Structural characterization of a rhamnolipid-type biosurfactant produced by Pseudomonas aeruginosa MR01: Enhancement of di-rhamnolipid proportion using gamma irradiation

Lotfabad, Tayebe Bagheri; Abassi, Habib; Ahmadkhaniha, Reza; Roostaazad, Reza; Masoomi, Fatemeh; Zahiri, Hossein Shahbani; Ahmadian, Gholamreza; Vali, Hojatollah; Noghabi, Kambiz Akbari

doi:10.1016/j.colsurfb.2010.06.026

Cited by 98 publications

(71 citation statements)

References 37 publications

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“…competing with RL biosynthesis. It is well reported that growth on hydrocarbons and fatty acids increase RL production in P. aeruginosa (Nitschke et al 2005;Li et al 2011;Lotfabad et al 2010). Zhang et al (2012) showed that when P. aeruginosa was grown on octadecanoic acid, a combination of de novo fatty acid synthesis and β-oxidation contributed to the lipid precursor pool.…”

Section: Discussionmentioning

confidence: 98%

Development and validation of an ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method for the quantitative determination of rhamnolipid congeners

Rudden

Tsauosi

Marchant

et al. 2015

Appl Microbiol Biotechnol

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Rhamnolipids (RLs) are synthesised as a complex mixture of congeners comprising either one or two molecules of rhamnose glycosidically linked to a dimer of 3-hydroxy fatty acids varying in chain length and degree of saturation. Currently, HPLC-MS/MS is the most precise and accurate method for RL determination, while accurate quantification is limited. In this study, a rapid ultra pressure liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method was developed and validated for the rapid and quantification of individual RL congeners. Increased RLs specificity was achieved using tandem mass spectrometry in multiple reaction monitoring (MRM) mode which was used to quantify RL isomer pairs such as Rha-Rha-C8-C10/Rha-Rha-C10-C8 which are difficult to resolve chromatographically. UPLC showed an 18-fold reduction in retention time for Rha-Rha-C10-C10 (1.07 min) and a 17-fold reduction for Rha-C10-C10 (1.36), the major rhamnolipids present, compared to HPLC, with a total run time less than 2.2 min. The results show that the linear range for the main RL congeners (Rha-C10-C10 and Rha-Rha-C10-C10) is 0.1 to 100 μg/mL. The LOD and LOQ for Rha-C10-C10 is 0.05 and 0.1 μg/mL and for Rha-Rha-C10-C10 is 0.1 and 0.5 μg/mL, respectively. The method was validated for linearity, intra- and inter-day precision and accuracy in accordance with FDA guidelines. The method was applied for the quantification of 14 individual RL congeners produced by Pseudomonas aeruginosa ST5 and comparison of RLs composition on four different carbon sources. Quantification of the individual congeners showed a conserved congener distribution irrespective of carbon source with a preferential selection for C10 β-hydroxyacids as the lipid component of RLs. The only statistically significant differences detected were between actual RL yields on the various carbon sources.

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Section: Discussionmentioning

confidence: 98%

Development and validation of an ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method for the quantitative determination of rhamnolipid congeners

Rudden

Tsauosi

Marchant

et al. 2015

Appl Microbiol Biotechnol

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“…It has been reported that rhamnolipid production by P. aeruginosa is inefficient under oxygen-limiting conditions, and as the agitation rates increase (up to 150-250 rpm), rhamnolipid production also increases due to a higher dissolved oxygen concentration in the medium (Abbasi et al, 2012;Abdel-Mawgoud et al, 2009;Aparna et al, 2012;Bharali and Konwar, 2011;Cha et al, 2008;Haba et al, 2003;Lotfabad et al, 2009Lotfabad et al, , 2010Pacheco et al, 2012;Wei et al, 2005).…”

Section: Resultsmentioning

confidence: 98%

“…Other congeners frequently found include mono-and di-rhamnolipids with acyl chains containing 8, 10, 12 or 14 carbons, mostly saturated, and, less often, containing one or two double bonds, as well as with only one b-hydroxy fatty acid (Abbasi et al, 2012;Aparna et al, 2012;Benincasa and Accorsini, 2008;Benincasa et al, 2004;Bharali and Konwar, 2011;George and Jayachandran, 2013;Haba et al, 2003;Lotfabad et al, 2010;Pereira et al, 2012;Zhang et al, 2014). Exceptionally, acyl chains with a higher (C 18 , C 22 and C 24 ) or an odd number of carbons can be found (Hosková et al, 2013;Nie et al, 2010).…”

Section: Biosurfactants Chemical Characterizationmentioning

confidence: 95%

“…Different substrates have been used to produce rhamnolipids, including orange peel (George and Jayachandran, 2009), olive oil (Wei et al, 2005), coconut oil (George and Jayachandran, 2013), wastes from the sunflower-oil production process (Benincasa and Accorsini, 2008;Benincasa et al, 2004), waste frying oil (Haba et al, 2003), soybean oil (Abbasi et al, 2012;Cha et al, 2008;Lotfabad et al, 2010) or molasses (Aparna et al, 2012), with productivities between 3.5 and 12 g of biosurfactant per liter. However, it has to be taken into consideration that in some of these works, the biosurfactant concentration was calculated indirectly as rhamnose concentration (Benincasa and Accorsini, 2008;Benincasa et al, 2004;Jayachandran, 2009, 2013;Haba et al, 2003).…”

Section: Use Of Alternative Substrates For Biosurfactant Productionmentioning

confidence: 99%

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Bioconversion of agro-industrial by-products in rhamnolipids toward applications in enhanced oil recovery and bioremediation

Gudiña

Rodrigues

Alves

et al. 2015

Bioresource Technology

172

108

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In this work, biosurfactant production by a Pseudomonas aeruginosa strain was optimized using low-cost substrates. The highest biosurfactant production (3.2 g/l) was obtained using a culture medium containing corn steep liquor (10% (v/v)) and molasses (10% (w/v)). The biosurfactant reduced the surface tension of water up to 30 mN/m, and exhibited a high emulsifying activity (E24=60%), with a critical micelle concentration as low as 50 mg/l. The biosurfactant produced in this alternative medium was characterized as a mixture of eight different rhamnolipid congeners, being the most abundant the mono-rhamnolipid Rha-C10-C10. However, using LB medium, nine different rhamnolipid congeners were identified, being the most abundant the di-rhamnolipid Rha-Rha-C10-C10. The rhamnolipid mixture produced in the alternative medium exhibited a better performance in removing oil from contaminated sand when compared with two chemical surfactants, suggesting its potential use as an alternative to traditional chemical surfactants in enhanced oil recovery or bioremediation.

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“…By performing this technique, up to 17 different rhamnolipid congeners were recognized in MR01 biosurfactant mixture when grown on glucose in M1 medium using glucose as sole carbon source (43). P. aeruginosa MR01, grown in M1 medium, produced a mixture of rhamnolipids with capability of surface tension reduction to 27 mN/m.…”

Section: Discussionmentioning

confidence: 99%

Assessment of Antibacterial Capability of Rhamnolipids Produced by Two Indigenous Pseudomonas aeruginosa Strains

Lotfabad

Shahcheraghi

Shooraj

2012

Jundishapur J Microbiol

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This study shows that rhamnolipid mixtures of MR01 and MASH1 have antimicrobial efficacy against Gram positive bacteria and may be evaluated as antimicrobial agent against multidrugresistant clinical pathogenic isolates. Background:In recent decades, biological properties of biosurfactants, particularly glycolipids, including antimicrobial, antifungal, and anti-cellular effects have been projected in many research studies. The unique advantages of these compounds in terms of production and environment made them important as antimicrobial agents. Objectives: This study aims to evaluate probable antibacterial and antifungal properties of MR01 and MASH1 biosurfactants against several specified microorganisms. Materials and Methods:In this article antibacterial activities of two rhamnolipid mixtures of MR01 and MASH1 were studied. MR01 and MASH1 are glycolipid-type biosurfactants that are being produced by two strains of Pseudomonas aeruginosa MR01 and MASH1, respectively. Antibacterial effects of two biosurfactants were assessed by disc diffusion test method and determination of minimum inhibitory concentration (MIC). Results: They exhibit not only excellent surface activity but also remarkable inhibitory effect against Gram-positive bacteria. According to results, although none of two biosurfactants showed significant effects on Gram negative bacteria growth inhibition, both assessing methods confirmed the growth inhibition of Gram-positive bacteria by MR01 and MASH1 biosurfactants. Conclusions: According to results of this study, MR01 and MASH1 biosurfactants had antimicrobial efficacy against Gram positive bacterial groups. This effect is comparable to antibiotics and therefore the future use of these biosurfactants as broad spectrum antibiotics is highly promising. These features of biosurfactants (BS) should broaden its applications in new advanced technologies. Future studies will be performed on characterization and isolation of biologically active fraction of the rhamnolipid biosurfactant produced by P. aeruginosa strains. This bioactive compound may be evaluated as a potent antimicrobial agent to be applied against a panel of pathogenic micro-organisms including a few multidrug-resistant (MDR) clinical pathogenic isolates such as methicillin-resistant Staphylococcus aureus (MRSA) and other MDR pathogenic strains.

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Structural characterization of a rhamnolipid-type biosurfactant produced by Pseudomonas aeruginosa MR01: Enhancement of di-rhamnolipid proportion using gamma irradiation

Cited by 98 publications

References 37 publications

Development and validation of an ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method for the quantitative determination of rhamnolipid congeners

Development and validation of an ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method for the quantitative determination of rhamnolipid congeners

Bioconversion of agro-industrial by-products in rhamnolipids toward applications in enhanced oil recovery and bioremediation

Assessment of Antibacterial Capability of Rhamnolipids Produced by Two Indigenous Pseudomonas aeruginosa Strains

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