Structural characterization, antioxidant and cytotoxic effects of iron nanoparticles synthesized using Asphodelus aestivus Brot. aqueous extract

Abstract

<jats:title>Abstract</jats:title><jats:p><jats:italic>ASP</jats:italic> was used to synthesize FeNP<jats:sub>A</jats:sub>. They were characterized by UV-vis spectroscopy, FT-IR, TEM, SEM, XRD and ZP. The aim of this study was to evaluate in vitro cytotoxic activity and antioxidant acitivities of FeNP<jats:sub>A</jats:sub> and <jats:italic>ASP</jats:italic>. The antioxidant properties were evaluated using DPPH, ABTS<jats:sup>+</jats:sup> and H<jats:sub>2</jats:sub>O<jats:sub>2</jats:sub> assays. FeNP<jats:sub>A</jats:sub> had higher antioxidant activity comparing to <jats:italic>ASP</jats:italic> according to DPPH (IC<jats:sub>50</jats:sub>: 3.48 μg/mL) and ABTS<jats:sup>+</jats:sup> (60.52%) assays. Anti-cancer activities of FeNP<jats:sub>A</jats:sub> and <jats:italic>ASP</jats:italic> were investigated in breast cancer, melanoma and control cell lines. FeNP<jats:sub>A</jats:sub> was more cytotoxic than <jats:italic>ASP</jats:italic> in MCF-7, MeWo, CHL-1, and HEL 299 cells. FeNP<jats:sub>A</jats:sub> had shown that mitochondria induce apoptosis through stress in MDA-MB-231, and cells MeWo. <jats:italic>ASP</jats:italic> also induced apoptosis 2.23-fold in MCF-7 cells. Progesterone receptor gene expression showed a 10-fold increase in a hormone-dependent MCF-7 cell line in <jats:italic>ASP</jats:italic>, and FeNP<jats:sub>A</jats:sub> treatment. Expressions of BCL6, CXCL12, DNAJC15, RB1 and TPM1 in melanoma cancer cell lines were significantly increased in <jats:italic>ASP</jats:italic> and FeNP<jats:sub>A</jats:sub> administration. It had been shown that FeNP<jats:sub>A</jats:sub> regulates gene expressions that may be considered important in terms of prognosis in breast cancer and melanoma cell lines and it is suggested that gene expressions regulated by FeNP<jats:sub>A</jats:sub> are also evaluated in animal models in vivo.</jats:p>

Cytotoxic effects of silver nanoparticles synthesized using Asphodelus aestivus Brot. aqueous extract

“…Cell lines were cultured and maintained in mediums (EMEM for MCF-7, MeWo, HEL-299, WI-38 cell lines, DMEM for CHL-1 cell line and L15 for MDA-MB-231 cell line) containing 1% L-glutamine supplemented with 10% inactivated FBS and 1% penicillin/streptomycin and were maintained in a humidified incubator at 37°C with 5% CO 2 . Analysis of cell viability was carried with trypan blue test [33].…”

“…The cells were incubated after the addition of AnnexinV-EGFP and propidium iodide (PI). AnnexinV-EGFP and propidium iodide (PI) [33].…”

Phytoantioxidant Functionalized Nanoparticles: A Green Approach to Combat Nanoparticle-Induced Oxidative Stress

“…Similarly, iron NPs produced using Asphodelus aestivus aerial parts scavenged DPPH with IC 50 3.48 μ g/mL [ 182 ]. In addition, Rajput et al [ 183 ] evaluated the antioxidant potential of Ag NPs ( Atropa acuminata leaf mediated) using DPPH, H 2 O 2 , and O 2 ·− assay with IC 50 16.08, 25.4, and 21.12 μ g/mL, which is lower than standard ascorbic and gallic acid (IC 50 27.68-30.48 μ g/mL).…”

“…The thermal stability of NPs was evaluated using thermal gravimetric analysis (TGA) in a single study ( Figure 4 and Table 1 ). Furthermore, elemental analysis was carried out using energy-dispersive X-ray analysis (EDX), which is used to detect impurities [ 67 , 163 , 166 , 169 , 177 , 182 , 183 ].…”