doi:10.1186/1471-2164-15-549) contains supplementary material, which is available to authorized users.

show abstract

“…Generally, A. pullulans produces polysaccharides, including pullulan and β-glucan, which find industrial and medical applications (Yurlova and de Hoog, 1997;Cheng et al, 2011;Muramatsu et al, 2012). Recently, A. pullulans has been shown to produce (poly)malic acid (Nagata et al, 1993), lipase , laccase (Rich et al, 2011), mannitol oils (Price et al, 2013), biocontrol agents (Mari et al, 2012), biosurfactants , valuable lipids (Turk et al, 2004), and siderophores (Ma et al, 2012). A. pullulans from plant flowers have also been found to produce several biosurfactants, depending on their phylogenetic class (unpublished data).…”

Section: Resultsmentioning

confidence: 99%

Yeasts in the Flowers of Wild Fleabane [Erigeron annus (L.) Pers.]

Kim

2015

Korean Journal of Environmental Agriculture

View full text Add to dashboard Cite

BACKGROUND:Yeasts associated with fleabane flowers were identified using isolation methods previously applied in yeast biotechnology. A culture-based approach was required for isolation of many yeast strains associated with fleabane. METHODS AND RESULTS:We spread homogenized fleabane flowers onto GPY medium containing chloramphenicol, streptomycin, Triton X-100, and Lsorbose. We isolated 79 yeast strains from the flowers of wild fleabane, and identified the yeasts via phylogenetic analysis of isolates from agar plates. The yeast species included 39 isolates of Aureobasidium pullulans, 17 of the genus Candida, 14 of the genus Rhodosporidium, 6 of the genus Cryptococcus, and 3 of the genus Rhodotorula. CONCLUSION: Yeast isolates associated with fleabane flowers included A. pullulans (39 isolates) and other yeast species (40 isolates). Such yeast isolates may have biotechnological potential.

show abstract

Structural characterization of novel extracellular liamocins (mannitol oils) produced by Aureobasidium pullulans strain NRRL 50380

Cited by 80 publications

References 24 publications

A metabolomics guided exploration of marine natural product chemical space

A metabolomics guided exploration of marine natural product chemical space

Genome sequencing of four Aureobasidium pullulans varieties: biotechnological potential, stress tolerance, and description of new species

Yeasts in the Flowers of Wild Fleabane [Erigeron annus (L.) Pers.]

Contact Info

Product

Resources

About