Structural characterization of a polysaccharide and a β-glucan isolated from the edible mushroom Flammulina velutipes

Purification and Characterization of a Novel Exo-β-1,3-1,6-glucanase from the Fruiting Body of the Edible Mushroom Enoki (Flammulina velutipes)

“…F. velutipes cell-wall -1,3-glucan is believed to have a repeating unit of laminaritetraose with a -1,6-branch. 13) Despite the presence of a -1,6-branch, FvBGL1 released glucose in a time-dependent manner without production of oligosaccharides (Fig. 3).…”

“…Besides S-and R-glucan, stagespecific synthesis of a -1,3-1,6-water soluble glucan (WSG) has been observed in Basidiomycete Schizophyllum commune during the development of their fruit bodies. 39,40) Furthermore, Smiderle et al 13) extracted xylomannan from F. velutipes fruiting bodies, indicating the complexity of F. velutipes cell-wall constituents.…”

“…Multiple enzyme systems are required for the metabolism of the Enoki cell-wall, which consists of complex polysaccharides of branched -1,3-glucan and xylomannan. 13) Specifically, -1,3-glucanases are of great importance, since the major structural component of the fruiting body cell wall is a -1,3-glucan with partial -1,6 intrachain linkages. To date, two types of -glucanase activity have been observed in fungi: one is an extracellularly secreted -glucanase and the other a cell-wall-associated -glucanase.…”

Effects of Dietary Flammulina velutipes Mycelium on Broiler Chick Performance, Pathogenic Bacterial Counts in Caecal Contents and Amount of NH3in Excreta

“…버섯은 그 자체로도 영양원이 풍부하지만 근래 들어서는 버 섯 배지에 기능성 소재나 특수 성분을 함유한 원료를 첨가하여 균 사체 및 자실체를 생산함으로써 그 성분이 버섯으로 이행되어 특수 성을 갖는 버섯을 인위적으로 생산하기에 이르렀으며, 버섯의 영양 가와 약용가치가 점차 밝혀짐에 따라 그 수요도 증가하고 있다. 이와 같이 버섯은 일반식품에 비하여 무 농약 천연식품으로서 다 른 농작물에서 합성이 적은 라이신 등 필수아미노산이나 무기질 등 이 비교적 다양하게 함유되어 있으며, 버섯 성분 중에는 단백질, 아미노산 등이 풍부할 뿐만 아니라 β-glucan, 비타민 및 미량원소 등이 함유되어 있어서 새로운 기능성 소재로 각광받고 있다 (Hui et al, 2002;Furlani and Godoy, 2007;Yang et al, 2002;Smiderle et al, 2006). 버섯의 생물학적 활성에 관하여 국내외의 많은 보고가 있으며 (Yoshioka et al, 1985;Kim et al, 1992;Kim et al, 1993), 한국 버섯류의 항종양 활성에 관한 연구뿐만 아니라 (Chai, 2001;Kim et al, 1980;Chang and Miles, 1989), 항균활성물질에 관 한 연구들도 많이 보고되고 있다 (Midland et al, 1982;Kupka et al, 1979;Okamoto et al, 1993).…”

Antifreeze Activity of Xylomannan from the Mycelium and Fruit Body of <i>Flammulina velutipes</i>

“…The resultant mycelium was freeze-dried. After milling these dried samples from the mycelium and fruit body, the isolation of xylomannan was done by the modified Smiderle method Smiderle et al, 2006 as shown in Fig.1. The milled dried samples were submitted to aqueous x3 at 100 , 1,000 mL and alkaline extractions.…”