Structural Characterization of Mannoglucan Isolated from Ophiocordyceps sobolifera and Its Antioxidant Activities

Abstract

A novel polysaccharide structure (PS-T80) was collected from Ophiocordyceps sobolifera biomass and characterized via a combination of chemical and spectral analyses. Employing high-performance gel permeation chromatography (HPGPC), the average molecular weight is proven to be 7.4 × 10 4 Da. Furthermore, a sugar composition analysis of the obtained polysaccharide suggests two main sugars, β- d -glucose and α- d -mannose, at a molar ratio of 2:1, respectively, in the backbone. The structure analysis unveils that PS-T80 is a mannoglucan, possessing the repeating unit of [→3)-β- d -Glcp-(1 → 3)-α- d -Manp-(1 → 3)-β- d -Glcp-(1→] n . Such a configuration could be considered a novel polysaccharide. Impressively, in vitro antioxidant tests revealed that PS-T80 has a promising antioxidant activity. These results demonstrate that the obtained PS is a potential bioactive material for biomedical applications.

Evaluation of the “Relative Ordered Structure of Hericium erinaceus Polysaccharide” from Different Origins: Based on Similarity and Dissimilarity

“…Representative examples that represent the regularity of the relative ordered structure of mushroom polysaccharides: (a) β-(1 → 3)-glucan derived from Dictyophora ruficans (DRP-20), (b) β-(1 → 6)-glucan derived from Agrocybe cylindracea (JACP-50p), (c) α-glucan derived from Hypsizygus marmoreus (SHMP-20), (d) mannoglucan derived from Ophiocordyceps sobolifera, (e) fucogalactoglucan derived from A. cylindracea (SACP-40), (f) galactoglucan derived from Hypsizygus marmoreus (SHMP-80), (g) mannogalactoglucan derived from Agaricus bisporus, (h) fucogalactan derived from A.…”

Rich d-Fructose-Containing Polysaccharide Isolated from Myxopyrum smilacifolium Roots toward a Superior Antioxidant Biomaterial

“…The ABTS radical scavenging activity of the presented polysaccharides is higher than that of both Ophiocordyceps sobolifera polysaccharides (IC 50 : 4.83 mg/mL). 46 Furthermore, the achieved IC 50 value, 3.69 mg/mL, considerably outperforms those of variously reported polysaccharides, as shown in Table S1 .…”

“…The DPPH radical scavenging activity of the PS-MSR is higher than those of both Cordyceps sinensis polysaccharides (IC 50 of 1.23 mg/mL) 47 and O. sobolifera polysaccharides (IC 50 of 0.97 mg/mL). 46 Moreover, the found DPPH radical scavenging activity of the PS-MSR is higher than those of both the Ganoderma lucidum polysaccharides. 48 The mentioned pieces of evidence suggest a great antioxidant capability of the PS-MSR sample toward antioxidant properties of rich d -fructose-containing polysaccharides, which has been explored from the roots of M. smilacifolium for the first time.…”

“…The DPPH radical scavenging activity of the PS-MSR is higher than those of both Cordyceps sinensis polysaccharides (IC 50 of 1.23 mg/mL) and O. sobolifera polysaccharides (IC 50 of 0.97 mg/mL) . Moreover, the found DPPH radical scavenging activity of the PS-MSR is higher than those of both the Ganoderma lucidum polysaccharides .…”

“…The ABTS radical scavenging activity of the presented polysaccharides is higher than that of both Ophiocordyceps sobolifera polysaccharides (IC 50 : 4.83 mg/ mL). 46 Furthermore, the achieved IC 50 value, 3.69 mg/mL, considerably outperforms those of variously reported polysaccharides, as shown in Table S1. The DPPH radical scavenging test is also conducted to study the activity of antioxidants quantifying and comparing the free radical scavenging capabilities of various antioxidants.…”