“…Different substrates have been used to produce rhamnolipids, including orange peel (George and Jayachandran, 2009), olive oil (Wei et al, 2005), coconut oil (George and Jayachandran, 2013), wastes from the sunflower-oil production process (Benincasa and Accorsini, 2008;Benincasa et al, 2004), waste frying oil (Haba et al, 2003), soybean oil (Abbasi et al, 2012;Cha et al, 2008;Lotfabad et al, 2010) or molasses (Aparna et al, 2012), with productivities between 3.5 and 12 g of biosurfactant per liter. However, it has to be taken into consideration that in some of these works, the biosurfactant concentration was calculated indirectly as rhamnose concentration (Benincasa and Accorsini, 2008;Benincasa et al, 2004;Jayachandran, 2009, 2013;Haba et al, 2003).…”