Structural characterization and hypoglycemic activity of an intracellular polysaccharide from Sanghuangporus sanghuang mycelia

Cheng, Junwen; Song, Jiling; Wei, Hang; Wang, Yanbin; Huang, Xubo; Liu, Yu; Lu, Na; He, Li; Lv, Guoying; Ding, Hongmei; Yang, Saozong; Zhang, Zuofa

doi:10.1016/j.ijbiomac.2020.08.202

Cited by 55 publications

(43 citation statements)

References 25 publications

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“…A typical characteristic of type 2 diabetes mellitus is IR. IR causes damage to peripheral cells that metabolise blood glucose, thereby leading to increased blood glucose levels (Cheng et al ., 2020). The changes in glucose consumption in the insulin‐resistant HepG2 cell model under the influence of different concentrations of SGP, SGP‐S, SGP‐G, and SGP‐I are shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

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Effects of simulated saliva‐gastrointestinal digestion on the physicochemical properties and bioactivities of Siraitia grosvenorii polysaccharides

Guo

Chen

Gong

et al. 2022

Int J of Food Sci Tech

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Summary The aim of this study was to investigate the effect of simulated saliva‐gastric digestion (SGID) on the physicochemical properties and bioactivity of Siraitia grosvenorii polysaccharides (SGPs). The results revealed that the digestibility (bioavailability) of SGPs was approximately 16.81%, 43.86%, and 52.38% after saliva digestion (SD), saliva‐gastric digestion (SGD), and SGID, respectively. SGID significantly altered the physicochemical properties of SGPs, such as total polysaccharide content (from 88.25% to 69.26%) and molecular weight (from 23.00 to 7.82). The structure of SGPs was significantly altered after digestion. In addition, SGID affected the biological activity of SGPs. after SGID, SGPs still had strong antioxidant activity, as well as α‐glucosidase, α‐amylase inhibition, and cellular hypoglycemic activity. However, the ability to bind bile acids and cholesterol in vitro was not affected. In conclusion, these results could provide a theoretical basis for investigating the mechanism of action of SGPs during digestion in vivo.

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Section: Resultsmentioning

confidence: 99%

“…The in vitro α ‐amylase inhibitory activity was determined according to the method of Cheng et al . (2020). Acarbose was used as a positive control.…”

Section: Methodsmentioning

confidence: 99%

Effects of simulated saliva‐gastrointestinal digestion on the physicochemical properties and bioactivities of Siraitia grosvenorii polysaccharides

Guo

Chen

Gong

et al. 2022

Int J of Food Sci Tech

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“…The heteronuclear single-quantum coherence (HSQC) correlation map from anomeric region showed C1/H1 signals at δ 5.38/99.70 ppm ( A ), δ 4.45/101.61 ppm ( B ), δ 4.71/103.10 ppm ( C ), δ 5.22/108.35 ppm ( D ), δ 5.20/109.20 ppm ( E ) and δ 5.26/97.60 ppm ( F ) ( Figure 5 C). The α-configurations of residues A , D , E and F were confirmed from the chemical shifts of anomeric protons of greater than δ 4.90 ppm, whereas the β-configurations of residues B and C were confirmed by the low frequency of H-1 and high frequency of C-1 signals [ 39 ]. The chemical shifts of H-1/H-2, H-2/H-3 and H-3/H-4 were observed at δ 5.38/3.62 ppm, δ 3.62/3.95 ppm and δ 3.95/3.64 ppm in 1 H– 1 H correlated spectroscopy ( 1 H- 1 H COSY) spectrum, thus the chemical shifts of H-1 to H-4 in residue A were assigned to be δ 5.38 ppm, δ 3.62 ppm, δ 3.95 ppm and δ 3.64 ppm, respectively.…”

Section: Resultsmentioning

confidence: 99%

Purification, Structural Characterization and Immunomodulatory Effects of Polysaccharides from Amomumvillosum Lour. on RAW 264.7 Macrophages

et al. 2021

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Amomum Villosum Lour. (A. villosum) is a folk medicine that has been used for more than 1300 years. However, study of the polysaccharides of A. villosum is seriously neglected. The objectives of this study are to explore the structural characteristics of polysaccharides from A. villosum (AVPs) and their effects on immune cells. In this study, the acidic polysaccharides (AVPG-1 and AVPG-2) were isolated from AVPs and purified via anion exchange and gel filtration chromatography. The structural characteristics of the polysaccharides were characterized by methylation, HPSEC-MALLS-RID, HPLC, FT-IR, SEM, GC-MS and NMR techniques. AVPG-1 with a molecular weight of 514 kDa had the backbone of → 4)-α-d-Glcp-(1 → 3,4)-β-d-Glcp-(1 → 4)-α-d-Glcp-(1 →. AVPG-2 with a higher molecular weight (14800 kDa) comprised a backbone of → 4)-α-d-Glcp-(1 → 3,6)-β-d-Galp-(1 → 4)-α-d-Glcp-(1 →. RAW 264.7 cells were used to investigate the potential effect of AVPG-1 and AVPG-2 on macrophages, and lipopolysaccharide (LPS) was used as a positive control. The results from bioassays showed that AVPG-2 exhibited stronger immunomodulatory activity than AVPG-1. AVPG-2 significantly induced nitric oxide (NO) production as well as the release of interleukin (IL)-6 and tumor necrosis factor alpha (TNF-α), and upregulated phagocytic capacities of RAW 264.7 cells. Real-time PCR analysis revealed that AVPG-2 was able to turn the polarization of macrophages to the M1 direction. These results suggested that AVPs could be explored as potential immunomodulatory agents of the functional foods or complementary medicine.

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“…Sanghuang contains several pharmacologically active compounds, including polysaccharides, flavonoids, pyrone, polyphenols, terpenoids, and sterols, which have been reported to cure diseases [6][7][8][9]. To date, the main polyphenols reported in Sanghuang include phellibaumin A, phelligridin C, phelligridin D, hypholomin B, protocatechualdehyde, interfungin B, and chlorogenic acid [10][11][12][13][14].…”

Section: Introductionmentioning

confidence: 99%

Optimization of Extraction Process and the Antioxidant Activity of Phenolics from Sanghuangporus baumii

et al. 2021

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Sanghuangporus baumii, is a widely used medicinal fungus. The polyphenols extracted from this fungus exert antioxidant, anti-inflammatory, and hypoglycemic effects. In this study, polyphenols from the fruiting bodies of S. baumii were obtained using the deep eutectic solvent (DES) extraction method. The factors affecting the extraction yield were investigated at different conditions. Based on the results from single-factor experiments, response surface methodology was used to optimize the extraction conditions. The scavenging ability of the polyphenols on •OH, DPPH, and ABTS+ was determined. The results showed that the DES system composed of choline chloride and malic acid had the best extraction yield (6.37 mg/g). The optimal extraction parameters for response surface methodology were as follows: 42 min, 58 ℃, 1:34 solid–liquid (mg/mL), and water content of 39%. Under these conditions, the yield of polyphenols was the highest (12.58 mg/g). At 0.30 mg/mL, the scavenging ability of the polyphenols on •OH, DPPH, and ABTS+ was 95.71%, 91.08%, and 85.52%, respectively. Thus, the method using DES was more effective than the conventional method of extracting phenolic compounds from the fruiting bodies of S. baumii. Moreover, the extracted polyphenols exhibited potent antioxidant activity.

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Structural characterization and hypoglycemic activity of an intracellular polysaccharide from Sanghuangporus sanghuang mycelia

Cited by 55 publications

References 25 publications

Effects of simulated saliva‐gastrointestinal digestion on the physicochemical properties and bioactivities of Siraitia grosvenorii polysaccharides

Effects of simulated saliva‐gastrointestinal digestion on the physicochemical properties and bioactivities of Siraitia grosvenorii polysaccharides

Purification, Structural Characterization and Immunomodulatory Effects of Polysaccharides from Amomumvillosum Lour. on RAW 264.7 Macrophages

Optimization of Extraction Process and the Antioxidant Activity of Phenolics from Sanghuangporus baumii

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