Purification and Characterization of a Novel Exo-β-1,3-1,6-glucanase from the Fruiting Body of the Edible Mushroom Enoki (<i>Flammulina velutipes</i>)

Fukuda, Kenji; Hiraga, Michika; Asakuma, Shinichiro; Arai, Ikichi; Sekikawa, Mitsuo; Urashima, Tadasu

doi:10.1271/bbb.80213

Cited by 22 publications

(9 citation statements)

References 39 publications

(45 reference statements)

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“…A large suite of β-glucanases, chitinases, laccases, endo-β-1,4-mannanases, α-1,3-mannosidases were developmentally regulated, many of which are also expanded in Agaricomycetes (Table 1, Supplementary Fig 7). The expression of glucan-, chitin-and mannose-active enzymes is consistent with active FCW remodeling during fruiting body formation and recent reports of similar genes upregulated in the fruiting bodies of Lentinula 12,13,26 , Flammulina 27 and Coprinopsis 28 . Kre9/Knh1 homologs are developmentally regulated in all species and are overrepresented in mushroom-forming fungi (P = 1.45×10 −5 , FET).…”

Section: Resultssupporting

confidence: 87%

Transcriptomic atlas of mushroom development highlights an independent origin of complex multicellularity

Krizsán

Almási

Merényi

et al. 2018

Preprint

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We constructed a reference atlas of mushroom formation based on developmental transcriptome data of six species and comparisons of >200 whole genomes, to elucidate the core genetic program of complex multicellularity and fruiting body development in mushroom-forming fungi (Agaricomycetes). Nearly 300 conserved gene families and >70 functional groups contained developmentally regulated genes from five to six species, covering functions related to fungal cell wall (FCW) remodeling, targeted protein degradation, signal transduction, adhesion and small secreted proteins (including effector-like orphan genes). Several of these families, including F-box proteins, protein kinases and cadherin-like proteins, showed massive expansions in Agaricomycetes, with many convergently expanded in multicellular plants and/or animals too, reflecting broad genetic convergence among independently evolved complex multicellular lineages. This study provides a novel entry point to studying mushroom development and complex multicellularity in one of the largest clades of complex eukaryotic organisms.

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Section: Resultssupporting

confidence: 87%

Transcriptomic atlas of mushroom development highlights an independent origin of complex multicellularity

Krizsán

Almási

Merényi

et al. 2018

Preprint

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“…Fungi such as ascomycetes and basidiomycetes produce enzymes associated with these polysaccharides. Some of these fungal glycoside hydrolases (GH) act on cell wall components and are responsible for morphological changes (Adams, 2004;Fukuda et al, 2008;Mahadevan and Mahadkar, 1970;Wessels and Niederpruem, 1967).…”

Section: 1-glucanasesmentioning

confidence: 99%

Senescence of the Lentinula edodes Fruiting Body After Harvesting

Sakamoto¹,

Nakade²,

Konno³

et al. 2012

Food Quality

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“…It results from the concerted expression of structural and regulatory genes (Martin et al, 2008;Stajich et al, 2010;Ohm et al, 2011;Muraguchi et al, 2015;Nagy et al, 2016;Lau et al, 2018) as well as other processes, such as alternative splicing Krizs an et al, 2019), allele-specific gene expression and probably selective protein modification (Pelkmans et al, 2017;Krizs an et al, 2019). Known structural genes include ones coding for hydrophobins (Lugones et al, 1996;W€ osten et al, 1999;Bayry et al, 2012), lectins (Cooper et al, 1997;Boulianne et al, 2000;Hassan et al, 2015) and several cell wall chitin and glucan-active CAZymes (Wessels, 1994;Fukuda et al, 2008;Sakamoto et al, 2011), and probably include genes for ceratoplatanins, which are expansin-like proteins (Sipos et al, 2017;Krizs an et al, 2019), among others. Regulators of fruiting body development have been characterized in several species, in particular in Coprinopsis cinerea Cheng et al, 2013;de Sena-Tomas et al, 2013;Muraguchi et al, 2015;Masuda et al, 2016) and S. commune (Ohm et al, 2010(Ohm et al, , 2011Pelkmans et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Comparative genomics reveals unique wood‐decay strategies and fruiting body development in the Schizophyllaceae

et al. 2019

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Summary Agaricomycetes are fruiting body‐forming fungi that produce some of the most efficient enzyme systems to degrade wood. Despite decades‐long interest in their biology, the evolution and functional diversity of both wood‐decay and fruiting body formation are incompletely known. We performed comparative genomic and transcriptomic analyses of wood‐decay and fruiting body development in Auriculariopsis ampla and Schizophyllum commune (Schizophyllaceae), species with secondarily simplified morphologies, an enigmatic wood‐decay strategy and weak pathogenicity to woody plants. The plant cell wall‐degrading enzyme repertoires of Schizophyllaceae are transitional between those of white rot species and less efficient wood‐degraders such as brown rot or mycorrhizal fungi. Rich repertoires of suberinase and tannase genes were found in both species, with tannases restricted to Agaricomycetes that preferentially colonize bark‐covered wood, suggesting potential complementation of their weaker wood‐decaying abilities and adaptations to wood colonization through the bark. Fruiting body transcriptomes revealed a high rate of divergence in developmental gene expression, but also several genes with conserved expression patterns, including novel transcription factors and small‐secreted proteins, some of the latter which might represent fruiting body effectors. Taken together, our analyses highlighted novel aspects of wood‐decay and fruiting body development in an important family of mushroom‐forming fungi.

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Purification and Characterization of a Novel Exo-β-1,3-1,6-glucanase from the Fruiting Body of the Edible Mushroom Enoki (Flammulina velutipes)

Cited by 22 publications

References 39 publications

Transcriptomic atlas of mushroom development highlights an independent origin of complex multicellularity

Transcriptomic atlas of mushroom development highlights an independent origin of complex multicellularity

Senescence of the Lentinula edodes Fruiting Body After Harvesting

Comparative genomics reveals unique wood‐decay strategies and fruiting body development in the Schizophyllaceae

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