Structural characterization and α-glycosidase inhibitory activity of a novel polysaccharide fraction from Aconitum coreanum

Song, Jianxi; Wu, Ye; Ma, Xiaojing; Feng, Lijuan; Wang, Zhiguo; Jiang, Guiquan; Tong, Haibin

doi:10.1016/j.carbpol.2019.115586

Cited by 43 publications

(15 citation statements)

References 38 publications

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“…Briefly, samples (2 mg) were hydrolyzed with anhydrous methanol solution (0.5 ml) containing 1 M hydrochloric acid under nitrogen atmosphere at 80°C for 16 h. The hydrolysate was further hydrolyzed with 2 M TFA (0.5 ml) for 1 h. Ethanol was used to eliminate excess acid. The resulting product was derived with PMP reagent via a previously described method ( Song et al, 2019 ). Excessive PMP reagent was removed through chloroform extraction.…”

Section: Methodsmentioning

confidence: 99%

Characterisation, Chain Conformation and Antifatigue Effect of Steamed Ginseng Polysaccharides With Different Molecular Weight

Jiao

Liu

et al. 2021

Front. Pharmacol.

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Two polysaccharides were obtained from steamed ginseng via ultrafiltration, and their physical–chemical properties, solution properties and antifatigue activities were studied. WSGP-S3 and WSGP-G3 were acid heteropolysaccharides with the molecular weights of 2.03 × 104 and 4.86 × 104, respectively. They were composed of different molar ratios of the monosaccharides Rha, GlcA, GalA, Glc, Gal, and Ara. The results of size-exclusion chromatography–multiangle laser light scattering analysis, Conge red staining and Circular dichroism spectroscopy revealed that WSGP-S3 exhibited a random conformation of branched clusters in solution. By contrast, WSGP-G3 exhibited an ordered conformation, including helix-like conformations in aqueous solution. Antifatigue activity tests proved that WSGP-S3 markedly prolonged the exhaustive swimming time of fatigued mice; increased liver and muscle glycogen levels and superoxide dismutase, catalase, glutathione peroxidase activities and decreased blood lactic acid, nitrogen and malondialdehyde levels compared with the control treatment. Moreover, it enhanced spleen cell proliferation in fatigued mice. By contrast, WSGP-G3 had no significant effect on fatigued mice. The results showed that WSGP-S3 might have a major contribution to the antifatigue effects of steamed ginseng polysaccharides and could be a potential anti-fatigue polysaccharide.

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Section: Methodsmentioning

confidence: 99%

Characterisation, Chain Conformation and Antifatigue Effect of Steamed Ginseng Polysaccharides With Different Molecular Weight

Jiao

Liu

et al. 2021

Front. Pharmacol.

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“…3, δ71.2, δ77.7, δ79.6, δ76.3, and δ61.7, and the carbon and hydrogen spectrum signal should be for 1,4-glc analyzed through the HSQC spectrum. And some previous studies also have similar result (Liu et al, 2017;Zhang et al, 2019;Song et al, 2019) All the glycosidic bonds were assigned to the NMR signal by the same method.…”

Section: Nmr Spectroscopy Analysis Of Scp-80-imentioning

confidence: 62%

Structural characterization of a non‐starch polysaccharide from sweet corn cobs

Wang

Xiu

Han

et al. 2022

Food Processing Preservation

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In order to improve the utilization value of sweet corn cobs, we extracted a new nonstarch polysaccharide from sweet corn cobs and named it SCP-80-I. It showed that SCP-80-I was a neutral homogeneous polysaccharide composed of glucose with a molecular weight of 175.453 kDa. We also explore the structural features of SCP-80-I and we found that it possessed a backbone of →4-Glc-1 → 4-Glc-1 → 4,6-Glc-1 → 4-Glc-1→, and the branched chain was →6-Glc-1 → linked to the main chain through O-6 of →4,6-Glc-1→. We predicted that SCP-80-I may have a function of antioxidation and hypoglycemic due to its structure. This study provides some reference value for sweet corn cob polysaccharides in the creation of functional food. Novelty impact statement:1. A new non-starch polysaccharide from sweet corn cobs (SCP-80-I) was obtained.2. SCP-80-I was composed of glucose with a molecular weight of 175.453 kDa.

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“…If the molecular weight of polysaccharides was to be determined accurately, they were usually puried by anion exchange chromatography and further separated by gel column chromatography. 17,26 But with the help of HPGPC, we could know whether the molecular weight of PTXPs extracted by different extraction methods was different. HPGPC, a type of size exclusion chromatography that separates analytes on the basis of molecular size, is suitable for the analysis of the molecular weight of PTXPs.…”

Section: Molecular Weight Of Puried Ptxpsmentioning

confidence: 99%

Study on extraction methods of polysaccharides from a processed product of Aconitum carmichaeli Debx.

Qiu

Long

et al. 2021

RSC Adv.

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Structural characterization and α-glycosidase inhibitory activity of a novel polysaccharide fraction from Aconitum coreanum

Cited by 43 publications

References 38 publications

Characterisation, Chain Conformation and Antifatigue Effect of Steamed Ginseng Polysaccharides With Different Molecular Weight

Characterisation, Chain Conformation and Antifatigue Effect of Steamed Ginseng Polysaccharides With Different Molecular Weight

Structural characterization of a non‐starch polysaccharide from sweet corn cobs

Study on extraction methods of polysaccharides from a processed product of Aconitum carmichaeli Debx.

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