Structural Characteristics, Rheological Properties, and Antioxidant Activity of Novel Polysaccharides from “Deer Tripe Mushroom”

Abstract

The polysaccharide was extracted by water extraction and alcohol precipitation method from “deer tripe mushroom,” which was domesticated and bred from wild Auricularia delicata in Zambia. The structure of the deer tripe mushroom polysaccharide (DTMP) was characterized by gas chromatography-mass spectrometry (GC-MS), Fourier transform infrared spectroscopy (FT-IR), nuclear magnetic resonance spectroscopy (NMR), scanning electron microscope (SEM), and other analytical techniques. And the rheological and gel properties and antioxidant capacities of the polysaccharide were studied. The results showed that DTMP was mainly composed of mannose, fructose, glucose, and galacturonic acid, of which the galacturonic acid was the main component. By calculation, the molar ratio of mannose : fructose : glucose : galactoacid in DTMP is 0.8 : 14.8 : 1.0 : 26.32. The polysaccharide was mainly composed by α-1,4-glycosidic bond. DTMP solution showed a shear-thinning (pseudoplastic) behaviour, and its pseudoplasticity was more obvious at a concentration of 2%. The power law model was used to evaluate the viscosity curves of DTMP, and its viscosity and consistency indices both increased as the concentration increased, whereas both indices decreased as the concentration decreased. The viscosity of the polysaccharide solution changed as the pH changed: the polysaccharide solutions had a higher viscosity at pH = 10. DTMP showed gel-like behaviour (G′ ＞ G″), and the gel strength enhanced with the increase of concentration (2%–10%). In addition, the antioxidant experiment of DTMP showed that it had good antioxidant activity, and there was a significant dose-effect relationship between its activity and concentration in the low concentration range.

Two Novel Polysaccharides From Clitocybe squamulosa: Their Isolation, Structures, and Bioactivities

“…When the concentrations of different sample solutions were 0.1, 0.3, and 0.5%, respectively, G ’ and G ” values of CSFP suggested no cross-linking, while cross-formation occurred and the gel formation and elastic properties of CSFP1-β and CSFP2-α increased with increasing concentrations. Hu et al ( 61 ) extracted polysaccharide (DTMP) from “deer tripe mushroom” and studied its rheological and gel properties. The results indicated that the DTMP solution showed shear-thinning behavior (pseudoplasticity), and its pseudoplasticity was more obvious at a concentration of 2%.…”

Exploring the Phytochemical Compositions, Antioxidant Activity, and Nutritional Potentials of Edible and Medicinal Mushrooms

“…Moreover, it could potentially be effective in the role of preventive agents in combatting the pathogenesis of certain diseases [ 117 ]. Furthermore, it has been demonstrated that polysaccharides derived from deer tripe mushrooms ( A. delicata ) possess radical scavenging action [ 118 ]. The results from the DPPH and ABTS assays were relatively good but not comparable to vitamin C [ 118 ].…”

“…Furthermore, it has been demonstrated that polysaccharides derived from deer tripe mushrooms ( A. delicata ) possess radical scavenging action [ 118 ]. The results from the DPPH and ABTS assays were relatively good but not comparable to vitamin C [ 118 ]. The study found that the crude polysaccharide has strong antioxidant properties, with a clear dose-effect relationship at lower concentrations [ 118 ].…”

“…The results from the DPPH and ABTS assays were relatively good but not comparable to vitamin C [ 118 ]. The study found that the crude polysaccharide has strong antioxidant properties, with a clear dose-effect relationship at lower concentrations [ 118 ]. The antioxidant activity of C. indica was assessed using various techniques such as radical scavenging and reduction ability [ 119 ].…”

Anti-Obesity Effect of Auricularia delicate Involves Intestinal-Microbiota-Mediated Oxidative Stress Regulation in High-Fat-Diet-Fed Mice

“…Recent research has found that ADe extracts showed antimicrobial, hepatoprotective, and antioxidant effects [ 25 ]. Polysaccharides from ADe effectively scavenged -OH and O 2 - in vitro, indicating its antioxidant properties [ 27 ]. However, few studies have examined the regulatory effects of ADe on obesity and elucidated its underlying mechanisms [ 25 ].…”