Study on extraction methods of polysaccharides from a processed product of <i>Aconitum carmichaeli</i> Debx.

Qiu, Kuncheng; Li, Zunjiang; Long, Yingxin; Lu, Zhongyu; Zhu, Wei

doi:10.1039/d1ra03628a

Cited by 6 publications

(1 citation statement)

References 32 publications

(40 reference statements)

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“…The absorbance of dopaquinone, catalytically produced via L-DOPA (2.5 mM), was measured using a Thermo Arioskan Flash automatic microplate instrument (Thermo Corporation, Waltham, MA, USA) [24]. Tyrosinase (40 U/mL, PBS50 Mm) was added to the 96-well microtiter plate from left to right via a pipette and incubated at 37 • C for 10 min; following which, the substrate, L-DOPA, was added.…”

Section: Anti-aging Activity Assay 2111 Tyrosinase Inhibition Testmentioning

confidence: 99%

Extraction Optimization, UHPLC-Triple-TOF-MS/MS Analysis and Antioxidant Activity of Ceramides from Sea Red Rice Bran

Wang

Jia

Song

et al. 2022

Foods

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As a new type of salt-tolerant rice, sea red rice contains more minerals, proteins, and lipid compounds, and, in particular, its by-product rice bran may be used to replace other commercial rice brans as the main source of ceramides (Cers). However, the extraction rate of Cers is generally low, and it is crucial to seek an efficient extraction method. This study optimized the ultrasonic-assisted extraction of Cers from sea red rice bran using response surface methodology (RSM) and obtained a Cers yield of 12.54% under optimal conditions involving an extraction temperature of 46 °C, an extraction time of 46 min, and a material–to-liquid ratio of 5 g/mL. The Cers content in sea red rice bran was preliminarily analyzed using thin-layer chromatography, and the Cers content was determined via UHPLC-Triple-TOF-MS/MS after purification and separation using silica column chromatography. Forty-six different types of Cers were identified in sea red rice bran, of which Cer 18:0/24:0 (2OH), Cer 18:0/26:0, Cer 18:0/26:0 (2OH), and Cer 18:0/24:0 accounted for 23.66%, 17.54%, 14.91%, and 11.96%. Most of the Cers structures were mainly composed of sphingadienine. A biological activity assay indicated that Cers extracted from sea red rice bran had significant antioxidant and anti-aging properties. These findings indicate that the extracted Cers show great potential for applications in the cosmetic and pharmaceutical industries.

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Section: Anti-aging Activity Assay 2111 Tyrosinase Inhibition Testmentioning

confidence: 99%

Extraction Optimization, UHPLC-Triple-TOF-MS/MS Analysis and Antioxidant Activity of Ceramides from Sea Red Rice Bran

Wang

Jia

Song

et al. 2022

Foods

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Structural characterization of two Hericium erinaceus polysaccharides and their protective effects on the alcohol-induced gastric mucosal injury

et al. 2022

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Paotianxiong polysaccharides potential prebiotics: Structural analysis and prebiotic properties

Fu,

Tian,

Yang

et al. 2024

Food Chemistry

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Study on extraction methods of polysaccharides from a processed product of Aconitum carmichaeli Debx.

Abstract: By comparing the effects of extraction methods on the activity and properties of polysaccharide, a polysaccharide extraction process was screened and optimized.

Cited by 6 publications

References 32 publications

Extraction Optimization, UHPLC-Triple-TOF-MS/MS Analysis and Antioxidant Activity of Ceramides from Sea Red Rice Bran

Extraction Optimization, UHPLC-Triple-TOF-MS/MS Analysis and Antioxidant Activity of Ceramides from Sea Red Rice Bran

Structural characterization of two Hericium erinaceus polysaccharides and their protective effects on the alcohol-induced gastric mucosal injury

Paotianxiong polysaccharides potential prebiotics: Structural analysis and prebiotic properties

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