Abstract:Auricularia delicate (ADe), an edible fungus belonging to the family Auriculariaceae and order Auriculariales, possesses antimicrobial, hepatoprotective, and antioxidant effects. In this study, after systematic analysis of its composition, ADe was administered to high-fat-diet (HFD)-fed mice to investigate its anti-obesity effect. ADe significantly controlled body weight; alleviated hepatic steatosis and adipocyte hypertrophy; reduced aspartate aminotransferase, total cholesterol, insulin, and resistin; and in… Show more
“…On the other hand, some authors attributed metabolic deregulations and oxidative stress to changes in the intestinal microbiota composition caused by high-fat or high-fructose diets. Thus, ameliorative effects could be related to the prebiotic character of some polyphenols [ 42 , 43 , 44 ]. In fact, in a previous study, chia defatted flour showed positive effects on the intestinal microbiota using an in vitro gastrointestinal digestion model (increasing Lactobacillus and decreasing Enterobacteriaceae ) [ 11 ].…”
Today, consumption of diets rich in saturated fat and fructose, associated with a variety of metabolic deregulations, has increased. The aim of this study was to evaluate the effect of dietary supplementation with a residue of defatted chia seed on a diet with low nutritional quality. To do this, C57BL/6 male mice were fed with the Control (C), Low-Nutritional-Quality (LNQ), or supplemented-with-chia-defatted-flour (LNQ+C) diets. After 12 weeks, the glucose and lactate levels were determined in the serum, liver, and kidney, along with reactive oxygen species (ROS) levels, antioxidant enzyme activity, reduced glutathione (GSH), and protein oxidation (AOPP). The LNQ diet increased the glucose and lactate levels (+25% and +50% approx. in the liver, with respect to the control group) and generated oxidative stress by modifying the levels of ROS and the activity of antioxidant enzymes, causing oxidative damage to proteins (+12% in the liver, with respect to the control). Chia supplementation helped to restore the glucose to control levels and modulate the endogenous antioxidant system, resulting in a decrease in protein oxidation products with no differences compared to the control group. In conclusion, supplementation with chia showed beneficial effects on the general health of mice, even when fed a low-nutritional-quality diet.
“…On the other hand, some authors attributed metabolic deregulations and oxidative stress to changes in the intestinal microbiota composition caused by high-fat or high-fructose diets. Thus, ameliorative effects could be related to the prebiotic character of some polyphenols [ 42 , 43 , 44 ]. In fact, in a previous study, chia defatted flour showed positive effects on the intestinal microbiota using an in vitro gastrointestinal digestion model (increasing Lactobacillus and decreasing Enterobacteriaceae ) [ 11 ].…”
Today, consumption of diets rich in saturated fat and fructose, associated with a variety of metabolic deregulations, has increased. The aim of this study was to evaluate the effect of dietary supplementation with a residue of defatted chia seed on a diet with low nutritional quality. To do this, C57BL/6 male mice were fed with the Control (C), Low-Nutritional-Quality (LNQ), or supplemented-with-chia-defatted-flour (LNQ+C) diets. After 12 weeks, the glucose and lactate levels were determined in the serum, liver, and kidney, along with reactive oxygen species (ROS) levels, antioxidant enzyme activity, reduced glutathione (GSH), and protein oxidation (AOPP). The LNQ diet increased the glucose and lactate levels (+25% and +50% approx. in the liver, with respect to the control group) and generated oxidative stress by modifying the levels of ROS and the activity of antioxidant enzymes, causing oxidative damage to proteins (+12% in the liver, with respect to the control). Chia supplementation helped to restore the glucose to control levels and modulate the endogenous antioxidant system, resulting in a decrease in protein oxidation products with no differences compared to the control group. In conclusion, supplementation with chia showed beneficial effects on the general health of mice, even when fed a low-nutritional-quality diet.
“…Serum total cholesterol (TC) levels and triglyceride (TG) were measured using assays kits BC1985 and BC0625 acquired from Solarbio Company (Beijing, China) according to the methods of [28,29], following the manufacturer's protocols. The method of [30] was followed to assess the serum HDL-C (high-density lipoprotein cholesterol) levels, while the other lipid parameters, such as LDL-C (high-density lipoprotein cholesterol) and very LDL-C, were mathematically considered following the technique depicted by [31].…”
High levels of lipids and cholesterol, particularly LDL, in blood are considered the most common risk factors for cardiovascular diseases that threaten human life. Recently, interest has increased in the use of medicinal plants to treat various diseases due to their lack of side effects. The current study aims to investigate the effect of Ajwa date (AD) and germinated barley (GB) on the lipid profile in rats fed a high-fat diet (HFD). Thirty rats were distributed into five groups (six per group) as follows: the negative control group, the positive control group fed a HFD, and the other three groups that were fed a HFD supplemented with a mixture of AD and GB in equal ratios with different proportions of 20, 30, and 40% in the diet. There was an increase in moisture, protein, phenols, and vitamin C content and a decrease in the content of ash, carbohydrates, fats, and beta-glucans in GB. Blood total cholesterol levels decreased significantly (83.53, 70.12, and 73.55 mg/dL) in the groups fed the AD and GB mixtures in different percentages (20, 30, and 40%). Likewise, the AD and GB mixtures recorded a significant decrease in the level of triglycerides in the treated groups, with no significant effect on the high-density lipoprotein and low-density lipoprotein. There was a significant improvement in the level of alanine aminotransferase, while the level of aspartate aminotransferase was not affected by the treatment. In summary, AD and GB mixtures can modulate the lipid profile alterations caused by HFD through their phytochemical constituents, particularly beta-glucans.
“…In vitro studies have shown that ADe polysaccharide effectively scavenges ROS, highlighting its antioxidative effects [27]. Our previous investigation revealed that ADe can exert anti-obesity effects in mice fed with high-fat diets by modulating oxidative stress [28]. However, the inhibitory effect of ADe on CAC, along with a lack of comprehensive understanding of underlying mechanisms.…”
Background: The edible fungus Auricularia delicate (ADe) is commonly employed in traditional medicine for intestinal disorders; however, its inhibitory effect on colitis-associated colorectal cancer (CAC) and the underlying mechanisms remain unexplored. (2) Methods: The inhibitory effect of ADe on CAC was investigated using a mouse model induced by azoxymethane/dextran sulfate sodium. Results: ADe effectively suppressed the growth and number of intestinal tumors in mice. Intestinal microbiota analyses revealed that ADe treatment increased Akkermansia and Parabacteroides while it decreased Clostridium, Turicibacter, Oscillospira, and Desulfovibrio. ADe regulated the levels of 2′-deoxyridine, creatinine, 1-palmitoyl lysophosphatidylcholine, and choline in serum. Furthermore, the levels of these metabolites were associated with the abundance of Oscillospira and Paraacteroides. ADe up-regulated the free fatty acid receptor 2 and β-Arrestin 2, inhibited the nuclear factor kappa B (NF-κB) pathway, and significantly attenuated the levels of inflammatory cytokines, thereby mitigating the inflammatory in CAC mice. Conclusions: The protective effect of ADe in CAC mice is associated with the regulation of intestinal microbiota, which leads to the inhibition of NF-kB pathway and regulation of inflammation.
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