Yeasts in the Flowers of Wild Fleabane [Erigeron annus (L.) Pers.]

Abstract

BACKGROUND:Yeasts associated with fleabane flowers were identified using isolation methods previously applied in yeast biotechnology. A culture-based approach was required for isolation of many yeast strains associated with fleabane. METHODS AND RESULTS:We spread homogenized fleabane flowers onto GPY medium containing chloramphenicol, streptomycin, Triton X-100, and Lsorbose. We isolated 79 yeast strains from the flowers of wild fleabane, and identified the yeasts via phylogenetic analysis of isolates from agar plates. The yeast species included 39 isolates of Aureobasidium pullulans, 17 of the genus Candida, 14 of the genus Rhodosporidium, 6 of the genus Cryptococcus, and 3 of the genus Rhodotorula. CONCLUSION: Yeast isolates associated with fleabane flowers included A. pullulans (39 isolates) and other yeast species (40 isolates). Such yeast isolates may have biotechnological potential.

Yeasts Associated with Roots of the Endemic Plant Mankyua chejuense

“…Yeast isolates from the tiger lily flower have been reported to comprise of Aureobasidium pullulans (97.9%, 95 isolates) (Kim and Kim, 2015a), whereas those associated with fleabane flowers include A. pullulans (49.3%, 39 isolates), Candida (21.5%, 17 isolates), Rhodosporidium (17.7%, 14 isolates), Cryptococcus (7.5%, six isolates), and Rhodotorula (3.7%, three isolates) (Kim and Kim 2015b). In addition, A. pullulans from flowers have also been found to produce several biosurfactants, depending on their phylogenetic class (Kim et al, 2015;Kim et al, in press).…”